Gene Information

Name : entE (Pden_2386)
Accession : YP_916171.1
Strain :
Genome accession: NC_008686
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 2382798 - 2384420 bp
Length : 1623 bp
Strand : -
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGATTGAATTCAGCCCTTGGCCGCAAGACCTCGCGCAGCGCTATCGCGAGGCCGGTTATTGGATCGACCAGCCGCTGAC
CCATATCCTGGACGCGCAGCTGGCCGCCAACCCCGAGGCCGAGGCGCTGGTCTGCGGTTCGCGCCGCTTCAGCTATGCCG
ATCTGGACCGGCTGTCGTGCAACCTGGCCGCCAGGCTGGCGCAGCAGGGGCTGGGCCATGGCGATACGGCGCTGGTGCAA
TTGCCGAACGTGGCCGAGTTCTTCGTCGCCTTCTTCGCGCTTCTGCGGATCGGCGTGGCGCCGGTGAACGCGTTGTTCAG
CCATCGCCGGCTGGAGCTTGCGGAATATGTCCGCCAGATCGCGCCGCGCGTGGTCATTGCCTCGCGCCAGCACGAGCTGT
TCCGCGACGACGCCTTTGCCGAGGAATTGCGGGCGGGTGGCGTCGCGCATCTGCTGCTGCTGGGCGAGGACGACCCCCAG
CGCAGCCTGGATCACTGGCTGACCCATGGCGGTGACCTGCCGGCGGGGGCGGGGCCGACCCCGGGCGGCGAGGTCGCCTT
CTTCCAGTTGTCGGGCGGCAGCACCGGGACGCCCAAGCTGATCCCGCGCACCCATAACGATTACGACTACAGCATCCGCG
CCAGCGTCCAGATCTGCGGCGTGACCTCGGCCACCCGCTTTCTTTGCGCCTTGCCGGCGGGGCACAACTTCACCATGAGC
TCGCCCGGCGCGCTGGGGGTGTTCCATGCCGGCGGCACGGTGGTCATGGCCTCCAGCCCCGAACCGCTGACCTGCTTCCA
GATCGTGGCGCGCGAACGGATCACCATGGCGCCGCTGGTGCCGCCCGCCGTCGCGCTGTGGCTGCGCGCGGTCGAGGAAG
AGCCCGCCCGCCGCGCCATGCTGGACAGCCTGGAGCTGATGCTGGTCGGCGGCGCCAGTTTCGCCGAGGCGACGGCGCGG
CGCGTTCCCGAACTGCTGGGCTGCCGCCTGCAGCAGGTCTTCGGCATGGCCGAGGGGCTGGTGAACTATACCCGGCTGGA
CGATCCGCCCGAGGTGATTTTCACCACCCAGGGCCGGCCGATCAGCCCCGACGACGAGGTGCGCATCGTCGACGAGGACG
GCTGCGACGTGCCGGACGGCACGCCCGGCGCGCTGGCAACCCGCGGCCCCTATACCTTCCGCGGCTACTACCGCAGCCCC
GAGCACAATGCGAAGTCCTTTGACGCCGAGGGGTTCTACTATTCGGGGGACGTGGTGGTGCGGCAGGGCGGCAACCTGCG
CGTCGTCGGCCGCATCAAGGACCAAATCAACCGGGGCGGCGAAAAGATCGCCGCCGAGGAAATCGAGAACCTGCTGGTGC
GCCACCCCGACATCACCCATGCCGCGCTGGTCGCCGCCCCCGACGCGCATCTGGGCGAGAAAAGCTGCGCCTTCCTGGTG
CTGCGCGAGGGGTCGGGCAAGCTTTCCTCGCCGGCGCTGCGCCGGCACCTGCTGGAACTGGGCGTGGCCGAATACAAGCT
GCCCGACCGTTTCCGCTTCGTGCCCGAACTGCCGCTGACGGCGGTGGGCAAGATCGACAAGCGCCGCCTGCGCGACAGCC
TCGTGGCCGAAACCGCCGCCTGA

Protein sequence : 
MIEFSPWPQDLAQRYREAGYWIDQPLTHILDAQLAANPEAEALVCGSRRFSYADLDRLSCNLAARLAQQGLGHGDTALVQ
LPNVAEFFVAFFALLRIGVAPVNALFSHRRLELAEYVRQIAPRVVIASRQHELFRDDAFAEELRAGGVAHLLLLGEDDPQ
RSLDHWLTHGGDLPAGAGPTPGGEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRASVQICGVTSATRFLCALPAGHNFTMS
SPGALGVFHAGGTVVMASSPEPLTCFQIVARERITMAPLVPPAVALWLRAVEEEPARRAMLDSLELMLVGGASFAEATAR
RVPELLGCRLQQVFGMAEGLVNYTRLDDPPEVIFTTQGRPISPDDEVRIVDEDGCDVPDGTPGALATRGPYTFRGYYRSP
EHNAKSFDAEGFYYSGDVVVRQGGNLRVVGRIKDQINRGGEKIAAEEIENLLVRHPDITHAALVAAPDAHLGEKSCAFLV
LREGSGKLSSPALRRHLLELGVAEYKLPDRFRFVPELPLTAVGKIDKRRLRDSLVAETAA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-81 44
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-81 44
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-83 44
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 6e-83 44
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-83 44
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 5e-83 44
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-83 44
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-83 44
ybtE CAA21394.1 - Virulence HPI Protein 5e-83 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_916171.1 enterobactin synthase subunit E VFG0932 Protein 6e-135 55
entE YP_916171.1 enterobactin synthase subunit E VFG0168 Protein 7e-109 48
entE YP_916171.1 enterobactin synthase subunit E VFG1817 Protein 3e-77 42