Gene Information

Name : Mlg_0731 (Mlg_0731)
Accession : YP_741575.1
Strain : Alkalilimnicola ehrlichii MLHE-1
Genome accession: NC_008340
Putative virulence/resistance : Resistance
Product : acriflavin resistance protein
Function : -
COG functional category : V : Defense mechanisms
COG ID : COG0841
EC number : -
Position : 813396 - 816437 bp
Length : 3042 bp
Strand : +
Note : PFAM: acriflavin resistance protein; KEGG: dde:Dde_0670 transporter, AcrB/D/F family

DNA sequence :
ATGAACCTGGCCGCGCTGGCCATACGCCACCGCATCACCACCCTGGTGGCCGTCCTGCTGCTGGTGGTCATGGGCTGGCA
GGCCTACCACAACGTCGGCTGGTTGGAGGACCCGGAGTTCAGCATCAAGCTGGCGCTGGTGGTCACCCAGTACCCGGGTG
CCTCACCGGAAGAGGTGGAGCTGCAGGTCACCGACCCCCTGGAACGGGCCATCCAGGACCTGGATGAGCTGGACCATGTG
CGCAGCCTCTCCCGCGCCGGTGAGTCCGTCATCTATGCCGAGATCCAGCAGCGCTACCGGGCCGACGACCTGCCGCAGAT
CTGGGACCGCCTGCGGCAAAAGGTCGGCGACGCCCAGGCGCAGCTGCCCAGCGGCGCCCACCCATCGCGGGTGATCGACG
ATTTCGGTGATGTCCACAGCATCGTGCTTGCCGTCCACGGCGCGGGGGTGGAGCGCGCCCGCCTGGAGGACTATGCCGAC
GAACTCAAGCGCGACCTGCTGGGCGTGGACGGCGTCGCCCGCGGCAGTCTCTGGGGGGCGCAGCCCGAGGCGGTCTACGT
GGACCTGTCCGAGTCGCGCATCGCCATGATGGGCTTGGCTGCCGGTCAGGTGGTGCCGTTGCTGTTCGACCAAACCAAGG
TCCAGGACGCTGGCTGGGCGGAGCTGGGCGACCGCCGGCTGCGGGTCATCCCCAGTGGCACCTTCAATACCCTGGACGAG
TTGCGCGACTTCTTGATTCCCGGCGGCGAGTCGGGCGAGTTGGTGCGCCTGGGGGACATCGCCGAGATCAGCCGCGGGCC
GCTGACCCCGCTCCGGCCGCTGATGCGCTTCAACGGCGAACCGGCGGTGGGCATCGCCCTGGCCCACGAGGAGGGCAGCA
ATGTGGTGGCCATGGGGCGCGCCGTGCAGCAGCGACTGGACGAGCTGGCGCCCTTCACTCCGGCGGGAATCGAGCTGGGG
GTGGTCGCCTGGCAGCCGCAGATCGTGGACGATGCGGTCAGCGGCTTCGTTCGCAACGTTCTGATTGCGCTGGTGATTGT
CCTCGGGGTGCTGTGCCTGTTCACCGGCCTGCGCAGCGGCCTGATGGTGGGGGTGGCGCTGCTGCTCACGGTGGCCACCA
CCCTGGTGTTCATGCGGGTGTTCGGTATCGACCTGCACCGGGCCTCGGTGGGGGCGATGATCATCTCCCTGGGGATGATG
GTGGACAATGCCATCGTCACCGCGGATCTCATGCAGGTAAAGATCCGGCGCGGGGTGGAGCGCCTGCAGGCGGCCCGCGA
GGCGGTGCGCGAGAGCGGCCTGCCGCTGCTCGGTGCCACCCTGGTGGGGATCTTCGGCTTTCTGGCGATCTTCCTGGCCG
AGGACGACACCGGCGAGTATGTCGCCAGCCTCTTTCAGGTGGTGGGCATCTCCCTGCTGGTGAGCTGGGTGGCGGCCATG
ACCATCGTCCCGCTGCTGGGCTTCCTGCTGTTCAAACCCGGGCAGCAGACCACCGAGCCCTATCAGACCCGCTTTTACGG
GGCCTACCGGCGGCTGCTGCACGGCGTGCTGCGCCACCGACCGCTGGTGGTCATCACGGTGATCGCCACCGTGGCCCTGG
CCGTCATCGGCTTCCGCTTTGTCGACCAGAGCTTCTTTCCCAACGCCGATCGTAACGAGGTGATGCTCGACTACTGGCTG
CCGGAAGGCGGGCGCATCGAGCAGGTGGCGGCGGACCTGCGCGAGATCGAGGACCACTTCCTGGCCATGCCCGAGGTGGA
GCGGGTGACCAGCTTCATCGGTGAGGGCGCCCCGCGCATTGTACTGGCCATGGAACCGGAGCTGCACAACCAGAGCTACG
GCCAGCTACTCATCCGCCTGCAGGACGCCGGCCAGGTGTCGGAGATCGTTCGCCGTGGCGATGCCTACCTGGCACAGCAT
TTCCCCCAGGCGGAGCCCCGGATGCGCCCCTTCAGCCTGGCCTCGGTGGAGAAGTTCAAGGTCGAGGGCCGGTTCCGCGG
CCCCGACCCGGCGGTCCTCCGGGCGTTGGCCGCTCAGGCGGAGGCGATCCTGCGCGCCGATCCCGAGGCCAAATACGTGC
GCAACGACTGGCGCCAGCCGGTGAAGACCCTGGAGCCGGTCATCGACCAGGCTGCCGCCCGCCGCGCCGGGGTCACCCGT
GAGGCCATCGGTCAGGCCTTCCTGCGCGCTGAGCACGGCCTGGACATGGGGATGTACCGGGAGGGCGACGACCGGCTGCC
CATCCGCCTGCGCCGCCCGCCGGATGAGCGCGAGCGGCTCTCGGACCTGCGCCATATCCAGGTCTGGCCCGCGGATCGCC
CCTGGTCCGTGCCGCTGGACCAGTTGCTGGAGACGCTGCGGGTGGACCGGGAGGACAGCCTGCGCTGGCGGCGGGACCGG
CAGCCCACCCTCACCGTGCAGGCGGATCCGGTGAATATTGAGGCCCACGAGCTCCACGGCCGGGTGCGCGACGCCATCGA
GGCCATCGAGTTGCCGCCCGGCTACCACTTCGAATGGGGCGGGGAGCACGAGGCGGCCCAGCGCAGTGAGGCCGAGGTGT
TGTCCAAGGTCCCGGTGAGTCTGGCGCTGATGGTGCTGATCACGGTGGCCCTGTTCAACGGCTTGCGCCAGCCGCTGATC
ATCTTCATGACCATCCCGCTGGGCATCGTCGGGGTCGCCGCCGGCCTGCTGGTGACCGGACTGCCCTTCGGCTTCATGGC
CCTGCTGGGGCTGATCTCCCTGTCGGGGATGTTGATCAAGAACGGGGTGGTGCTGCTGGATCAGATCGAGCAGTACATCG
CCAGCGGCATGGACCGCTACCAGGCGGTGGTGGAGGCCTCGGTGGGGCGCCTCTCGCCCATCTCCATGACGGCGCTGACC
ACCGCCCTGGGGATGACCCCCATGCTCACCGACACCCTGTTCCGGGTGATGGCGGTGAGCATGATCGGGGGGCTGCTGTT
CGCCACGCTGCTGACCCTTTTCCTGGTGCCGGCGCTCTACACCTGGCTGTTTCGGGTGCGTCCGCCAGAGCCCGCGGAGT
GA

