Gene Information

Name : lysS (CPF_2781)
Accession : YP_697153.1
Strain : Clostridium perfringens ATCC 13124
Genome accession: NC_008261
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3043495 - 3045000 bp
Length : 1506 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCAAATGAGGAAATAAATATTCACGAAGCAGAAGAACAATTAAGTGAACAAGAGATGTTAAGAAGACAAAAGCTTGC
TGAATTACAAGAAGCTGGTAAGGACCCATTTGATGTATACAAGGTAGAGAGAACACACTCTTCTGCTGATGTAAAAGATA
ATTTTGAAGAGTTAGAAGGTAAAGAAGTAAAAGTTGCTGGAAGACTTATGTCAAAAAGAGGTCAAGGTAAAGTAGTATTC
GCTGATTTAGCAGATTTACCAGGAAAAATTCAATTATTTATTAAGATTGATAACGTTGGAGAGGAAGCTTTAAAGGAATT
TAAAACTTTCGATTTAGGTGACTGGGTTGCTGCAACTGGAGAAGTATTTAAAACAAAAATGGGTGAAGTTTCAGTAAAAG
TAACTTCATTTGAATTAATCTGTAAATCTTTAAAACCATTACCAGAAAAATGGCATGGTTTAAAAGACCCAGACTTAAGA
TACAGACAAAGAGAAGTTGATATAATAACAAACCCAGAAGTTAAAGATACTTTTATTAAGAGATCACAAATAGTAAAAGC
AATAAGAGAATTCTTAGATAATAGAGGATTCTTAGAGGTTGACACACCAATTCTTTCACCAATAGCTGGTGGAGCTGCTG
CTAGACCTTTCATAACTCACCACAATGCTTTAGATATAGATATGTATTTAAGAATTGCTACTGAGTTATACTTAAAGAGA
TTAATAGTTGCTGGATTTGAAAAAGTTTATGAAATGGGTAAAAACTTCAGAAATGAAGGGGTTTCAGTAAGACATAATCC
AGAATTCACTGCTATAGAGTTATATGAAGCATATGCTGATTACAATGACATGATGGAAATCATGGAAAACATGATTGCTT
ATGTTTGTGAAAAAGTAAATGGATCAACTAAAGTTACTTATGAAGGAACTGAAATAGACTTCACACCACCATGGAGAAGA
ATTACTATGGTTGATGCAGTTAAAGAATTTGCTGGTATAGATTTCAACGAAATTAAGAGTGATGAAGAAGCTCAAGCTAT
AGCTAAAGAGAAAAACTTAGAATTCCCTAAACCATTAGATAAGGTTACTAAAGGTGAAGTTCTAAATATGTTATTTGAAG
AATATGGTGAAGATAAATTAATCCAACCTACATTCTTAATAGATTATCCAGTAGAGATATCACCTCTTACTAAAAAGAAA
AGAGGAAATGAAATGTTTACTGAGAGATTTGAAGGATTTGTATATGGTAGAGAAGTATGTAACGCATACTCAGAGCTTAA
CGATCCAATAGTTCAAAGAGAAAGATTTGAACAACAAGCTAGAGAAAGAGAATACGGAGATGATGAAGCATACATGTTAG
ATGAAGAATTCATGAGTGCTTTAGAAACTGGTATGCCTCCAACAGGTGGATTAGGAATAGGAATAGACAGAATGATAATG
TTCTTAACTGACTCTTCATCAATAAGAGACGTTATATTATTCCCAACAATGAAACCACAAAAATAG

Protein sequence :
MSNEEINIHEAEEQLSEQEMLRRQKLAELQEAGKDPFDVYKVERTHSSADVKDNFEELEGKEVKVAGRLMSKRGQGKVVF
ADLADLPGKIQLFIKIDNVGEEALKEFKTFDLGDWVAATGEVFKTKMGEVSVKVTSFELICKSLKPLPEKWHGLKDPDLR
YRQREVDIITNPEVKDTFIKRSQIVKAIREFLDNRGFLEVDTPILSPIAGGAAARPFITHHNALDIDMYLRIATELYLKR
LIVAGFEKVYEMGKNFRNEGVSVRHNPEFTAIELYEAYADYNDMMEIMENMIAYVCEKVNGSTKVTYEGTEIDFTPPWRR
ITMVDAVKEFAGIDFNEIKSDEEAQAIAKEKNLEFPKPLDKVTKGEVLNMLFEEYGEDKLIQPTFLIDYPVEISPLTKKK
RGNEMFTERFEGFVYGREVCNAYSELNDPIVQRERFEQQAREREYGDDEAYMLDEEFMSALETGMPPTGGLGIGIDRMIM
FLTDSSSIRDVILFPTMKPQK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-98 45
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 2e-79 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_697153.1 lysyl-tRNA synthetase VFG1668 Protein 1e-98 45