Gene Information

Name : lysS (LBUL_0328)
Accession : YP_812428.1
Strain : Lactobacillus delbrueckii ATCC BAA-365
Genome accession: NC_008529
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 319386 - 320891 bp
Length : 1506 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
TTGGCAAAGAACGAAATTAACGACCAATTGCTTGTTCGTCGGCAAAAGCTTGAAGAACTTAAGGAGATGGGGATTGAACC
CTTCGGCCGCAAATATGATCGGGAAGCCCTGGCCCGCGACCTGGACGAAAAGTACTTGGGCCTTGACAAGGATGAACTGA
ACGCAAATATGCCGAAGACCAAGATCGCTGGCCGGATGCTGGCCAAGCGGGGCAAGGGCAAGGTTGGCTTTGCGGACCTC
TATGACCGTAGCGGCAAGATCCAGATCTACGTCCGCAAGGACATGGTCGGTGAAGAAAACTACCAGATTTTCAAGAAGGC
CGACCTTGGTGACTTCCTGGGCGTTGAAGGCGAAGTCTTTAAGACTGACTCAGGCGAATTGTCTGTCCGGGCCCAGCACG
TGACCTTCCTGTCCAAGGCTTTGCGGCCACTGCCTGACAAGTACCACGGTCTTAAGGACGTTGAATCCATTTATCGCCAA
CGCTACCTGGACTTGATCACCAACCACGAAAGCTACCAGCGCTTCGTTAACCGGACCAAGATCATCCAGGCCATCCGTGA
CTACTTGAACGGGCAGGACTTCCTGGAAGTTGAAACGCCAGTTTTGCACAACATCCCAGGCGGGGCTGAAGCACGTCCGT
TCATCACCCACCACAACGCTTTGGACATCAACCTCTACATGCGGATTGCCCTGGAATTGCCGCTGAAGCGTCTGATCGTC
GGTGACATGGAACGGGTTTACGAAATCGGCCGGGTCTTCAGAAATGAAGGGATGGACCTCAAGCACAACCCTGAATTTAC
TGAACTGGAAACCTACGCTGCTTACTACGACTTCCACGATGTCATGGATGAAGCTGAAGGCATCATCCGGGCAGCGGCCA
AGGTTGTTTCAGAAGATGGCAAGATCAACTACCAGGGCACTGACCTGGACTTGGGCAAGCCATTCCGCCGGGTGCACATG
GTTGACCTGATCAAGGAACTGACTGGCGTTGACTTCTGGCAGCCAATGACTCTGGAAGAAGCCCGGAAGGTTGCCGCTGA
ACACCATGTTGAAGTTGAACCTTACTGGCAAGTTGGCCACATCATCAACGCCTTCTTTGAAAAATTTGGTGAAAAGACAA
TCGTTGACCCAACCTTCGTTTACGGCCACCCAGTTGAAGTTTCACCGCTGGCCAAGAAGAACGCGGATGACCCGCGCTTC
ACTGACCGTTTCGAAATCTTCATCATGGGCAATGAATACGGCAACGCCTTCTCAGAACTGAACGACCCGCTTGACCAGCG
GGAACGGTTCGAAGCCCAAATGGCTGAACGTGACGCCGGTAACGACGAAGCCGACATCATCGACGAAGACTACCTGCGGG
CCATGGAATACGGTATGCCACCTACGGGTGGTCTGGGCATCGGGATCGACCGTCTGGTCATGCTTTTGACCAACGCGGCT
TCAATCCGCGACGTCCTCCTCTTCCCAACCATGCGGCCGGAAAAGAACATTAACCTGGACAAGTAG

Protein sequence :
MAKNEINDQLLVRRQKLEELKEMGIEPFGRKYDREALARDLDEKYLGLDKDELNANMPKTKIAGRMLAKRGKGKVGFADL
YDRSGKIQIYVRKDMVGEENYQIFKKADLGDFLGVEGEVFKTDSGELSVRAQHVTFLSKALRPLPDKYHGLKDVESIYRQ
RYLDLITNHESYQRFVNRTKIIQAIRDYLNGQDFLEVETPVLHNIPGGAEARPFITHHNALDINLYMRIALELPLKRLIV
GDMERVYEIGRVFRNEGMDLKHNPEFTELETYAAYYDFHDVMDEAEGIIRAAAKVVSEDGKINYQGTDLDLGKPFRRVHM
VDLIKELTGVDFWQPMTLEEARKVAAEHHVEVEPYWQVGHIINAFFEKFGEKTIVDPTFVYGHPVEVSPLAKKNADDPRF
TDRFEIFIMGNEYGNAFSELNDPLDQRERFEAQMAERDAGNDEADIIDEDYLRAMEYGMPPTGGLGIGIDRLVMLLTNAA
SIRDVLLFPTMRPEKNINLDK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 4e-112 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_812428.1 lysyl-tRNA synthetase VFG1668 Protein 2e-112 47