Protein sequence :
MNLAALAIRHRITTLVAVLLLVVMGWQAYHNVGWLEDPEFSIKLALVVTQYPGASPEEVELQVTDPLERAIQDLDELDHV
RSLSRAGESVIYAEIQQRYRADDLPQIWDRLRQKVGDAQAQLPSGAHPSRVIDDFGDVHSIVLAVHGAGVERARLEDYAD
ELKRDLLGVDGVARGSLWGAQPEAVYVDLSESRIAMMGLAAGQVVPLLFDQTKVQDAGWAELGDRRLRVIPSGTFNTLDE
LRDFLIPGGESGELVRLGDIAEISRGPLTPLRPLMRFNGEPAVGIALAHEEGSNVVAMGRAVQQRLDELAPFTPAGIELG
VVAWQPQIVDDAVSGFVRNVLIALVIVLGVLCLFTGLRSGLMVGVALLLTVATTLVFMRVFGIDLHRASVGAMIISLGMM
VDNAIVTADLMQVKIRRGVERLQAAREAVRESGLPLLGATLVGIFGFLAIFLAEDDTGEYVASLFQVVGISLLVSWVAAM
TIVPLLGFLLFKPGQQTTEPYQTRFYGAYRRLLHGVLRHRPLVVITVIATVALAVIGFRFVDQSFFPNADRNEVMLDYWL
PEGGRIEQVAADLREIEDHFLAMPEVERVTSFIGEGAPRIVLAMEPELHNQSYGQLLIRLQDAGQVSEIVRRGDAYLAQH
FPQAEPRMRPFSLASVEKFKVEGRFRGPDPAVLRALAAQAEAILRADPEAKYVRNDWRQPVKTLEPVIDQAAARRAGVTR
EAIGQAFLRAEHGLDMGMYREGDDRLPIRLRRPPDERERLSDLRHIQVWPADRPWSVPLDQLLETLRVDREDSLRWRRDR
QPTLTVQADPVNIEAHELHGRVRDAIEAIELPPGYHFEWGGEHEAAQRSEAEVLSKVPVSLALMVLITVALFNGLRQPLI
IFMTIPLGIVGVAAGLLVTGLPFGFMALLGLISLSGMLIKNGVVLLDQIEQYIASGMDRYQAVVEASVGRLSPISMTALT
TALGMTPMLTDTLFRVMAVSMIGGLLFATLLTLFLVPALYTWLFRVRPPEPAE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
VC0395_A1355 YP_001217299.1 AcrB/AcrD/AcrF family transporter Not tested VPI-2 Protein 4e-144 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Mlg_0731 YP_741575.1 acriflavin resistance protein BAC0519 Protein 2e-154 42
Mlg_0731 YP_741575.1 acriflavin resistance protein BAC0428 Protein 3e-149 42
Mlg_0731 YP_741575.1 acriflavin resistance protein BAC0425 Protein 2e-144 41