Gene Information

Name : lysS (lwe0192)
Accession : YP_848393.1
Strain : Listeria welshimeri SLCC5334
Genome accession: NC_008555
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 191946 - 193442 bp
Length : 1497 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAGTAACGAGAATCATGAAGAACTAAATGACCAACTCATCGTCCGTCGGGAAAAAGTGGACACATTGCGTGAAGAAGG
CATAGATCCTTTTGGTGAAAAATTTATCCGTTCCATCAGCCCAGAAGAAATTGAAACAAAATTCGCTGATAAATCAAAAG
AAGAACTTGAGGAAGCTGCTATCGAAGTTTCTGTAGCAGGTCGTATTATGACGAAGCGTGTAAAAGGTAAAGTAGGTTTC
ACGCATATTCAAGACCGTTTCCATCAATTACAAATCTATATTCGTAAAGACGCTATTGGGGAAGATGCATATGCGATTTT
CAAATTAGCTGATTTAGGAGACATTATTGGCATAAAGGGAACTATTTTCCGTACTAATACAGGAGAATTATCAGTAAAAG
CGACTGATTTTACTTTACTTTCAAAATCATTACGCCCGCTTCCTGATAAATATCATGGCTTAAAAGACGTAGAACAGCGC
TACCGCCAACGCTATTTAGACTTAATTACAAATGAAGAAAGTCAAAATCGTTTTGTTATGCGTAGTAAAATTTTGAAATA
TACACGTGATTACATGGACAGCCAAGGTTTCTTAGAAGTAGAAACTCCAGTGCTCCATACAATTGCTGGTGGCGCTGCAG
CAAAACCATTTATAACGCACCATAATGCACTTGATATGGAACTATACTTACGAATCGCATTGGAACTACATTTGAAACGT
CTAATCGTTGGCGGGATGGATAAGGTATATGAGATTGGTCGTGTCTTCCGTAATGAAGGAACGTCCACGCGACATAACCC
TGAATTTACAATGTTAGAATCATATGCGGCTTACGAAGACTATGAAGATGTTATGGATTTAGTAGAAGGTTTAGTATCAA
CTGTTTGTAAACAAGTAAATGGAACAACAGAAATAACATATGGCGAATACAAAGTAGATTTGACTCCGAATTGGCGCCGC
GTTCACATGGCAGATGTTGTTAAAGAATACGTAGGTGTAGACTTCTGGAATGTGACTTCCGATGAAGAAGCGCGCGAACT
TGCTAAGAAACACGATGTTGCTATAACTGAACACATGACTTATGGTCACATTCTTAACGAGTTTTTCGAAACATACGTAG
AAGAAAAACTTATTCAACCTACATTTGTGTATGGTCACCCAGTAGAAATTTCTCCTCTAGCGAAGAAAAACAAAGAGGAT
GAGCGATTTACTGACCGGTTTGAATTATTCATTGTTGGCCGTGAACACGCTAATGCATTCTCAGAGTTAAATGACCCAAT
TGATCAAAGAGAACGTTTTGAAGCGCAAATGAAAGAACGTGAACAAGGAAATGATGAAGCGCACGGAATGGATGCGGATT
TCCTTGAAGCTTTAGAATATGGTCTGCCTCCAACAGGTGGATTAGGTATAGGTGTAGACCGTCTAGTAATGTTATTAACA
GATGCGCCGTCTATCCGTGATATCCTATTATTCCCAACCATGAAACACCGTGACTAA

Protein sequence :
MSNENHEELNDQLIVRREKVDTLREEGIDPFGEKFIRSISPEEIETKFADKSKEELEEAAIEVSVAGRIMTKRVKGKVGF
THIQDRFHQLQIYIRKDAIGEDAYAIFKLADLGDIIGIKGTIFRTNTGELSVKATDFTLLSKSLRPLPDKYHGLKDVEQR
YRQRYLDLITNEESQNRFVMRSKILKYTRDYMDSQGFLEVETPVLHTIAGGAAAKPFITHHNALDMELYLRIALELHLKR
LIVGGMDKVYEIGRVFRNEGTSTRHNPEFTMLESYAAYEDYEDVMDLVEGLVSTVCKQVNGTTEITYGEYKVDLTPNWRR
VHMADVVKEYVGVDFWNVTSDEEARELAKKHDVAITEHMTYGHILNEFFETYVEEKLIQPTFVYGHPVEISPLAKKNKED
ERFTDRFELFIVGREHANAFSELNDPIDQRERFEAQMKEREQGNDEAHGMDADFLEALEYGLPPTGGLGIGVDRLVMLLT
DAPSIRDILLFPTMKHRD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-112 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_848393.1 lysyl-tRNA synthetase VFG1668 Protein 2e-112 47