Gene Information

Name : Pnap_4001 (Pnap_4001)
Accession : YP_984214.1
Strain : Polaromonas naphthalenivorans CJ2
Genome accession: NC_008781
Putative virulence/resistance : Resistance
Product : heavy metal translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 4264289 - 4266526 bp
Length : 2238 bp
Strand : -
Note : TIGRFAM: ATPase, P-type (transporting), HAD superfamily, subfamily IC; cadmium-translocating P-type ATPase; heavy metal translocating P-type ATPase; PFAM: Haloacid dehalogenase domain protein hydrolase; Heavy metal transport/detoxification protein; E1-E2

DNA sequence :
ATGACCGACTGCTGCAACCAACCCTCTCACACGTCCGCCATTGCCGACCTTCCGGCCGCGCCTGTTCCCGCCGGCGCCAC
GCGCTTTCGCATCCCGGCGATGGACTGCGCGGTTGAAGAGGCCGACATCCGGCGCGTGCTGGCCGGCGTGCCGGGCATCC
GCTCGCTCAACTTTCAGCTGGTGGCGCGCACGCTGACGATTGATGCGCAGGCCGAAGCCCTTCAGGCGGCGGTCAGCGCG
ATAGGCCAGGCGGGCTACAAGATGCAGCCGTTTACCGACGCCGGGCCAGCCGGCCAAACCCATGGTGCGCACGACCATGA
GCACGATCACGCGCACGGCCACGGCGGCCTGCCCTCGGGCCTGCCGCGCCTGGCGGGCGCGCTGGGGCTGGCCGGGGTGG
CCGAGGTGATCAGCTTTTTCGCCCCCGCCGGCCTGCCCTGGACGCTGGCCGGCATGGCGGTGGCGCTGGCCGCCATCTTC
ATGGCCGGGCTGGAGGTGTACCAGAAAGGCCTCACGGCCTTGTTCAGGCGCCGGCTCAACATCAATGCGCTGATGACGGT
GGCCGTCACCGGCGCCTTCCTGATCGGCCAGTGGCCCGAGGCGGCCATGGTGATGGCGCTGTATTCCATCGCCGAGCTGA
TCGAGGCCATGGCGGTGGACCGCGCGCGCAATGCCATCAAGGGCCTGATGGCACTGGCGCCCGAAGCCGCCGAGCTTCGC
CAGCCGGACGGCCAGTGGACCAGCGTGCCGCTCAATGACGTGCCGCTGGGCGCCACGGTGCGCGTGCGGCCCGGCGGGCG
CATTCCGCTGGACGGCCTGGTCATGGCCGGCGCCAGCGCGGTTGATCAGGCGTCGGTCACAGGGGAAAGCCTGCCGGTGG
ACAAGGCGGCGGGCGACAGCGTGTTCGGCGGCACCATCAGCCAGACCGGCGCGCTGGAGCTGCGGGTCACGGCGGCCGCC
AGCGACAGCACGCTGGCGCGCATCATCCACGCCGTCGAGCAGGCGCAGGGCGCGCGCGCGCCCACGCAGACCTTCATCGA
CCGCTTCGCCGCCATCTACACGCCGGCGGTGTTCATGCTGGCGCTGGCCGTGGCCGTCCTGACGCCGCTGCTGCTTGACT
GGACCTGGCTGGAGGCGCTGTACAAGGCGCTGGTGCTGCTGGTGATTGCCTGCCCGTGCGCGCTGGTGGTGTCCACGCCG
GTCACGGTCGTCAGCGGCCTGACCACGGCGGCGCGGCGCGGCATCTTGATCAAGGGCGGCACCTACCTCGAAGACGCCCG
GCGGCTGAAGGCCGTGGCGCTGGACAAGACCGGCACCATCACCCAAGGCAAGCCGGTGCTGGTGGCCTGGCAGGCATGGG
GCGGCGCGGATCAGGCCGAAGTCGGCCGCCTGGCGGCCACGCTGGCGGCGCGCTCCGACCATCCGGTGTCCAGCGCCATC
GCGCAGGGGCTGCGGGCCGGCAACCCGCAAGGCGCCCTGCCGGCAGGGGCAGTTGAGGCGGTCGAGATGCTTGAGGCGCT
GTCGGGGCGGGGCGTCCAGGGCCGGATCGGTGGACAACCGTACGCGCTGGGCAACCACCGGCTGATGGAGGAGCGCCAGC
AATGCTCGCCCGCGCTGGAAGCGGCGCTGGCCCTGCACGAGCAGCAGGGCCGCACGGTCACGCTGCTGGCCAGCGCGCAG
GGCGTGCTGGGAATCTTCGCGGTGGCCGACACCATCAAGGAAAGCTCGCGCCAGGCGATTGCCGAGCTGAAGGCGCTGGG
CGTGACGCCCGTCATGCTCAGCGGCGACAACGCCGCCACGGCCAGCGCGGTGGCGGCGCAGGCCGGCATCATTGACGTGC
GCGGCAACCTGCTGCCCGAGGAAAAACTGGCCGCCATCCGGCAGCTGCAAGGCCAGTACGGCGCGACCGGCATGATCGGC
GACGGCATCAACGACGCGCCCGCGCTGGCGCAGGCCGACATCGGTTTTGCCATGGGCGGCGCGGGCACCGACATTGCCAT
CGAGGCGGCCGACGTGGTCATCATGAACGACAACCTGCGGCGCATCGCCGAGACGGTGCGCCTGTCGCGGCGCACGCATG
CCGTGCTGTGGCAAAACATCGCGCTGGCCCTGGGCATCAAGGCGGTGTTTTTCGTGCTGGCCGTGTTCGGCAGCGCGACC
ATGTGGATGGCTGTGTTCGCCGACATGGGCGCCAGCCTGCTGGTGGTGGCCAACGGCCTGCGGCTGCTGCGCCGCTAG

