Gene Information

Name : Shewana3_4244 (Shewana3_4244)
Accession : YP_863759.1
Strain :
Genome accession: NC_008573
Putative virulence/resistance : Unknown
Product : HsdR family type I site-specific deoxyribonuclease
Function : -
COG functional category : V : Defense mechanisms
COG ID : COG0610
EC number : 3.1.21.3
Position : 117132 - 120386 bp
Length : 3255 bp
Strand : +
Note : KEGG: mma:MM0431 type I restriction-modification system restriction subnit; TIGRFAM: type I site-specific deoxyribonuclease, HsdR family; PFAM: type III restriction enzyme, res subunit; protein of unknown function DUF450; SMART: DEAD/DEAH box helicase dom

DNA sequence :
ATGATCACAGAAGACCAACTAGAGCAGCTCTGCCTTGACTGGTTTACAGACCAAGGTTATCTATACAAAAATGGCTACGA
CATCGCCCCTGATGGCGATTCACCAGAGCGTGATGATTACCACCAAGTCGTGCTGAAGCAGCGCTTGCTTGAGCGTTTAG
AGGTATTAAACCCTGAACTACCACAAGAGAGCTTGGATGTGGTGGTGAATACGGTAAGTACGCCAGACACGCCAGTACTG
ATTAAAAGCAACCGCGGTTTCCACAAATACCTGATTGAAGGTGTACCTGTTGAGTACACCGTGTATGAAGACGGGGAAAC
GAAAACCAAGCACACCCACGCACGCTTAATGGATTTTACCAATCCAGATAACAACGAGTTTCTGGTGGTGAATCAGTTCA
CCATTACAGGAAAAAAAGGCAACCGTCGCCCTGATGTAGTGGTGTTCATTAATGGTTTGCCGATTGCGGTTATCGAGCTG
AAAAACCCAGCCGACGAACATGCGGACATTTGGCATGCTTACAACCAACTGCAAACCTACAAAGATGAAATTCCTGATCT
GTTTGTTTTCAACGAATCTTTGGTGGTGAGTGATGGTTGGACGGCGCGTGTTGGCTCTCTCACCGCAAACAAAGAACGTT
TTTTACCATGGAAAACTGTTTCTGGTGAAGACGATAAACCGCTCTGGGATTATCAGCTTGAAACCATGGTTAAGGGTTTC
TTCAAGCCTGATTTACTGCTTGATTACATTCGTTACTTTGTCTTGTTTGAAACTGACAACGACACTGTCATCAAGAAAAT
TGCTGGTTACCACCAGTTCCATGCCGTGCGAGCGGCAGTCGAAGCCACAGTGCGAGCGAAACAGGCCGATGGCGAACTGC
CTTTAGTCGCTGAAAGCATGGGGCAATACCAAGTTCAAGCGACAAAAGGCTTAGATAAAATCAAGCCCGGAAGTGGTAAA
GCGGGTGTGGTTTGGCATACGCAAGGCAGTGGTAAAAGTATCTCTATGGTTTGCTACGCAAGCAAACTATTGCAACAGCC
TGCGATGAACAACCCAACGATTGTGGTGGTGACAGACCGTAATGATCTTGATGGTCAGCTTTTTAATACCTTTGGTATGG
CGCATGAAACGCTTAAACAAGTGCCATCGCAAGCGGACGGGCGTGATAAGCTGCGTGAGCTGCTGCTCTCTCGTCAATCT
GGCGGCATCATCTTTACCACCATTCAAAAATTTGCCCTACTTGCTGAAGAAACAGAGCACCCAGTGTTATCAACGCGTGC
CAACATCGTTGTGGTATCGGATGAAGCGCACCGCAGTCAGTACGGTAACAAATCAAAGTTAGTGGACGTTAAAGACGCTA
ATGGCAATGTCATCTCACAAAAGTTTGTTTATGGTTACTCCAAATACATGCGTGATGCGCTGCCAAATGCATCGTTCATT
GGCTTTACGGGAACACCCATTGCCATGGACGACAAAGACACTCGTGGCGTATTTGGTGAGTACGTTTCTATCTATGATAT
TCAAGATGCGGTCGATGATGGTGCTACGGTGCCAATCTACTATGAGTCTCGCTTAGCCAAGCTTGATATCAACCAAGCTG
AAATCGAGCAGCTTAATGACCAAATCGAAGATGAGATCGGTGAGGATGAAGAGACTGCGAGCCGTGAGAAAATCAAATCT
CAATGGGCAACGCTTGAAAAGCTCGTCGGTGCTGAGCCACGTATCCAACAAGTAGCTCAGGACTTAGTGAATCACTTTAC
CACGCGTACCGAAACGTTTCCCGGCAAAGCCATGATTGTGGCGATGAGTCGTGAAATCTGTGTCGATCTCTACAATGCGA
TTGTTGCTATCAAGCCAGAGTGGCACAACGATGACCCTGCTAAAGGGGCTATCAAGATAGTCATGACAGGAAGCGCATCT
GATAAACCTAAGATGCAGCCGCACATCCATGATAAGAAAACCAAGAAGCTCTTTGAGGCGCGATATAAAGATACTCAAGA
TGAACTTCAATTGGTGATTGTTCGTGATATGTGGCTAACAGGCTTTGATGCGCCGTGTTGCCATACCATGTATGTCGATA
AGCCAATGAAAGGTCACAACCTGATGCAGGCTATCGCACGCGTAAACCGCGTTTTTAAAGACAAACCGGGCGGTCTGGTG
GTTGACTATATCGGCATTGCTAATGAGCTTAAAAACGCACTCAAAACCTACACCAATAGCCAAGGTAAAGGGCAACCAAC
CGTCGATACTGCTGAAGCCTTTTCGGTACTGATGGAAAAGGTTGATATCGTTCGTGGCATGTTTGCGACTCCAGTTGATG
GCAAGGTTTTTAACTATCGTCCTGACTTTGAAACTGATGCGCTGCGTTTACTGCCCGGTGCGGTAAACCATCTATCAGGT
TTGTCACACACAGATAGCAAAGGCAAAGAAGTACGCGATGGCAAACGTCGATTCCTCGATGTGATGGCTGCGTTAGGTAA
AGCGTATTCATTGTGTAATACCATGGATGAAACGCGTGGTTACAAGAACGAAATTGCCTTCTACTCTGCGATTAAAGCCG
CGTTTATGAAGCACTCTACCGTTGATAAAAAGCGTACGGATGAAGAGCGCAATACGGCTTTGAAGCAGATCCTCGACAAT
GCTGTGATCGCTGATGGTGTTGATGATATTTTCTCAATGGTTGGTTTAGATAAGCCTAACATCGGTTTGCTTTCTGAAGA
GTTCTTAGAAGACGTTAAGAACATGAAAGAAAAGAACCTTGCTGTTGAGCTGCTTGAGAAGCTGCTGCGTGATGAAGTCA
AAGCTCGGATGAAGAACGATGTGGTGCAAGAGAAAAAATACTCAGACCGCATTTTGACGACATTAAACAAGTACCACAAC
CGTAGCATCGAAACAGCTCAGGTCATTGAAGAGCTAATTCAATGGGCAAAAGAGATGCAAGAAGATGCGGAGATGGTAGA
TAAGCTCAACCTTTCTGTGGATGAGATGGCGTTCTACCGTGCTTTGGTTGAAAACGAGTCCTCAGTTCGGGAGCTTGGCA
ACGATGATTTACGCAACTTAGCGATTGAACTTACTCAGCAACTTCGTAAATCTGCAACGGTTGACTGGCAAAAGCGCGAA
AGCGTTCGCGCTCGTATGCGTAACCTAGTACGCCGACTGCTGCGTCGATGGAAATATCCACCTGATTCCGCAGAGGAAGC
CATCAAGCTTGTGTTAGAACAAGCAGAGGTTCTAGCTGACGGCTGGTATAAGTAA

