Gene Information

Name : MSMEG_4516 (MSMEG_4516)
Accession : YP_888788.1
Strain : Mycobacterium smegmatis MC2 155
Genome accession: NC_008596
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 6.3.2.-
Position : 4603836 - 4605512 bp
Length : 1677 bp
Strand : +
Note : identified by match to protein family HMM PF00501

DNA sequence : 
GTGACTCTGACCACGCCCCACCCCCGCCCCGAGCAATCCGAATCTGCAGCTCAGTCAAGCCTGCTGGCCGGATTCACGCC
GTTTCCGGCCGAGCGCGCACAGGCCTATCGGGCGGCCGGGTACTGGCGGGACCAGCTACTGGATTCGGTCCTGCGGACGG
CGGCCCGCACATGGCCGGACCACATCGCGGTGATCGATGCGGACCACCGGCACACCTACGCCGAACTCGACCGCCTCGCC
GACCGCGCCGCGGCAGGTATCGCGGGCCTGGGCATCCGCCCCGGCGACCGTGTGCTGGTCCAGCTGCCCAACACCGCGGA
GTTCGCCGTCGCACTGTTCGGCCTGCTGCGCGCGGGCGCGGTACCCGTGATGTGTCTGCCCGGGCACCGGCTGGCCGAGC
TCACGCATTTCGCCGAGGTCAGCTCGGCGGTGGCGCTCGTCGTGGCCGACACCGCAGGCGGTTTCGACCACCGCGACCTT
GCACGCGAACTCGTGAGGTCCCATCCGGACGTGCGTCACGTCCTCGTCGACGGCGACGCCGCGGAGTTCCTGTCCTGGGC
GGAGGTGACGCGTGCGGCGCCCGGGCCCGTCCCCGAGATCGCGCCCGATCCGGCCGCCCCGGCGCTGCTGCTCGTGTCGG
GCGGCACCACCGGTGCACCGAAACTCATCCCGCGCACACATCAGGACTACGTCTACAACGCCACGGCCAGTGCCGAGCTG
TGCCGGCTGACCGCCGACGACGTCTATCTCGTGGCGCTGCCTGCCGCCCACAACTTCCCACTGGCGTGCCCCGGCCTGCT
CGGTGCGATGACCGTCGGTGCCACCACGGTGTTCACCACCGACCCGAGCCCCGAGGCCGCGTTCGCCGCGATCGACGAGC
ACGGCGTCACGGCCACCGCACTGGTGCCTGCGCTGGCCAAGCTGTGGGCGCAGGCGTGTGCGTGGGAACCCTTGGCTCCC
AAGACGCTGCGCCTGCTCCAGGTGGGCGGGGCCAAGCTGGCTGCGCCGGACGCGGCGCTGGTGCGCGGGGCCCTCACCCC
GGGACTGCAGCAGGTGTTCGGCATGGCCGAGGGCCTGCTGAACTACACGCGCATAGGCGATCCCCCCGAGGTGCTGGAGA
ACACCCAGGGCCGCCCGTTGTCACCGGACGACGAGATCCGCATCGTCGACGAGGTCGGCAACGAGGTGCCGCCCGGCGCC
GAGGGGGAACTGCTGGTGCGCGGACCGTACACGCTCAACGGCTATTTCAACGCCGAGGCGGCCAACGAGCGCTCGTTCAG
CCCGGACGGCTTCTACCGCAGCGGCGACCGGGTACGCCGCTTCGCCGACGGCCCGCTCGCCGGGTATCTGGAGGTGACCG
GCCGGATCAAGGACGTCATCGTGCGCGGCGGCGAGAACGTCTCCGCACTCGATCTCGAAGAGCACCTGCTGACCCACCCG
AGCGTGTGGGCCGCCGCAGCGGTTGCGCTGCCCGACGAGTTCCTCGGCGAGAAGATCTGCGCCGTGGTCGTTTTCAACGG
ACCCCCGGTCAGCCTGGCCGAGTTGCACGCGCATCTGGAGCAGCGCGGCGTCGCTGCCCACAGCAGGCCCGACGCGCTGG
TCCCGATGCCGAGCCTGCCCACCACGGCCGTCGGCAAGATCGACAAGAAGGCGATCGTGCGTCAGCTCGGCGGGTAG

Protein sequence : 
MTLTTPHPRPEQSESAAQSSLLAGFTPFPAERAQAYRAAGYWRDQLLDSVLRTAARTWPDHIAVIDADHRHTYAELDRLA
DRAAAGIAGLGIRPGDRVLVQLPNTAEFAVALFGLLRAGAVPVMCLPGHRLAELTHFAEVSSAVALVVADTAGGFDHRDL
ARELVRSHPDVRHVLVDGDAAEFLSWAEVTRAAPGPVPEIAPDPAAPALLLVSGGTTGAPKLIPRTHQDYVYNATASAEL
CRLTADDVYLVALPAAHNFPLACPGLLGAMTVGATTVFTTDPSPEAAFAAIDEHGVTATALVPALAKLWAQACAWEPLAP
KTLRLLQVGGAKLAAPDAALVRGALTPGLQQVFGMAEGLLNYTRIGDPPEVLENTQGRPLSPDDEIRIVDEVGNEVPPGA
EGELLVRGPYTLNGYFNAEAANERSFSPDGFYRSGDRVRRFADGPLAGYLEVTGRIKDVIVRGGENVSALDLEEHLLTHP
SVWAAAAVALPDEFLGEKICAVVVFNGPPVSLAELHAHLEQRGVAAHSRPDALVPMPSLPTTAVGKIDKKAIVRQLGG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 6e-88 45
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-88 45
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 5e-88 45
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-88 45
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 7e-88 45
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-88 45
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 6e-88 45
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-88 45
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-88 45

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
MSMEG_4516 YP_888788.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 5e-173 71
MSMEG_4516 YP_888788.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 3e-91 46
MSMEG_4516 YP_888788.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 8e-81 42