Gene Information

Name : lysS (Sputw3181_0896)
Accession : YP_962300.1
Strain : Shewanella sp. W3-18-1
Genome accession: NC_008750
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 1021858 - 1023360 bp
Length : 1503 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACTGAACAAGTACAAGATGAAAACAAACTGATCGCCGAGCGTCGTGCCAAGCTAGACAGCATTCGCCCAAATTGTAG
CGCTAATGCTCACCCTAATACCTTCCGTCGCACCCATAAAGCGGCTGAGTTACAGGAGAAATACGGCCAAAACACTAAGG
AAGAACTCGAAGCCTTAGGTTTTAGAACCAGCATTGCTGGTCGTATCATGGCAAAACGTGGCCCATTCCTCGTAATTCAA
GACGTGTCTGGTCGTATCCAAGCTTATGCTGAAAAGGGCGTTCAAGCGGATCTGAAGGATCGTTACCAAGGTCTGGATAT
CGGTGACATCATCGGCGTAACTGGTCAGTTACACTTGTCTGGTAAAGGCGATCTGTACGTCAATATGGAAGAGTACCAAC
TGCTGACCAAAGCACTGCGTCCACTGCCAGAGAAATTCCACGGCTTAACCGACCAAGAAACCCGTTACCGCCAACGTTAT
GTTGACTTAATCGTTAACGAAGAATCTCGTCAAGCCTTCGTGATGCGCTCAAAAGTGGTTGCAGCTATCCGTAACTTCAT
GATCAAAAAAGAGTTCATGGAAGTTGAAACCCCAATGATGCACGTGATCCCTGGCGGCGCTTCAGCCCGTCCATTTATCA
CGCACCACAATGCGCTGGACATGCCAATGTACCTGCGTATCGCGCCAGAGCTATACCTCAAGCGTTTAGTGGTCGGTGGT
TTTGAGCGTGTGTTCGAAATCAACCGTAACTTCCGTAACGAAGGTCTGTCGCCACGCCATAACCCAGAATTCACTATGAT
GGAATTCTATATGGCTTACGCTGATTACAAAGATTTGATGGACTTAACCGAAGAACTGTTAAGCTCAATCGCGATCGAAT
TGTTAGGCAGTGCGCAAATGCCATACGGCGAGCACACCGTTGATTTCGGCGGCCCATATGCCCGTCTGAGCATGTTAGAA
GCGATTCAGAAGTACAATCCTGATAACGCGACTATCCAAGCCATGACCTACGAGCAAGTGAAAGATCTCGAATTTATGCG
TGAATTGGCTATCAGCCTAGGTATCAAGATCGAGAAGTTCTGGACCTGTGGTCAGCTATTAGAAGAAATCTTCGGTGAAA
CTGCTGAATGGCAATTAATGCAACCGACCTTCATCACTGGCTACCCAGCGGACATTTCGCCACTGGCACGTCGTAACGAT
GACAATCACTTCATCACCGACCGTTTTGAATTCTTCATCGGTGGTCGTGAAGTAGCTAACGGTTTCAGCGAGTTAAATGA
TGCTGAAGATCAAGACAGCCGCTTTAAAGCGCAGGTTGATGCCAAAGACGCGGGTGACGATGAAGCCATGTTCTACGATG
CGGACTATATCACTGCACTGGAACACGGTTTACCACCAACAGCGGGTCAAGGTATTGGTATCGACCGTTTAGTGATGCTA
TTCACTAACACTCATACAATCCGTGATGTGATCCTATTCCCTGCAATGCGCCCACAAGCCTAA

Protein sequence :
MTEQVQDENKLIAERRAKLDSIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALGFRTSIAGRIMAKRGPFLVIQ
DVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEESRQAFVMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQMPYGEHTVDFGGPYARLSMLE
AIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKFWTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRND
DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 5e-130 57

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_962300.1 lysyl-tRNA synthetase VFG1668 Protein 3e-130 57