Gene Information

Name : pyk (CPF_2404)
Accession : YP_696827.1
Strain : Clostridium perfringens ATCC 13124
Genome accession: NC_008261
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2674816 - 2676240 bp
Length : 1425 bp
Strand : +
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGCAAAAAACAAAAATGATATTTACAATTGGTCCTGCAAGTGATTCAGAAGAAATTCTAAGAGAGTTCATAAGAATTGG
TATGAATGCAGCTAGATTAAACTTCTCACATGGAGACCATGCAAGTCATAAAGAAAAGATAGAACTTATTAAGAGACTTA
GAAAAGAAGAAAAATCAGCTACAGCTATCTTATTAGACATTAAGGGTCCTAAAATAAGAACACATAATTTTAAAAATGGC
GAAGCTGAATTAAAAAATGGTGATGAATTCACATTCTCTTGCGGAGATGAAATATTAGGTGATAACACTAAATGTTCAAT
CTCTTATAAAGAACTATATGAAGACATAAAACCTGGTGGATCAATACTTGTTGATGATGGACTTTTAGAATTTAAAGTAA
AGGAAGTCCGTGGAACTGATATAATATGTGAAGTTATTGAAGGAGGTACTATAAAAGACCATAAAGGTGTAAATGTACCT
AATGTACCAATCAAACTCCCTGCAGTAACTGAAAAGGATAGATCTGACTTAATATTTGGTTGCGAAATGGAAGTAGACTT
TGTAGCAGCTTCTTTCATAAGAAAACCTGAGGATGTACTTGAAGTTAGAGAAATTCTAGACTCTCATGGTGGAAAAGATA
TAAAAATAATATCAAAAATAGAAAGCCAAGAAGGTGTAGATAACATAAAAGAAATAATTAAGGTTACTGATGGTGTAATG
GTTGCTAGAGGTGATATGGGAGTAGAAATTCCTATAGAAAATGTACCTATAATTCAAAAAAATATAATTAAGAAATGTAA
TCAAGCAGGGAAAATAGTTATAACGGCAACTCAAATGTTAGATTCTATGATAAGAAATCCAAGACCTACTAGAGCTGAAG
CTTCTGACGTATGTAATGCCATATTTGATGGTACAGATGCCATAATGCTTAGTGGAGAAAGTGCTTCTGGTAGCTTCCCT
ATAGAGGCAGCAATGACAATGTCTAGAATAGCAAAAAAAGCAGAAGCTAACTTAGACTATAATTATCTTTTAAGAAGATT
AAAGGATCCAAATCCAAATCCAGATGCCTTTGCAGATGCCATAAGTTATTCAGCATCAAAAACAGCTAGCAAATTCCCTA
CAAAAGCTATAGTAGCAGCCACTCAGACTGGTTCAACAGCTAAGATATTATCAAAATATAAACCTAGCTGTCCAATAATA
GCTATTACTCCATATGAAAAAGTTAGAAGAAGCCTAGCTTTAAACTTTGGAATAATTTCTAAGAAATGCGCTTATTTTAA
TAGCACAGATGAGATCATAGAGGAAGCTAGAAAAGTAGCTAAAGAATTCCAAATAGCTGAAACTGGAGATAATATAATGG
TAGCTGCTGGGTTCCCAACATCAATAACTGGTGGAACAAACATGCTAAAAATTGAAAAAATATAA

Protein sequence :
MQKTKMIFTIGPASDSEEILREFIRIGMNAARLNFSHGDHASHKEKIELIKRLRKEEKSATAILLDIKGPKIRTHNFKNG
EAELKNGDEFTFSCGDEILGDNTKCSISYKELYEDIKPGGSILVDDGLLEFKVKEVRGTDIICEVIEGGTIKDHKGVNVP
NVPIKLPAVTEKDRSDLIFGCEMEVDFVAASFIRKPEDVLEVREILDSHGGKDIKIISKIESQEGVDNIKEIIKVTDGVM
VARGDMGVEIPIENVPIIQKNIIKKCNQAGKIVITATQMLDSMIRNPRPTRAEASDVCNAIFDGTDAIMLSGESASGSFP
IEAAMTMSRIAKKAEANLDYNYLLRRLKDPNPNPDAFADAISYSASKTASKFPTKAIVAATQTGSTAKILSKYKPSCPII
AITPYEKVRRSLALNFGIISKKCAYFNSTDEIIEEARKVAKEFQIAETGDNIMVAAGFPTSITGGTNMLKIEKI

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 6e-92 48
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 5e-92 48
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 5e-92 48
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-91 48

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_696827.1 pyruvate kinase VFG0479 Protein 2e-92 48