Gene Information

Name : lysS (Sfri_0583)
Accession : YP_749282.1
Strain : Shewanella frigidimarina NCIMB 400
Genome accession: NC_008345
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 677097 - 678599 bp
Length : 1503 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACTGAACACGTACAAGATGAAAACAAACTGATTGCTGAACGTCGCGCTAAATTAGAACACATTCGCACCAACTGTCC
TGCGAATGCTCATCCAAATACATGGCAGCGTAGTCATAAAGCGGCTGAGTTACAGGCACAATATGGCGAGCAAACTAAAG
AAGCGCTAGAAGAGTTAGCTTTTCAAACCAGTATTGCTGGCCGTGTTATGGCAAAGCGTGGTCCATTTTTAGTGATTCAA
GACGTGTCTGGTCGTATTCAAGCTTATGCTGGTAAGCCAGTACAAGGCGATTTAAAAGAGCGTTATCAAGGTTTAGACAT
CGGTGACATCATTGGTGTTAAAGGTCAGCTGCATTTATCTGGCAAGGGCGACTTATACGTTAATATGGAAGAATATCAGT
TACTGACCAAAGCATTGCGTCCGTTACCTGAAAAATTCCATGGTTTAACAGACCAAGAAACTCGCTACCGTCAGCGTTAC
GTTGATTTGATCGTTAACGAAGATTCGCGCAATGCCTTTATTATGCGCTCTAAAGTGGTTGCCGCAATCCGTAACTTTAT
GGTCAAAAAAGAGTTCATGGAAGTTGAAACCCCAATGATGCACGTTATTCCTGGTGGTGCTTCTGCGCGTCCATTTGTAA
CGCATCACAATGCATTAGACATGGCTATGTACTTACGTATCGCGCCAGAGCTGTACCTTAAGCGTCTAGTGGTTGGTGGT
TTTGAACGTGTATTCGAAATTAACCGTAACTTCCGTAACGAAGGTTTGTCACCACGTCATAACCCAGAATTCACCATGAT
GGAATTCTATATGGCGTATGCTGATTACAAAGACCTAATGGACTTAACCGAAGAAATGCTGGGGTCAATTGCTCAAGAAT
TATTAGGCAGTACTAGCATGCCGTATGGTGAAGAAACTGTTGAGTTTGGTGGCGCTTATGCTCGTTTAAGCATGTTAGAA
GCGATTCAGAAATACAATCCTGACAATGCGACTATTCAAGCTATGACCTACGAGCAAGTTAAAGACGTAGAGTTTATGCG
CGACTTAGCATCGAGCCTAGGCATCAAGTTAGAGAAATTCTGGACTTGTGGTCAATTACTTGAAGAAATCTTCGGTGAAA
CTGCTGAAACTAAGTTAATGCAACCTACGTTCATTACTGGCTACCCAGCTGATATTTCACCTTTGGCGCGTCGTAATGAT
AACAATGACTTCATTACCGATCGTTTTGAGTTCTTTATCGGTGGCCGCGAAGTGGCTAACGGCTTTAGTGAGCTTAACGA
TGCTGAAGATCAAGACAACCGCTTTAAAGCGCAAGTTGATGCGAAAGATGCTGGCGATGACGAGGCGATGTTCTATGACG
CTGACTACATTACAGCACTAGAGCACGGTTTACCACCAACAGCAGGTCAAGGTATTGGTATCGACCGTTTAGTGATGCTA
TTCACTAACACGCATACTATTCGCGACGTGATTTTATTCCCAGCGATGCGCCCACAAGCGTAA

Protein sequence :
MTEHVQDENKLIAERRAKLEHIRTNCPANAHPNTWQRSHKAAELQAQYGEQTKEALEELAFQTSIAGRVMAKRGPFLVIQ
DVSGRIQAYAGKPVQGDLKERYQGLDIGDIIGVKGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEDSRNAFIMRSKVVAAIRNFMVKKEFMEVETPMMHVIPGGASARPFVTHHNALDMAMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEEMLGSIAQELLGSTSMPYGEETVEFGGAYARLSMLE
AIQKYNPDNATIQAMTYEQVKDVEFMRDLASSLGIKLEKFWTCGQLLEEIFGETAETKLMQPTFITGYPADISPLARRND
NNDFITDRFEFFIGGREVANGFSELNDAEDQDNRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-136 57

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_749282.1 lysyl-tRNA synthetase VFG1668 Protein 7e-137 57