Gene Information

Name : lysS (RHA1_ro03733)
Accession : YP_703691.1
Strain : Rhodococcus jostii RHA1
Genome accession: NC_008268
Putative virulence/resistance : Unknown
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3938273 - 3941617 bp
Length : 3345 bp
Strand : -
Note : catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA

DNA sequence :
ATGTCAGCTAGCACCGAGACACACCACGCGTCCGAGGCGGCCGTCCCCACCGCACCACGGCCCCGACCCGGCCTCGGCAC
GAAGAACGGGCGCCTGCACCAGGTTCCCCACATCGCGGGACTGATCCTGGGCGTCTTCTCGGTGCTCGTGTTCCTGTGGA
GCATTTCGCCGGTGCTGCGCTACTACGTGCACGTGCCGCGGGAGTACGTCGACACCTACTACTTCGACGCCCCCGACACC
AGCCTGTCGTGGGCTCTGGTGGTCGCGCTGCTCGCGGCGGCGCTGGCGAGTCGCAAGCGGATCGCCTGGTGGCTGCTCAC
GATCTACCTGGTTCTGTTCCTGATCACCAACGTGATCGTGAGCATCACGGACAAGAACGTGAATGCGATGGCCGCCGCCG
TGGTCCAGGTGGTGCTGATCGGCATCCTCATCGCGGCCCGCCCGGAGTTCTACACCCGAGTCCGGCGCGGCGCCGGCTGG
AAAGCCCTCGGCGTACTCATCGTCGGGCTCGCCATCGGCACGCTCCTCGGCTGGGGCCTCGTCGAACTGTTCCCCGGCAC
CCTGCCGCAGAATGAACGTTTCCTGTGGGCGCTGAATCGCGTGACCGCGCTCGCCGCCGCCGACAACGAACAATTCACCG
GGCGCCCACACGGATTCGTCAACACCCTCCTCGGCCTGTTCGGTGCGATCGCACTTCTCGCCGCCGTCATCACTCTGTTC
CGTGCCCAACGCTCCCACAACGCGCTCACCGGCAACGACGAATCGGCGTTGCGCGGGCTCCTGCTGCAGTACGGCGCGGA
CGACTCCCTCGGCTACTTCGCCACCCGCCGCGACAAGGCCGTCGTGTTCGCACCCAGCGGGAAGGCGGCCATCACCTACC
GCGTCGAGCTCGGCGTGTGCCTGGCGAGCGGCGACCCGATCGGCGACCCGGAGGCGTGGCCCCACGCGATCGAGGAGTGG
CAGGCACTCGCGTCCCAATACGGCTGGGCCACCGCCGTGATGGGCGCCAGCGAAACCGGCGCCACCGCGTACAACAAGGC
CGGTCTCACCGTGCTCCAGCTGGGCGACGAAGCCATCCTCCGCACCCGCGAATTCAACCTCAGCGGGCGCGACATGCGGC
AGGTCCGCCAGGCCGTCACCCGTGTCCGGCGTCAGGGCGTCACCGTCCGGATCCGCAGACACCGGGACGTCCCGGCGGAG
GAGATGGCCGAGACCATCCGGCTCGCGGACGCGTGGCGCGACACCGAGACCGAGCGCGGTTTCTCCATGGCCCTCGGCCG
GCTCGGTGACCGCCTCGACGGCGACTGCCTGCTGGTGGAGGCGGTCGCCGAGGACGGCGAGATCGACGGCATCCTGTCGT
TGGTGCCGTGGGGGCCGACCGGTGTCTCCCTCGACCTGATGCGACGCAAGCCGACGTCCCCCAACGGGGTGGTGGAGCTG
ATGGTGTCCGAACTGGCCACCACCTCGGATCAATTCGGCATCACCAAGGTGTCACTGAACTTCGCGGTGTTCCGCTCCGT
CTTCGAGGAAGGCTCCCGCATCGGGGCCGGACCCATCCTGCGGATCTGGCGTTCGATCCTCGTCTTCTTCTCCCGCTGGT
GGCAGCTCGAGGCGCTGTACCGGTCGAACGTGAAGTACCAGCCGGAATGGGTGCCGCGGTTCCTCTGCTTCGAGGACAAC
CGGGAACTGCTGCGCGTCGGATTCGCGTCGGCCGTCGCGGAAGGATTCGTGACCCTGCCCCGCTTCGGCCGCTCCGGAAC
CCGGGACGCCCTCGAGCACACCGGCACCCACGCCGCGGTCCCCGCCGCACTCGTCGCCGCCGAAGGACTGCACTCCGACG
GCAGCGCACCCGGCGAAGGCCTCGCACCGACGGCGACCGGCCCGAAGCGTCCCGAGCAGGTCCGCGTCCGGCTCGACAAA
CTGGCCGGCCTCGCCGAACAGGGCATCGATCCCTATCCCGTCGCGTATCCGCCCAGCCACACGGTGACCGAGGCCGTGGA
GTCGCCGGAGGGAACGACGGTCCGCATCGCGGGCCGCCTGCTCCGGATCCGCGACTACGGCGGCGTCGTGTTCGCGGTGG
TCCGGGACTGGTCCGGCGACATCCAGATCCTCGTCGACGAGGCCCGGGTGGGTACCGACCGCATCCGGGCGTTCGCCGCC
GAGTTCGACCTCGGTGACCTCGTCGAGGTCGCCGGCGTCATCGGATACAGCCGCCGCGGCGCGCTGTCGCTGCTCGCGAA
CGAATGGCGCATGACCGGCAAGTGCCTGCACCCCCTGCCGGACAAGTGGAAGGGCCTGTCCGATCCGGAGACCCGCGTCC
GGCAGCGGTACGTCGACCTGGCCATCAACTCCGATGCCCGCCGACTGCTCGAGGCCCGCAGCGCCGTCGTGAAGTCGCTG
CGCGATTCGCTCGGCGGGCGCGGCTTCCTCGAGGTGGAGACACCGATCCTGCAGCAGGTGCACGGCGGCGCCAACGCGGC
CCCGTTCCTCACCCACATCAACGCCTACAACCTGGACCTGTACCTGCGGATCGCGCCGGAGCTGTACCTCAAGCGGCTGT
GCGTCGCCGGCATGGAGAAGGTGTTCGAGATCGGCCGCGTCTTCCGCAACGAGGGCGTCGATTTCAAACACAATCCCGAG
TTCACGATCCTCGAGGCGTACGAGGCGCACAGCGACTACGAGAAGATGATGGTGCTGTGCCGGGAGCTCATCCAGACCGC
GGCGATCGCGGCGTACGGACGCGAGATCATCATGCGGCCCGGCCCCGACGGCGCGCTGGTGGAGGTCGACATCTCCGGCG
AATGGCCCGTCAAGACGATGCACCAGGCGGTCGCCGAGAAGCTCGGCGTCGACGTCTCACCGGAAACGCCGCTAACGGAA
CTGCAACGGCTCTGCGACGAGCACGAGATCCCGTACCAGTCCACCTGGGACGCCGGCGCCGTCGCGCAGGAAATGTACGA
GCACCTGGTGGAGGACTACACCGAGTTCCCCACCTTCTACACCAACTTCCCGACGTCGATGTCACCGCTCACCCGGCCGC
ACCCCACGATTCCCGGGGTCGCCGCGAAGTGGGACCTCGTCGCGTGGGGCGTCGAACTCGGCACCGCGTACAGCGAACTC
ACCGACCCGGTGGATCAGCGGAACCGGCTCACCGAGCAGTCGATGCTCGCGGCCGGCGGTGACGAGGAGGCGATGGAACT
CGACGAGGACTTCCTGCAGGCCCTCGAGCACGCCATGCCCCCGACCGGCGGGCTCGGCATGGGCGTCGACCGGGTGGTCA
TGCTCATCACCGGCGGCAGCATCCGTGAAACCCTCGCGTTCCCCCTGGCCAAGCCGAGGCAATGA

