Gene Information

Name : lysS (LGAS_0280)
Accession : YP_814126.1
Strain : Lactobacillus gasseri ATCC 33323
Genome accession: NC_008530
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 316838 - 318352 bp
Length : 1515 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
GTGGCAAAGAATGAAATGAACGACCAACTAATTGTTCGTCGTGAAAAAATGGACGAAATGCGTGAAAATGGCATTGAACC
TTTTGGTGTAAGAAAGTTCGATCGTCAAGATTTAGCTCGTACTTTAAATGAAAAGTATAGTAAAGAAGATAAAGATGAAT
TAAATGCAGATATGCCTACGACCAAAATTGCAGGTCGTATGCTTGCAAAACGTGGTAAGGGTAAAGTAGGTTTTGCTGAT
CTTTATGACCGTACTGGTAAGATTCAAATCTATGTACGTAAAGATATTGTTGGCGAAGACAACTACAAGATTTTTAAGAA
ATCAGATATTGGTGACTTCTTAGGTATTGATGGCGAAGTAATGAAAACTGATACTGGTGAGTTAACTATTCGTGCAACTC
ACGTTACTTTCTTATCTAAGGCTCTTCGTCCCTTACCTAATAAATGGGATGGTTTAAAAGATGTTGAACAAATCTATCGT
CAACGCTATCTTGATTTGATTACTAATCACGAAAGTTACATGCGTTTTGTTCATCGTACTCAAATTATTCAAGCTATTCG
TAACTACTTAAATAAGCAAGACTTCTTGGAAGTTGAAACTCCAGTTCTTCACAATATTCCAGGTGGTGCTGAAGCACGTC
CATTTATTACTCACCACAACGCTTTAGATATTGATCTTTATATGAGAATCGCTTTGGAACTTCCATTGAAGCGTTTGATT
GTCGGTGGTATGGAGAGAGTTTATGAAATTGGTCGAGTATTCAGAAACGAAGGTGTTGATACTCGCCACAATCCTGAATT
TACTGAGCTTGAAACTTATGCTGCTTACTGGGATTTCCACGATGTCATGGATGAAGCAGAAGAAATTATCAGAGCAGCTG
CTAAAGTTGTTTCTCCAGACGGTAAGATTAACTACCAAGGTACTGATATTGACTTAGGTAAGCCATTCCGCCGTGTTCAT
ATGGTTGACCTAATTAAGGAAAAGACTGGTGTCGATTTCTGGAAAGAAATGACTGACGAAGAAGCTAAGAAGATCGCCGA
AGAACATGGAATTCATACTGAACCATTTTGGAAAGTTGGTCATGTAATTAATGCTTTCTTTGATGAATTTTGTGAGAAGA
CTATTGTTGATCCAACCTTTGTGTATGGTCACCCTGTAGAAATTTCACCACTTGCTAAAAAGAATGCAGATGATCCAAGA
TTTACTGATCGTTTCGAAATCTTTATTTTAGGAATGGAATACGGAAATGCCTTTTCAGAGTTAAATGATCCAGTTGATCA
ACGTCACCGTTTTGAAGAACAAATGGAAGAACGCGAAGCTGGTAACGATGAAGCTGATATGATTGATGAAGATTATATCC
GTGCTATGGAATTCGGTATGCCTCCTACAGGTGGTTTAGGTATCGGTATCGACCGTTTGGTAATGCTTTTAACTGACGCT
CCAGCTATCCGTGATGTATTGCTCTTCCCAACAATGCGTCCTGAAAAGGTTGAAGATGTTGATGCAGAAGTATAA

Protein sequence :
MAKNEMNDQLIVRREKMDEMRENGIEPFGVRKFDRQDLARTLNEKYSKEDKDELNADMPTTKIAGRMLAKRGKGKVGFAD
LYDRTGKIQIYVRKDIVGEDNYKIFKKSDIGDFLGIDGEVMKTDTGELTIRATHVTFLSKALRPLPNKWDGLKDVEQIYR
QRYLDLITNHESYMRFVHRTQIIQAIRNYLNKQDFLEVETPVLHNIPGGAEARPFITHHNALDIDLYMRIALELPLKRLI
VGGMERVYEIGRVFRNEGVDTRHNPEFTELETYAAYWDFHDVMDEAEEIIRAAAKVVSPDGKINYQGTDIDLGKPFRRVH
MVDLIKEKTGVDFWKEMTDEEAKKIAEEHGIHTEPFWKVGHVINAFFDEFCEKTIVDPTFVYGHPVEISPLAKKNADDPR
FTDRFEIFILGMEYGNAFSELNDPVDQRHRFEEQMEEREAGNDEADMIDEDYIRAMEFGMPPTGGLGIGIDRLVMLLTDA
PAIRDVLLFPTMRPEKVEDVDAEV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-110 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_814126.1 lysyl-tRNA synthetase VFG1668 Protein 7e-111 47