Gene Information

Name : entE (SFV_0542)
Accession : YP_688104.1
Strain : Shigella flexneri 8401
Genome accession: NC_008258
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 572797 - 574407 bp
Length : 1611 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCACT
GACCGACATTCTGACTCGCCACGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCGGCAGTTGAGTTATCGGGAGC
TGAATCAGGCGGCGGATAACCTCGCCTGTAGTTTGCGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTG
GGTAACGTCGCTGAACTTTACATTACCTTTTTCGCGCTGCTGAAACTGGGCGTCGCGCCGGTGCTGGCGCTGTTCAGCCA
TCAGCGTAGTGAACTGAACGCCTACGCCAGCCAGATTGAACCGGCGTTACTGATTGCCGATCGCCAGCATGCGCTATTTA
GCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCCATTCGCGTGGTGCAACTGCACAACGACAGCGGTGAG
CATAACTTGCAGGATGCGATTAACCATCCGGCAGAGGGTTTTACTGCCACGCCATCACCTGCTGATGAAGTGGCCTATTT
CCAGCTTTCCGGCGGCACCACCGGCACACCGAAACTGATCCCGCGCACTCATAACGACTATTACTACAGCGTGCGTCGTA
GCGCCGAGATTTGTCAGTTCACACAACAGACGCGCTACCTGTGCGCGATCCCGGCGGCTCATAACTACGCTATGAGTTCG
CCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCCACGCTTTGCTTCCCGTT
GATTGCAAAACATCAGGTTAACGTTACCGCGCTGGTGCCGCCGGCAGTCAGCCTGTGGTTGCAGGCGCTGGCTGAAGGCG
AAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACGCTTGCAGCGCGTATT
CCCGCTGAGATTGGCTGCCAGTTGCAGCAGGTGTTTGGCATGGCGGAAGGGCTGGTGAACTACACCCGTCTTGATGATAG
CGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGACGATGAAGTGTGGGTTGCCGATGCCGAAGGAAATC
CACTGCCGCAAGGGGAAGTTGGACGCCTGATGACGCGTGGGCCGTACACCTTCCGTGGCTATTACAAAAGTCCGCAGCAC
AATGCCAGTGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGT
GCAGGGGCGCGAGAAAGATCAGATCAACCGTGGCGGCGAGAAGATCGCTGCCGAAGATATCGAAAACCTGCTGCTGAGCC
ATCCGGCAGTGATCTATGCCGCACTGGTGAGCATGGAAAATGAGCTGATGGGCGAAAAAAGCTGTGCTTATCTGGTGGTA
AAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGT
GGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCG
CATCAGCCTGA

Protein sequence : 
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENLLLSHPAVIYAALVSMENELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 6e-88 43
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-88 43
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 8e-88 43
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-88 43
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-88 43
ybtE CAA21394.1 - Virulence HPI Protein 8e-88 43
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 4e-87 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-87 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-87 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_688104.1 enterobactin synthase subunit E VFG0932 Protein 0.0 99
entE YP_688104.1 enterobactin synthase subunit E VFG0168 Protein 2e-117 47
entE YP_688104.1 enterobactin synthase subunit E VFG1817 Protein 1e-86 41