Protein sequence :
MTDCCNQPSHTSAIADLPAAPVPAGATRFRIPAMDCAVEEADIRRVLAGVPGIRSLNFQLVARTLTIDAQAEALQAAVSA
IGQAGYKMQPFTDAGPAGQTHGAHDHEHDHAHGHGGLPSGLPRLAGALGLAGVAEVISFFAPAGLPWTLAGMAVALAAIF
MAGLEVYQKGLTALFRRRLNINALMTVAVTGAFLIGQWPEAAMVMALYSIAELIEAMAVDRARNAIKGLMALAPEAAELR
QPDGQWTSVPLNDVPLGATVRVRPGGRIPLDGLVMAGASAVDQASVTGESLPVDKAAGDSVFGGTISQTGALELRVTAAA
SDSTLARIIHAVEQAQGARAPTQTFIDRFAAIYTPAVFMLALAVAVLTPLLLDWTWLEALYKALVLLVIACPCALVVSTP
VTVVSGLTTAARRGILIKGGTYLEDARRLKAVALDKTGTITQGKPVLVAWQAWGGADQAEVGRLAATLAARSDHPVSSAI
AQGLRAGNPQGALPAGAVEAVEMLEALSGRGVQGRIGGQPYALGNHRLMEERQQCSPALEAALALHEQQGRTVTLLASAQ
GVLGIFAVADTIKESSRQAIAELKALGVTPVMLSGDNAATASAVAAQAGIIDVRGNLLPEEKLAAIRQLQGQYGATGMIG
DGINDAPALAQADIGFAMGGAGTDIAIEAADVVIMNDNLRRIAETVRLSRRTHAVLWQNIALALGIKAVFFVLAVFGSAT
MWMAVFADMGASLLVVANGLRLLRR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 1e-115 42
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 5e-114 42
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 4e-114 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Pnap_4001 YP_984214.1 heavy metal translocating P-type ATPase BAC0298 Protein 3e-161 62