Protein sequence :
MITEDQLEQLCLDWFTDQGYLYKNGYDIAPDGDSPERDDYHQVVLKQRLLERLEVLNPELPQESLDVVVNTVSTPDTPVL
IKSNRGFHKYLIEGVPVEYTVYEDGETKTKHTHARLMDFTNPDNNEFLVVNQFTITGKKGNRRPDVVVFINGLPIAVIEL
KNPADEHADIWHAYNQLQTYKDEIPDLFVFNESLVVSDGWTARVGSLTANKERFLPWKTVSGEDDKPLWDYQLETMVKGF
FKPDLLLDYIRYFVLFETDNDTVIKKIAGYHQFHAVRAAVEATVRAKQADGELPLVAESMGQYQVQATKGLDKIKPGSGK
AGVVWHTQGSGKSISMVCYASKLLQQPAMNNPTIVVVTDRNDLDGQLFNTFGMAHETLKQVPSQADGRDKLRELLLSRQS
GGIIFTTIQKFALLAEETEHPVLSTRANIVVVSDEAHRSQYGNKSKLVDVKDANGNVISQKFVYGYSKYMRDALPNASFI
GFTGTPIAMDDKDTRGVFGEYVSIYDIQDAVDDGATVPIYYESRLAKLDINQAEIEQLNDQIEDEIGEDEETASREKIKS
QWATLEKLVGAEPRIQQVAQDLVNHFTTRTETFPGKAMIVAMSREICVDLYNAIVAIKPEWHNDDPAKGAIKIVMTGSAS
DKPKMQPHIHDKKTKKLFEARYKDTQDELQLVIVRDMWLTGFDAPCCHTMYVDKPMKGHNLMQAIARVNRVFKDKPGGLV
VDYIGIANELKNALKTYTNSQGKGQPTVDTAEAFSVLMEKVDIVRGMFATPVDGKVFNYRPDFETDALRLLPGAVNHLSG
LSHTDSKGKEVRDGKRRFLDVMAALGKAYSLCNTMDETRGYKNEIAFYSAIKAAFMKHSTVDKKRTDEERNTALKQILDN
AVIADGVDDIFSMVGLDKPNIGLLSEEFLEDVKNMKEKNLAVELLEKLLRDEVKARMKNDVVQEKKYSDRILTTLNKYHN
RSIETAQVIEELIQWAKEMQEDAEMVDKLNLSVDEMAFYRALVENESSVRELGNDDLRNLAIELTQQLRKSATVDWQKRE
SVRARMRNLVRRLLRRWKYPPDSAEEAIKLVLEQAEVLADGWYK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
api52 CAF28526.1 hsdr-like Type I restriction enzyme Not tested YAPI Protein 0.0 61
hsdR YP_251977.1 type I restriction-modification system restriction subunit Not tested SCCmec Protein 0.0 45
hsdR BAH57699.1 type I restriction-modification system endonuclease homologue Not tested Type-VII SCCmec Protein 0.0 45
hsdR BAD24840.1 type I restriction-modification system endonuclease homologue Not tested Type-V SCCmec Protein 0.0 45