Gene Information

Name : lysS (Cpha266_1739)
Accession : YP_912180.1
Strain : Chlorobium phaeobacteroides DSM 266
Genome accession: NC_008639
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 1964587 - 1966116 bp
Length : 1530 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACGCCTAATGAAAACCCGCAAAGTCCTGGGAACCCCCAGCAGCTCTCTCTTAACGACCAGATGAAAAGGCGTTTGGA
TGAACGACTCCACCTTGCAGAATCCGGAGTGAATCCATACCCTTGCCATTTTGACGTAACGCACTTTTCCGATTCGATCC
TCACTGACTTTCAGGATGATGCAAACGAAACCGTTTCTGTTGCCGGAAGAATCATGACGATCAGAAAAATGGGAAAAGCC
TCGTTTTTCCATCTGCAGGATGCCAACGGCAGAATCCAGATTTACCTGAAAAAAGATGATGTTGGCGAATCCTCCTACCA
GACATTCAAACTGCTCGATATCGGTGATATTGTCGGCGTAAAAGGCTACACCTTCAAAACAAAAACCGGTGAAATATCAG
TACACGCACAGGAATTCTCTTTGCTCAGCAAGTCGCTTCGCCCTATCCCGATAGCCAAGGAAAAAGAGATTGACGGACAG
AAAGTAGTCTATGACGCTTTCAGCGACAAGGAGCTCCGTTACCGCCAGCGCTATGTTGACCTTATCGTCAACCCCGATGT
AAGGCAAACCTTTATCAAGCGCTCCGCAATTGTCTCATTTATGCGCGGGTTTTTCACAGGGCAAGGGTGGCTTGAAGTTG
AAACCCCTATCCTTCAGCCGATTTACGGCGGTGCGGCAGCACGTCCATTCACCACGCATCACAATGCACTCGACATGCAG
CTCTATCTGCGCATTGCCAATGAGCTCTACCTGAAGCGACTCATTGTTGGCGGTTTTGACGGAGTCTTTGAGTTTGCAAA
AGATTTCCGTAACGAAGGCATTGACCGTTTTCACAATCCCGAATTCACCCAGGTTGAGCTCTATGTTGCATACAAGGATT
ACGAGTGGATGATGAAGCTGGTTGAAGAGCTTTTTTATCAGACAGCATGCGCAGTTAACAACTCGGATTCCGTTGTTTTT
CTCGGCAACGACATAAGTCTTAAACCTCCGTTCCGCAGACTCACCATAACGGACTCCATTAAAGAGTATTGCGGTATCGA
TATCACCGATAAAGATGAACATGAGCTTCGGTATATGGCCAGGGATCTTGGACTTGAGCTTGACCCGAAAATCAGCAGCG
GTAAAATCATCGACGAAATATTTGGTGAATTTGTTGAGCCAAAACTTATTCAACCAACATTTATTACCGATTATCCGGTG
GAAATGTCGCCTCTTGCCAAGGAGCATCGATCCAAACCAGGCTTTGTCGAGCGCTTTGAACTCATTGCCGGCGGCAAGGA
GCTCTGCAACTCATTTTCCGAACTCAACGACCCGGTCATTCAGCGAGAACGACTGGAAGCACAGGCAAAACTGCGTCAGC
GAGGCGATGAGGAAGCGATGATTGTCGATGAGGATTTTCTGCGAGCCCTCGAATACGGCATGCCGCCATGTGCGGGACTT
GGTGTAGGTGTCGACAGAATGGTCATGCTGCTCACCGGTCAGGACTCGATAAGAGATGTTATCTTCTTTCCGCACCTGAA
ACCGGAATAG

Protein sequence :
MTPNENPQSPGNPQQLSLNDQMKRRLDERLHLAESGVNPYPCHFDVTHFSDSILTDFQDDANETVSVAGRIMTIRKMGKA
SFFHLQDANGRIQIYLKKDDVGESSYQTFKLLDIGDIVGVKGYTFKTKTGEISVHAQEFSLLSKSLRPIPIAKEKEIDGQ
KVVYDAFSDKELRYRQRYVDLIVNPDVRQTFIKRSAIVSFMRGFFTGQGWLEVETPILQPIYGGAAARPFTTHHNALDMQ
LYLRIANELYLKRLIVGGFDGVFEFAKDFRNEGIDRFHNPEFTQVELYVAYKDYEWMMKLVEELFYQTACAVNNSDSVVF
LGNDISLKPPFRRLTITDSIKEYCGIDITDKDEHELRYMARDLGLELDPKISSGKIIDEIFGEFVEPKLIQPTFITDYPV
EMSPLAKEHRSKPGFVERFELIAGGKELCNSFSELNDPVIQRERLEAQAKLRQRGDEEAMIVDEDFLRALEYGMPPCAGL
GVGVDRMVMLLTGQDSIRDVIFFPHLKPE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-99 51
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 3e-82 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_912180.1 lysyl-tRNA synthetase VFG1668 Protein 1e-99 51