Gene Information

Name : pyk (CPF_0351)
Accession : YP_694808.1
Strain : Clostridium perfringens ATCC 13124
Genome accession: NC_008261
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 419427 - 420830 bp
Length : 1404 bp
Strand : +
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGAGAAGAACAAAATTAGTATGCACTATCGGACCAGCTAGTGAAAACGAAGAAATCTTAACAAAAATAATCGAAGCAGG
TATGAACGCTTCAAGACACAATTTCTCACATGGTGACCATGAAGAACATAAAGGAAGAATGGTTAAAGTAAGAGAAATAT
CAAAAAAATTAGGTAAAGAAGTAGCTATATTATTAGATACTAAAGGACCTGAAATAAGAACTGGAAAATTCGAGCCAAGC
AAAGTTGAATTAACTGCTGGAACTGAATTTACTATATATGCTGGAGCAGAAGACGTAATAGGAGACACTACTAAGTGTTC
AGTTACATATGCTGGATTAGCTAAAGACGTTAAAGCTGGAGATACTATCTTAATAGACGACGGTTTAGTTGGATTAGAAG
TTGTATCTGTAGAAGGAAACGCTGTTAAATGTGTAGTAAGAAACACTGGATTAGTTGGAACTCACAAAGGAGTTAACGTT
CCTGGAGTATCAATAAAATTACCAGCTATGACTGATAAAGATAGAGCTGACTTAATATTCGGTTGTGAAATGGGCGTTAA
CATGGTTGCTGCTTCATTCATAAGAAAAGCAGAAGACGTTAAAGCTATAAGAGAAGTATTAATTGCTAACGGTGGAGCTG
ATATACAAATATTCTCAAAAATCGAAAACCAAGAAGGTGTTGATAACATAGATGCTATCATCGAAGCTTCAGACGGTATC
ATGGTAGCTAGAGGAGACCTTGGTGTTGAAATACCAATGGAAGACGTTCCTTCAGTTCAAAAAATGATCATCGAAAAATG
TAACAATGCTGGAAAACCAGTTATAACTGCTACTCAAATGTTAGACTCAATGATGAGAAACCCAAGACCAACAAGAGCTG
AGGTTTCAGACGTAACTAACGCTATCTTAGATGGTACTGATGCTATCATGTTAAGTGGTGAGTCAGCTAACGGAAGCTGG
CCAGTAGAAGCTGTTGAAACTATGGTTAAAATAGCTACTAAATCAGAAGAAATGTTAAGCTACGAATTAGCTTCATCAAA
AGCTAAAAAACATATACCAGCTGTACCAGGAGTTATCTCAAGAGCTGCTTGTAATGCTGCACACGAGTTAAAATCAGCTG
CTATAGTTTCATTAACTCAATCAGGAGCTACTGCTAAGAGAATATCTCAATGCAGACCAGATGCACCAATCGTTACTGTA
ACTCCAAATGAAAGAGTTGCTAAGAAAGTTGCTTTATGCTTCGGAGTTTACCCAGTTGTTGCTGAAAATGCTACAATGGA
AAACGCTGTTGAAATAGCTAAAAATGCTGGATTCGTTAAAGCTAATGACACTGCTGTAGTTGTTGCAGGTGTACCAGCTA
ACGAAGGAAACACTAACATAGTAAAAGTTGAAGTTGTAAAATAA

Protein sequence :
MRRTKLVCTIGPASENEEILTKIIEAGMNASRHNFSHGDHEEHKGRMVKVREISKKLGKEVAILLDTKGPEIRTGKFEPS
KVELTAGTEFTIYAGAEDVIGDTTKCSVTYAGLAKDVKAGDTILIDDGLVGLEVVSVEGNAVKCVVRNTGLVGTHKGVNV
PGVSIKLPAMTDKDRADLIFGCEMGVNMVAASFIRKAEDVKAIREVLIANGGADIQIFSKIENQEGVDNIDAIIEASDGI
MVARGDLGVEIPMEDVPSVQKMIIEKCNNAGKPVITATQMLDSMMRNPRPTRAEVSDVTNAILDGTDAIMLSGESANGSW
PVEAVETMVKIATKSEEMLSYELASSKAKKHIPAVPGVISRAACNAAHELKSAAIVSLTQSGATAKRISQCRPDAPIVTV
TPNERVAKKVALCFGVYPVVAENATMENAVEIAKNAGFVKANDTAVVVAGVPANEGNTNIVKVEVVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 4e-100 52
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 4e-100 52
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 9e-101 52
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-99 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_694808.1 pyruvate kinase VFG0479 Protein 2e-100 52