Gene Information

Name : lysS (LACR_0417)
Accession : YP_808446.1
Strain : Lactococcus lactis SK11
Genome accession: NC_008527
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 388168 - 389652 bp
Length : 1485 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
TTGGCTGAAATCGAAGAACTTAATGACCAAATGAAAGTCCGCCGTGAAAAAATGGGAAACCTGCACGATGCAGGAATTGA
CCCATTTGGACATAAATTTACACGTACGCACAACTCGCAAGAACTACACGAAGCTTATGACGAAAAGACGAAAGAAGAGT
TGCATGAACTTGCACTTTCAGGAATTGTCGCTGGTCGTTTGATGACTAAACGTGGTAAAGGTAAAGTCGGTTTCGCCCAT
CTTCAAGACCGCGAAGGCCAAATTCAACTTTACGTTCGTAAAGACGAAGTTGGTGAAGAAAATTATGAAATTTTCAAAAA
AGCTGACCTCGGAGACTTCCTCGGAGTTGAAGGCGAAATCATGAAAACTGATATGGGTGAATTGTCAATTAAAGCAAAAA
AATTGACTTTCCTCTCAAAAGCGCTTCGTCCTTTGCCTGAAAAATTCCATGGTTTGACTGATACTGAAACACGTTATCGC
AAACGTTATTTGGATTTAATTTCAAATAAAGAAAGCTTTAATCGCTTTGTGACTCGCTCAAAAATTATCAGTGAAATCCG
TCGTTATATGGATGGTCGTGGATATCTTGAAGTTGAAACTCCAGTTCTTAACAACGAAGCTGGTGGTGCTTCTGCTCGCC
CATTCTACACTCACCATAATAGCCTTGATATTGACATGGCGCTTCGTATTGCAACTGAATTGCACTTGAAACGTTTGATT
GTTGGTGGAATGGAAAAAGTTTATGAACTTGGTCGTGTCTTCCGTAATGAAGGAATGGATATGACTCATAATCCTGAATT
CACAACAATGGAATCTTATGAAGCTTATGCTGATTTTGAAGATATCATGGATTTGACAGAAGGAATCTTCCAACACGTTG
CTAAAACTGTAGTTGGTCAAGATGTTCTTGAATACGATGGTAAAGAAATCAATGTTGGTGGAAAATTCAAACGTGTTCAC
ATGGTTGATGCCATCAAGGAAGTTGCTGGAGTTGATTTCTGGCCTGAGATGACTTTTGAAGAAGCAACAGCTCTTGCAAA
AGAACATGATATTCATGTTGAAAAACATTTCACTTCTGTTGGTCACATCATTAACGAATTCTTTGAAAAATATGTTGAAG
AAACTTTGATTCAACCTACTTTCGTTTTTGGTCATCCAAAAGAAATTTCACCACTTGCTAAAATGAACGAGAAAGACCCA
CGTTTTACTGACCGTTTTGAACTTTTCATCAACGGTAAAGAATACGCCAATGCTTTCAGCGAATTGAATGACCCAATTGA
CCAATTGGAACGTTTTGAAGCTCAAGCCAAAGCTAAAGAACTTGGGGATGATGAAGCGACTGGTGTCGATTACGATTACG
TTGAAGCACTCGAACACGGTATGCCACCAACAGGTGGACTCGGTATCGGGATTGACCGCTTAGTCATGCTCTTTACTGGC
ACAACTTCAATCCGTGACGTTTTGCTTTTCCCAACGATGAAATAA

Protein sequence :
MAEIEELNDQMKVRREKMGNLHDAGIDPFGHKFTRTHNSQELHEAYDEKTKEELHELALSGIVAGRLMTKRGKGKVGFAH
LQDREGQIQLYVRKDEVGEENYEIFKKADLGDFLGVEGEIMKTDMGELSIKAKKLTFLSKALRPLPEKFHGLTDTETRYR
KRYLDLISNKESFNRFVTRSKIISEIRRYMDGRGYLEVETPVLNNEAGGASARPFYTHHNSLDIDMALRIATELHLKRLI
VGGMEKVYELGRVFRNEGMDMTHNPEFTTMESYEAYADFEDIMDLTEGIFQHVAKTVVGQDVLEYDGKEINVGGKFKRVH
MVDAIKEVAGVDFWPEMTFEEATALAKEHDIHVEKHFTSVGHIINEFFEKYVEETLIQPTFVFGHPKEISPLAKMNEKDP
RFTDRFELFINGKEYANAFSELNDPIDQLERFEAQAKAKELGDDEATGVDYDYVEALEHGMPPTGGLGIGIDRLVMLFTG
TTSIRDVLLFPTMK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-113 49

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_808446.1 lysyl-tRNA synthetase VFG1668 Protein 8e-114 49