Gene Information

Name : pykA (PA14_56240)
Accession : YP_792674.1
Strain : Pseudomonas aeruginosa UCBPP-PA14
Genome accession: NC_008463
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 5020400 - 5021851 bp
Length : 1452 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGTCCGTTCGCCGCACCAAAATCGTCGCCACCCTCGGCCCAGCCAGCAATTCCCCGGAAGTGCTGGAGCAACTGATCCT
GGCCGGGATCGACGTCGCCCGCCTCAACTTCTCCCACGGCACCCCCGATGAACACCGCGCCCGCGCCAGGCTGGTGCGCG
AACTGGCGGCCAAGCACGGCCGCTTCGTCGCCCTGCTCGGCGACCTGCAAGGACCGAAGATCCGCATCGCCAAGTTCGCC
AACAAGCGCATCGAATTGCAGGTCGGCGACAAGTTCCGCTTCTCCACCAGCCACGCCCGCGACGCCGGTACCCAGGAAGT
GGTCGGCATCGACTACCCGGACCTGGTGAAGGACTGCGGGGTCGGCGATGAACTGCTGCTGGACGACGGCAGGGTGGTGA
TGGTGGTCGAGGAAGTCGCCGCCGACGAACTGCGCTGCCGGGTGCTGATCGGCGGCCCGCTGTCCGACCACAAGGGCATC
AACCGCCGCGGCGGCGGTCTCACCGCGCCGGCGCTGACCGACAAGGACAAGGCCGACATCAAGCTGGCCGCGGACATGGA
CCTGGACTACGTGGCCGTTTCCTTCCCGCGCGACGCCAAGGACATGGAATACGCCCGCCGCCTGCTCACCGAGGCCGGAG
GCAAGGCCTGGCTGGTAGCCAAGATCGAGCGCGCCGAGGCGGTCGCCGACGACGACGCCCTCGACGGCCTGATTCGTGCC
AGCGACGCGGTGATGGTGGCCCGTGGCGACCTCGGCGTGGAAATCGGCGATGCCGAGCTGGTCGGCATCCAGAAGAAGAT
CATCCTGCACGCCCGCCGCAACAATAAAGTGGTGATCACCGCGACCCAGATGATGGAGTCGATGATCCACAGCCCGATGC
CGACCCGCGCGGAAGTCTCCGACGTGGCCAACGCCGTGCTCGACTACACCGACGCGGTGATGCTCTCGGCGGAAAGCGCC
GCCGGCGAGTACCCGGTGGAAGCGGTGCAAGCCATGGCCCGCGTCTGCCAGGGCGCCGAGAAGCACCCGACCAGCCAGAA
ATCCAGCCACCGCCTCGGCCAGACTTTCGACCGCTGCGACGAGAGCATCGCCCTGGCGTCGATGTACACGGCCAACCACT
TCCCGGGGATCAAGGCGATCATCTGCCTGACCGAAAGCGGCTTCACCCCGCTGATCATGTCGCGCATCCGCTCGTCGGTA
CCGATCTACGCCTACTCCCCGCACCGCGAGACCCAGGCTCGCGTGGCGATGTTCCGCGGCGTGGAAACCATCCCCTTCGA
CCCGGCGGCGCTGCCGGCGGAGAAGGTCAGCCAGGCCGCGGTCGACGAGTTGCTCAAGCGCGGCGTGGTGACCAAGGGCG
ACTGGGTGATCCTGACCAAGGGCGACAGCTACACCGCCCAGGGCGGCACCAACACCATGAAGGTGCTGCACGTCGGCGAC
CTGCTGGTATAA

Protein sequence :
MSVRRTKIVATLGPASNSPEVLEQLILAGIDVARLNFSHGTPDEHRARARLVRELAAKHGRFVALLGDLQGPKIRIAKFA
NKRIELQVGDKFRFSTSHARDAGTQEVVGIDYPDLVKDCGVGDELLLDDGRVVMVVEEVAADELRCRVLIGGPLSDHKGI
NRRGGGLTAPALTDKDKADIKLAADMDLDYVAVSFPRDAKDMEYARRLLTEAGGKAWLVAKIERAEAVADDDALDGLIRA
SDAVMVARGDLGVEIGDAELVGIQKKIILHARRNNKVVITATQMMESMIHSPMPTRAEVSDVANAVLDYTDAVMLSAESA
AGEYPVEAVQAMARVCQGAEKHPTSQKSSHRLGQTFDRCDESIALASMYTANHFPGIKAIICLTESGFTPLIMSRIRSSV
PIYAYSPHRETQARVAMFRGVETIPFDPAALPAEKVSQAAVDELLKRGVVTKGDWVILTKGDSYTAQGGTNTMKVLHVGD
LLV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 4e-64 45
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 7e-49 43
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 7e-49 43
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 7e-49 43
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 4e-48 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA YP_792674.1 pyruvate kinase VFG0479 Protein 3e-49 43