Gene Information

Name : lysS (A9601_18281)
Accession : YP_001010218.1
Strain : Prochlorococcus marinus AS9601
Genome accession: NC_008816
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 1559871 - 1561337 bp
Length : 1467 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
GTGAATAAAGGATTTGCTTCTTACGCACAAAGCTTTAAGGTATCACATACTACCAGTTTTCTTATTCAAAAATTTGATCA
TCTAGAAAATGGTCAAGAGGAAGACTTCAGTGTTTCTATTGCTGGTAGAGTTCTGGCAAAAAGGGTAATGGGCAAAATTG
CCTTTTTCACAATAAGCGATCAAGAAGGTCAGATTCAGCTTTATCTAGATAAAAGGATTATTAATTTCAATTTAGAAAAA
CAAAAATTACTTTCTTTTGAAGATCTCAAAGAAATAGTAGATATTGGTGATTGGATAGGAGTCTATGGAACTATTAAAAA
AACTAATAAAGGTGAGCTTTCAATTAAAGTAGAAAAATGGGAAATGTTATCCAAATCATTACAACCTCTCCCAGATAAAT
GGCATGGATTGACTGATATTGAAAAAAGATATAGACAACGTTATTTAGATTTAATAGTTAATCCTCACTCTAAAAATGTA
TTTAAAACCAGAGCGAAATGTATAAGTTTTATAAGAAAATGGCTAGATAATAGAAATTTTTTAGAGATAGAGACTCCAAT
TCTGCAATCTGAAGCTGGTGGTGCTGAAGCAAGACCATTTATAACTCATCACAATACATTAGATATTCCGTTGTATTTAA
GAATAGCTACAGAATTACATTTAAAGCGAATGGTTGTTGGAGGTTTTGAGAAAGTCTATGAATTGGGAAGAATCTTCCGT
AATGAGGGGATAAGTACAAGGCATAATCCAGAATTCACCTCAGTGGAAATTTATGAAGCTTATTCTGATTATGTAGATAT
GATGAATTTAACTGAAGAATTGATTAAAGATATCGTAGCTGATGCATGTGGGTCCTTAATTATAAATTATCAAAATAAAG
AAATTGATTTTTCTAAGCCTTGGTCAAGAATATCCATGAAAGCTATTGTCAAAAAATATACAGGGATTGATTTTGATTCT
TTCAGTGGAGACTTTCTAGCAGCAAAACAAGCCGTTAAAAATATCAATGTTGATTGTTCTAATAAAGTAAATACTATGGG
AAGACTTTTAAATGAGGTCTTCGAGCAAAAAGTAGAATCAAAACTTGTAGAACCCACTTTTGTTATTGATTATCCTGTTG
AAATTTCTCCTTTAGCTAGGCCTCATCATGATAATAAACAAATAGTTCAGAGATTTGAATTATTCATTGTTGGTAGAGAA
CTGGCAAATGCGTTTAGTGAGTTGATAGATCCAGTAGATCAAAGAGAAAGAATGCAATTACAGCAATCTCTTAGAGACGA
AGGAGATCTTGAGGCTCACTGTATAGATGAAGATTTTTTAAATGCTTTAGAGATTGGCATGCCGCCTACGGGAGGATTAG
GTATAGGCATTGATAGGCTAATTATGTTAATTACTAATAGCGCATCGATTAGAGATGTAATCCCTTTCCCATTGTTAAAA
CCAGAAATAACTTCCAAAAAAAGTTAA

Protein sequence :
MNKGFASYAQSFKVSHTTSFLIQKFDHLENGQEEDFSVSIAGRVLAKRVMGKIAFFTISDQEGQIQLYLDKRIINFNLEK
QKLLSFEDLKEIVDIGDWIGVYGTIKKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPHSKNV
FKTRAKCISFIRKWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMVVGGFEKVYELGRIFR
NEGISTRHNPEFTSVEIYEAYSDYVDMMNLTEELIKDIVADACGSLIINYQNKEIDFSKPWSRISMKAIVKKYTGIDFDS
FSGDFLAAKQAVKNINVDCSNKVNTMGRLLNEVFEQKVESKLVEPTFVIDYPVEISPLARPHHDNKQIVQRFELFIVGRE
LANAFSELIDPVDQRERMQLQQSLRDEGDLEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLITNSASIRDVIPFPLLK
PEITSKKS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-99 46
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 1e-79 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001010218.1 lysyl-tRNA synthetase VFG1668 Protein 8e-100 46