Gene Information

Name : pykA (HS_1086)
Accession : YP_719298.1
Strain : Haemophilus somnus 129PT
Genome accession: NC_008309
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1214992 - 1216431 bp
Length : 1440 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGTCTAGAAGACTAAGAAGAACAAAAATAGTATGTACACTGGGACCATCCACAGATCGAGGTAATAATTTAGAAAAAAT
TATTGTAGCCGGTGCAAATGTTGTTCGTATGAATTTTTCTCATGGTACACCAGAAGATCATCTTGCTCGTGCTGAAAAAG
TTCGTGAAATTGCTAAAAAATTAGGTAAAACTGTTGCAATTTTAGGAGATCTACAAGGTCCAAAAATTCGGGTATCTACT
TTTAAAGAGGGTAAAATTTTCTTAAATATTGGTGATAATTTTACTTTAGATGCTGATTTACCCAAAGGTGAAGGTAATCA
AGAAGCCGTAGGACTGGATTATAAAACATTACCGCAAGATGTTGTTCCGGGCGATATTTTATTATTGGATGACGGACGTG
TTCAATTAAAAGTGTTATCGACAGACAATACTAAAGTATTTACAGAAGTAATTGTTGGCGGTCCATTATCAAATAATAAA
GGTATCAATAAATTAGGTGGTGGGCTATCTGCTAACGCACTGACAGAAAAAGACAAAGAAGACATTATTTTAGCTGCACG
TATTGGTGTTGATTATCTTGCTGTTTCTTTCCCTCGTTCCAGTGCTGATTTAAATTATGCTCGTGAATTAGCTCAACAAG
CAGGTTTAAAAGCCAAAATTGTGGCTAAAGTTGAAAGAGCGGAGACTGTTATTGACGAGGAGGCAATGGACGATATTATT
TTAGCTTCTGATGTAATTATGGTTGCTCGTGGTGATTTGGGTGTTGAAATTGGTGATCCGGAATTAGTCGGAATACAGAA
AAAATTAATTCGTCGTTCACGTCAGCTAAATCGTGTAGTGATTACCGCAACACAAATGATGGAATCTATGATTAGCAATC
CTATGCCAACTCGTGCCGAGGTGATGGATGTTGCCAATGCTGTATTAGATGGCACTGATGCCGTCATGCTTTCTGCGGAA
ACAGCAGCAGGTCAATACCCTTCAGAAACTGTTTCAGCAATGGCTAAAGTTTGTTTAGGTGCTGAAAAAATGCCTAGTGT
AAATATTTCTCGGCATCGCTTAGATAAAGAATTTGATACGATTGAAGAAACTATTGCGATGTCTACGATGTTTGCAGCCA
ACCATATGAAAGGTGTTGCAGCCATTATCGCAATGACAAGTAGTGGTCATACTGCAAAATTGATGTCTCGCATTAGTTCC
GGACTACCAATTTTTGCCCTTTCTCGAAATGAAAGTACATTAAATTTATGTGCATTGTATCGTGGTGTTATTCCAATTCA
TTTTGATCAGGATAGTCGTACGCTTGAAGGATTAAAAGCTGCCATACAATCGCTAAAAGATAAAGGTTATTTAGTTTCCG
GTGATTTGGTTTTATTAACTCAAGGAGACCACTTAACATCAAATGGTACGAGTACTTGCCGTGCTTTAGTGGTTGAATGA

Protein sequence :
MSRRLRRTKIVCTLGPSTDRGNNLEKIIVAGANVVRMNFSHGTPEDHLARAEKVREIAKKLGKTVAILGDLQGPKIRVST
FKEGKIFLNIGDNFTLDADLPKGEGNQEAVGLDYKTLPQDVVPGDILLLDDGRVQLKVLSTDNTKVFTEVIVGGPLSNNK
GINKLGGGLSANALTEKDKEDIILAARIGVDYLAVSFPRSSADLNYARELAQQAGLKAKIVAKVERAETVIDEEAMDDII
LASDVIMVARGDLGVEIGDPELVGIQKKLIRRSRQLNRVVITATQMMESMISNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPSETVSAMAKVCLGAEKMPSVNISRHRLDKEFDTIEETIAMSTMFAANHMKGVAAIIAMTSSGHTAKLMSRISS
GLPIFALSRNESTLNLCALYRGVIPIHFDQDSRTLEGLKAAIQSLKDKGYLVSGDLVLLTQGDHLTSNGTSTCRALVVE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 1e-77 49
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-57 41
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-56 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-56 41
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-56 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA YP_719298.1 pyruvate kinase VFG0479 Protein 4e-57 41