Gene Information

Name : lysS (Shewana3_3296)
Accession : YP_870926.1
Strain : Shewanella sp. ANA-3
Genome accession: NC_008577
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3944815 - 3946317 bp
Length : 1503 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACTGAACAAGTACAAGATGAAAACAAACTGATTGCCGAGCGTCGTGCCAAGCTAGAAAGCATTCGCCCGAACTGCAG
CGCTAATGCTCACCCTAATACTTTCCGTCGCACCCACAAAGCGGCTGAGTTGCAGGAAAAATACGGTCAAAACACTAAGG
AAGAACTCGAAGCCTTAGGTTTTAAAACCAGCATCGCTGGCCGTATCATGGCAAAACGTGGCCCATTCCTGGTGATCCAA
GACGTGTCTGGTCGTATCCAAGCTTACGCTGAAAAGGGCGTTCAAGCGGATCTGAAAGACCGTTACCAAGGTCTGGATAT
CGGTGACATCATCGGCGTAACTGGTCAGTTGCACTTGTCTGGTAAAGGCGATCTGTACGTGAACATGGAAGAGTACCAAC
TGCTGACTAAAGCACTGCGTCCTCTGCCAGAAAAATTCCACGGCTTAACCGACCAAGAAACCCGTTACCGCCAACGTTAT
GTGGACTTAATCGTTAACGAAGAATCTCGTCAAGCCTTCGTCATGCGTTCAAAAGTGGTTGCAGCTATCCGTAACTTCAT
GATCAAAAAAGAGTTCATGGAAGTTGAAACCCCAATGATGCACGTGATCCCAGGCGGCGCTTCAGCCCGTCCATTTATCA
CTCACCACAATGCGCTGGACATGCCAATGTACCTGCGTATCGCGCCAGAGTTATACCTCAAGCGTTTAGTGGTAGGTGGT
TTTGAGCGTGTGTTCGAAATCAACCGTAACTTCCGTAACGAAGGTCTGTCGCCACGCCATAACCCAGAATTCACTATGAT
GGAATTCTATATGGCCTATGCTGACTACAAAGATCTGATGGACTTAACCGAAGAACTGTTAAGCTCTATCGCGATTGAAC
TGTTAGGCAGCGCGCAAATGCCATACGGCGAGCACACTGTTGATTTCGGTGGCCCATACGCGCGTCTAAGCATGTTAGAA
GCGATTCAGAAGTACAATCCTGACAACGCGACTATCCAAGCAATGACCTACGAGCAAGTGAAAGATCTTGAATTCATGCG
TGAACTGGCTATCAGCCTAGGCATCAAGATCGAGAAGTTCTGGACCTGTGGTCAGCTATTAGAAGAAATCTTCGGTGAAA
CTGCTGAATGGCAGTTAATGCAACCGACTTTCATCACTGGCTACCCAGCGGACATTTCGCCACTGGCACGTCGTAACGAT
GACAATCACTTCATCACCGACCGCTTCGAATTCTTCATCGGTGGCCGTGAAGTGGCTAACGGTTTCAGCGAGTTAAACGA
TGCTGAAGACCAAGACAGCCGCTTTAAAGCGCAGGTTGACGCGAAAGACGCGGGTGACGACGAAGCCATGTTCTACGATG
CAGACTACATCACTGCACTGGAACACGGTTTACCACCAACAGCGGGTCAAGGTATCGGTATCGACCGTTTAGTGATGCTA
TTCACTAACACTCACACTATCCGTGACGTGATCCTGTTCCCAGCGATGCGCCCACAGGCCTAA

Protein sequence :
MTEQVQDENKLIAERRAKLESIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALGFKTSIAGRIMAKRGPFLVIQ
DVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEESRQAFVMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQMPYGEHTVDFGGPYARLSMLE
AIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKFWTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRND
DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-130 57

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_870926.1 lysyl-tRNA synthetase VFG1668 Protein 2e-130 57