Gene Information

Name : fliI (Sputw3181_1430)
Accession : YP_962827.1
Strain : Shewanella sp. W3-18-1
Genome accession: NC_008750
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 1617712 - 1619049 bp
Length : 1338 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGCAAAACCGTCAACACCAGCTGCTGAGTAAGTTCCACCAATATATTGATAAAGTACCTCCTTTTCGCGCCGTTGCCAG
TGGGCAATTGGTGCGGGTTGTTGGTCTCACTTTAGAGGCGAGTGGTTGCCGTGCGCCTGTTGGTAGTTTGTGTTCTATCG
AAACCATGGCGGGAGATTTAGTGGCTGAGGTGGTCGGGTTTGATGATGAGTTACTCTATCTTATGCCCATTGAAGAGTTA
CGAGGTGTACTCCCTGGAGCGAGAGTCATTCCACTTGGAGAGCAAACAGGGCTTAAAGTAGGCTTATCTTTATTAGGCCG
TGTACTCGATGGCAATGGTGTTCCTCTTGATGGATTAGGTGCGCTGCAAACCGATCAATATGCACCAAGACATAATTCTG
CCATTAATCCCTTATCTCGCCGTGCAATAACGGAACCCCTTGATGTTGGCGTACGTGCGATTAATGCCATGTTGACCGTT
GGTAAAGGTCAGCGTATGGGACTCTTTGCGGGCTCTGGTGTCGGTAAAAGTGTGCTTCTGGGTATGATGACCCGTGGTAC
TACTGCAGATATTATTGTGGTGGGGTTGGTTGGTGAACGGGGACGAGAAGTTAAAGAATTTATTGAAGAAATTTTGGGTG
AAAAGGGGCGAGCTCGCTCCGTTGTTATTGCGGCTCCGGCGGATACCTCACCCTTAATGCGCCTTCGCGCCTGTGAAACG
TCAACGCGTATCGCTGAGTATTTCCGTGATTTAGGCTATAACGTGTTGTTATTGATGGATAGCTTGACTCGATATGCCCA
AGCGCAGCGGGAAATCGCTTTAGCGGTGGGGGAGCCGCCTGCAACAAAAGGTTATCCACCATCAGTATTTGCTAAATTGC
CCCGTCTTGTCGAACGTGCGGGTAATGGTGGTCCTGGACAGGGATCTATTACGGCGTTTTATACCGTATTGACCGAAGGC
GATGATCAACAAGATCCGATTGCCGATGCATCAAGAGCGATTCTGGATGGCCATATCGTATTATCGCGTGCTCTGGCTGA
TTCGGGGCACTATCCCGCGATTGATATTGAAGCATCAATTAGCCGTGTTGCTCCGATGGTTATTTCTAATGAGCATTTAG
AAGCGATGCGCCGTGTAAAACAAACCTATTCTTTATATCAACAGAATCGAGATTTAATCTCTATTGGCGCCTACGCCCAA
GGTAGTGATCCTCGGATCGATAATGCGATACGTCTGCAACCGGCGATGAATGCCTTCTTAAGGCAAACCATGCGTGATGC
CTTTAGCTTTGCAGATAGTCAATCGATGCTGGCTCAACTCGCTGCACAGTGTAAATAA

Protein sequence :
MQNRQHQLLSKFHQYIDKVPPFRAVASGQLVRVVGLTLEASGCRAPVGSLCSIETMAGDLVAEVVGFDDELLYLMPIEEL
RGVLPGARVIPLGEQTGLKVGLSLLGRVLDGNGVPLDGLGALQTDQYAPRHNSAINPLSRRAITEPLDVGVRAINAMLTV
GKGQRMGLFAGSGVGKSVLLGMMTRGTTADIIVVGLVGERGREVKEFIEEILGEKGRARSVVIAAPADTSPLMRLRACET
STRIAEYFRDLGYNVLLLMDSLTRYAQAQREIALAVGEPPATKGYPPSVFAKLPRLVERAGNGGPGQGSITAFYTVLTEG
DDQQDPIADASRAILDGHIVLSRALADSGHYPAIDIEASISRVAPMVISNEHLEAMRRVKQTYSLYQQNRDLISIGAYAQ
GSDPRIDNAIRLQPAMNAFLRQTMRDAFSFADSQSMLAQLAAQCK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-84 50
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 3e-84 50
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 3e-84 50
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 6e-79 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-83 49
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-84 49
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-83 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 6e-84 49
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-80 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-80 49
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 7e-70 49
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-69 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-70 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 8e-70 49
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-70 49
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-61 48
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-65 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-73 47
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-64 47
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-64 47
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-66 46
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-66 46
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 8e-67 46
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 4e-71 46
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 8e-65 45
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-64 45
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 7e-69 45
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-64 45
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-71 45
escN AAK26715.1 EscN Virulence LEE Protein 5e-71 45
escN AAL57542.1 EscN Virulence LEE Protein 5e-71 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 4e-71 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 3e-71 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-70 45
escN NP_290268.1 hypothetical protein Virulence LEE Protein 6e-71 45
ECs4568 NP_312595.1 EscN Virulence LEE Protein 6e-71 45
escN AAC31513.1 L0034 Virulence LEE Protein 4e-71 45
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-71 45
escN AAC38383.1 EscN Virulence LEE Protein 5e-71 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-69 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-69 44
unnamed AAL06369.1 EscN Virulence LEE Protein 6e-69 43
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 3e-65 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_962827.1 flagellum-specific ATP synthase VFG1254 Protein 3e-123 65
fliI YP_962827.1 flagellum-specific ATP synthase VFG2331 Protein 7e-107 63
fliI YP_962827.1 flagellum-specific ATP synthase VFG2499 Protein 4e-111 61
fliI YP_962827.1 flagellum-specific ATP synthase VFG0515 Protein 4e-70 49
fliI YP_962827.1 flagellum-specific ATP synthase VFG0048 Protein 2e-79 48
fliI YP_962827.1 flagellum-specific ATP synthase VFG0192 Protein 3e-77 46
fliI YP_962827.1 flagellum-specific ATP synthase VFG0390 Protein 7e-73 46
fliI YP_962827.1 flagellum-specific ATP synthase VFG2144 Protein 6e-78 45
fliI YP_962827.1 flagellum-specific ATP synthase VFG0812 Protein 2e-71 45
fliI YP_962827.1 flagellum-specific ATP synthase VFG0730 Protein 3e-71 44
fliI YP_962827.1 flagellum-specific ATP synthase VFG2459 Protein 7e-61 41