Gene Information

Name : Shewana3_2204 (Shewana3_2204)
Accession : YP_869839.1
Strain : Shewanella sp. ANA-3
Genome accession: NC_008577
Putative virulence/resistance : Resistance
Product : Bcr/CflA subfamily drug resistance transporter
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG2814
EC number : -
Position : 2644839 - 2646047 bp
Length : 1209 bp
Strand : -
Note : TIGRFAM: drug resistance transporter, Bcr/CflA subfamily; PFAM: major facilitator superfamily MFS_1; KEGG: sme:SMc00744 putative transport protein transmembrane

DNA sequence :
ATGACAAAATTTAATCAAAACCCGCAAGACCGCAAATGGGATTATACCTTTGCGTACTCACTCCTATTACTTATTCCCTT
CGATTTACTCGCTTCTCTTGGCATGGATCTGTATCTGCCTGCCATCAATGATATTCGCCAGAGTTTTAGTGTTAGCCAAG
GTCAAATGCAGCTGACTTTAACCCTCTATATGGCGATTTTAGGCTTAGGCCAGTTGCTATTTGGACCGCTATCCGACGCA
ATAGGGCGCAGGCGAGTGGTGATTGCCGGAGCCTTAGTTTACGCAGGTTCCAGTTTGGCGATGGCATTGACCGACAGCTT
CAGTGTGTTTCTGCTCCTTCGGATTGTCCAGGCACTGAGCGCCTCGGCCGTGCTAGTGGCGGCATTTGCGACAGTGCGAG
ATGTGTTTGCCGATAGGGAAGAAGGCGTCGTTATCTATGCCATTATGGGCTCAGTGCTCGCGGCCGTGCCTGTGGTAGCA
CCTTTTATTGGCTCGCTTATCGATGGGCTTTGGCATTGGCGAGGGATCTTCTACACCTTAGCGATATTTGCTGCCCTAGT
AGGCTTGCATGCCTTACTGAAATGGCCTGAAACCCGCCCAGCGCAGACCCATCCCTTTGAGTGGTCGAGATGTTTTAACA
TCATTCGCGCTAAGGCGTTTTTAGGCTATACCTTGGCCTACGCCACGGCCATGGGGGCCTTCTTTGTCTATTTTTCGGCA
TCACCGAGCATACTGATGGACAAATTAGGACTCGATAAGGTGACCTTTAGCCTGTGGTTTGGCTCGGTTGCGATAGTCAT
GATAATCACCACCCGCTTTGTTAAGCATCTTGTCAAACGTTGGGGCACACAGGGAACTGTGCTAAGAGGCTTGATTGGCA
TGATGCTAAGTAGCGGCTTATTGTATGGGCTGGAATGGCTAAATGGGCTGAGTTTACTTGGCTTTATGCTGCCTATGTAT
TTCATCGGAGTGCATATCGCCTTAACCTGCGCCGTAACCGCTAATGGTGCCTTAGCGGCGTTTTCCCATTCTGCGGGGCT
GGCAACCGCTCTGTATTATGCCATTGAGAGTCTGTTCTTAAGTGCCTTAGTCAGTCTGTTGATTTGGCTGTTGCCCTCGG
GTACTACGCTGGTGATCTGTAGTTATATTTTGATCTCGGCGTCACTATCACTGCTATTTATTCTCTCCATTAGAGCAGAT
AACAAATAG

Protein sequence :
MTKFNQNPQDRKWDYTFAYSLLLLIPFDLLASLGMDLYLPAINDIRQSFSVSQGQMQLTLTLYMAILGLGQLLFGPLSDA
IGRRRVVIAGALVYAGSSLAMALTDSFSVFLLLRIVQALSASAVLVAAFATVRDVFADREEGVVIYAIMGSVLAAVPVVA
PFIGSLIDGLWHWRGIFYTLAIFAALVGLHALLKWPETRPAQTHPFEWSRCFNIIRAKAFLGYTLAYATAMGAFFVYFSA
SPSILMDKLGLDKVTFSLWFGSVAIVMIITTRFVKHLVKRWGTQGTVLRGLIGMMLSSGLLYGLEWLNGLSLLGFMLPMY
FIGVHIALTCAVTANGALAAFSHSAGLATALYYAIESLFLSALVSLLIWLLPSGTTLVICSYILISASLSLLFILSIRAD
NK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
floR YP_005176183.1 florfenicol / chloramphenicol efflux protein Not tested ICEPmu1 Protein 8e-58 45
flo AAK02049.1 chloramphenicol and florfenicol resistance protein Not tested SGI1 Protein 1e-57 45
floRc AGK06972.1 chloramphenicol and florfenicol resistance protein Not tested SGI1 Protein 1e-57 45
floRc AGK07102.1 chloramphenicol and florfenicol resistance protein Not tested SGI1 Protein 1e-57 45
cml(A)5 ACY75522.1 CmLA(5) Not tested Tn6060 Protein 2e-63 45
cmlA5 CAJ77046.1 Chloramphenicol resistance protein Not tested AbaR1 Protein 3e-63 45
cmlA CAJ77032.1 chloramphenicol and florfenicol resistance protein Not tested AbaR1 Protein 5e-60 44
cmlA9 ABZ01840.1 CmlA9 chloramphenicol efflux protein Not tested SGI2 Protein 5e-60 44

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Shewana3_2204 YP_869839.1 Bcr/CflA subfamily drug resistance transporter DQ522237.1.gene4.p01 Protein 1e-63 45
Shewana3_2204 YP_869839.1 Bcr/CflA subfamily drug resistance transporter NC_010410.6003586.p0 Protein 1e-63 45
Shewana3_2204 YP_869839.1 Bcr/CflA subfamily drug resistance transporter AF294653.1.gene4.p01 Protein 6e-63 45
Shewana3_2204 YP_869839.1 Bcr/CflA subfamily drug resistance transporter DQ984668.gene.p01 Protein 1e-63 45
Shewana3_2204 YP_869839.1 Bcr/CflA subfamily drug resistance transporter AF156486.gene.p01 Protein 2e-61 44