Protein sequence :
MSASTETHHASEAAVPTAPRPRPGLGTKNGRLHQVPHIAGLILGVFSVLVFLWSISPVLRYYVHVPREYVDTYYFDAPDT
SLSWALVVALLAAALASRKRIAWWLLTIYLVLFLITNVIVSITDKNVNAMAAAVVQVVLIGILIAARPEFYTRVRRGAGW
KALGVLIVGLAIGTLLGWGLVELFPGTLPQNERFLWALNRVTALAAADNEQFTGRPHGFVNTLLGLFGAIALLAAVITLF
RAQRSHNALTGNDESALRGLLLQYGADDSLGYFATRRDKAVVFAPSGKAAITYRVELGVCLASGDPIGDPEAWPHAIEEW
QALASQYGWATAVMGASETGATAYNKAGLTVLQLGDEAILRTREFNLSGRDMRQVRQAVTRVRRQGVTVRIRRHRDVPAE
EMAETIRLADAWRDTETERGFSMALGRLGDRLDGDCLLVEAVAEDGEIDGILSLVPWGPTGVSLDLMRRKPTSPNGVVEL
MVSELATTSDQFGITKVSLNFAVFRSVFEEGSRIGAGPILRIWRSILVFFSRWWQLEALYRSNVKYQPEWVPRFLCFEDN
RELLRVGFASAVAEGFVTLPRFGRSGTRDALEHTGTHAAVPAALVAAEGLHSDGSAPGEGLAPTATGPKRPEQVRVRLDK
LAGLAEQGIDPYPVAYPPSHTVTEAVESPEGTTVRIAGRLLRIRDYGGVVFAVVRDWSGDIQILVDEARVGTDRIRAFAA
EFDLGDLVEVAGVIGYSRRGALSLLANEWRMTGKCLHPLPDKWKGLSDPETRVRQRYVDLAINSDARRLLEARSAVVKSL
RDSLGGRGFLEVETPILQQVHGGANAAPFLTHINAYNLDLYLRIAPELYLKRLCVAGMEKVFEIGRVFRNEGVDFKHNPE
FTILEAYEAHSDYEKMMVLCRELIQTAAIAAYGREIIMRPGPDGALVEVDISGEWPVKTMHQAVAEKLGVDVSPETPLTE
LQRLCDEHEIPYQSTWDAGAVAQEMYEHLVEDYTEFPTFYTNFPTSMSPLTRPHPTIPGVAAKWDLVAWGVELGTAYSEL
TDPVDQRNRLTEQSMLAAGGDEEAMELDEDFLQALEHAMPPTGGLGMGVDRVVMLITGGSIRETLAFPLAKPRQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lysX YP_003784331.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 2e-159 42
lysS1 YP_005686365.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 2e-159 42
lysS1 YP_005682181.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 6e-159 42
lysS1 YP_005684273.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 2e-159 42