Gene Information

Name : ysaN (YE3544)
Accession : YP_001007702.1
Strain : Yersinia enterocolitica 8081
Genome accession: NC_008800
Putative virulence/resistance : Virulence
Product : ATP synthase SpaL
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 3860424 - 3861716 bp
Length : 1293 bp
Strand : -
Note : Invasion protein InvC; necessary for efficient entry of S.typhimurium into cultured epithelial cells. Probable catalytic subunit of a protein translocase

DNA sequence :
ATGAATCTCTTTGATAGCTGTGCACACCCGTCGCGTATACATGGTTGCCTGCTAGAGGCCCCGCTGCATGGGGTCTTTAT
TGGCGAAATCTGTCTGATTGAGCGCGATTTATGTCAGCCGGAAGTGATTGCAAAAGCTCAGGTCGTGGGGTTTAAGGAGG
GACAAACCATTCTGAGTTTGATTGGTCGAGCACAGGGGCTGACACGTGAAGTGGTTATCCGCCCCACTGGTCAGCCGTTT
GTCTTTGAGATGGGAGAACATCTGGCGGGTAAAATTTACAACGCCGCAGGAGAGGAAGTGGGCGTGCTGAGCAATGCAAC
TGCATCATCAGAACCTCTATTCACCACTCTGTGTCGGGTTGATAATCCGCCGGTTAGCGTCAATTTGCGCCGACCGGTTA
CCACACCATTGGTGACGGGGGTCAGGGCAATTGATGGCTTGCTCACTTGTGGGCAGGGGCAGCGTATGGGGATTTTTGCG
GCGGCCGGTAGCGGCAAAACCTCCCTGATGAGCATGATTATGAATCATGCGGTGGCGGATATTTGTGTTATTGCGCTAAT
TGGTGAGCGCGGTCGTGAGGTGACAGAATTCATTCATGAATTGCAGACCTCACCGCGTGCTGCGCAAACCATTCTGGTGT
ACGCCACGTCGGACAGTCCAGCGGTTGAGCGCTGTAATGCAGCTTTACTGGCAACGGCGATGGCGGAGTATTTTCGTGAT
CAGGGCAAAGATGTGTTGCTGTTTGTCGATTCGATGACGCGTTATGCCCGTGCTTTGCGCGATGTAGCATTGGCGGCGGG
GGAACTTCCCGCACGGCGCGGTTACCCAGCGTCGGTATTCGAGCAACTGCCACTGTTATTGGAACGGCCCGGTGCATTGC
AGCATGGCTCTATTACGGCTTTTTATACCGTACTGCTGGAGAGCGAAGAGGAATCCGACCCGATTGGTGATGAAATTCGC
TCGATAATCGATGGTCACATCTACCTTAGTGCACAACTCGCTGGGCGCGGTCACTACCCAGCAATTGATATCTTACACAG
TATTAGTCGTGTGTTTTCGAAAGTGACTACACCGCAGCATCGTCAGGATGCGGCAAAAACGCGCGATATGCTCGGACGGC
TGGCACAAATTCAGCTCTATCTTGATCTCGGGGAGTACCAGCGTGGTGAAAATACCGACAACGATCACGCGCTGGACAAC
AGAGATGTCATAGAGGGCTTTTTGCAGCAAGCAATGGAAGAACCCGGCGAGTTCTCCACCACGCTCAATCAGCTCCGTGA
GCTGGCAAACTGA

Protein sequence :
MNLFDSCAHPSRIHGCLLEAPLHGVFIGEICLIERDLCQPEVIAKAQVVGFKEGQTILSLIGRAQGLTREVVIRPTGQPF
VFEMGEHLAGKIYNAAGEEVGVLSNATASSEPLFTTLCRVDNPPVSVNLRRPVTTPLVTGVRAIDGLLTCGQGQRMGIFA
AAGSGKTSLMSMIMNHAVADICVIALIGERGREVTEFIHELQTSPRAAQTILVYATSDSPAVERCNAALLATAMAEYFRD
QGKDVLLFVDSMTRYARALRDVALAAGELPARRGYPASVFEQLPLLLERPGALQHGSITAFYTVLLESEEESDPIGDEIR
SIIDGHIYLSAQLAGRGHYPAIDILHSISRVFSKVTTPQHRQDAAKTRDMLGRLAQIQLYLDLGEYQRGENTDNDHALDN
RDVIEGFLQQAMEEPGEFSTTLNQLRELAN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-110 57
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 6e-110 57
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-110 57
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-110 57
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 3e-112 56
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 3e-106 55
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-103 53
escN CAC81862.1 EscN protein Virulence LEE II Protein 5e-71 50
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 6e-71 50
escN YP_003223475.1 translocator EscN Virulence LEE Protein 5e-71 50
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-71 50
escN AAK26715.1 EscN Virulence LEE Protein 1e-70 50
escN AAL57542.1 EscN Virulence LEE Protein 1e-70 50
escN NP_290268.1 hypothetical protein Virulence LEE Protein 8e-71 49
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 6e-71 49
ECs4568 NP_312595.1 EscN Virulence LEE Protein 8e-71 49
escN AAC38383.1 EscN Virulence LEE Protein 5e-71 49
escN YP_003236088.1 translocator EscN Virulence LEE Protein 4e-71 49
escN AAC31513.1 L0034 Virulence LEE Protein 6e-71 49
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-69 48
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-71 46
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-71 46
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-71 46
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-71 46
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 5e-73 46
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 5e-73 46
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-85 45
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 3e-73 45
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-72 45
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 4e-69 44
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 4e-76 43
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-76 43
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-78 43
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-78 43
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-78 43
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 7e-78 43
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-77 43
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-78 43
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-78 43
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 8e-69 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 4e-69 42
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 1e-62 41
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-58 41
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 8e-63 41
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 3e-69 41
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 8e-73 41
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-63 41
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-63 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ysaN YP_001007702.1 ATP synthase SpaL VFG1778 Protein 0.0 99
ysaN YP_001007702.1 ATP synthase SpaL VFG0555 Protein 3e-110 57
ysaN YP_001007702.1 ATP synthase SpaL VFG2459 Protein 6e-114 56
ysaN YP_001007702.1 ATP synthase SpaL VFG1008 Protein 1e-99 54
ysaN YP_001007702.1 ATP synthase SpaL VFG0730 Protein 3e-71 49
ysaN YP_001007702.1 ATP synthase SpaL VFG0812 Protein 3e-71 49
ysaN YP_001007702.1 ATP synthase SpaL VFG0515 Protein 2e-71 46
ysaN YP_001007702.1 ATP synthase SpaL VFG0390 Protein 3e-85 45
ysaN YP_001007702.1 ATP synthase SpaL VFG0192 Protein 2e-87 45
ysaN YP_001007702.1 ATP synthase SpaL VFG2144 Protein 3e-75 44
ysaN YP_001007702.1 ATP synthase SpaL VFG0048 Protein 1e-79 43