Gene Information

Name : SFV_3860 (SFV_3860)
Accession : YP_691175.1
Strain : Shigella flexneri 8401
Genome accession: NC_008258
Putative virulence/resistance : Unknown
Product : insertion sequence 2 OrfA protein
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG2963
EC number : -
Position : 3962738 - 3963193 bp
Length : 456 bp
Strand : +
Note : IS2 OrfA; forms an overlapping reading frame with orfB; may form a fusion protein OrfAB due to ribosomal frameshifting; both OrfA and OrfAB bind terminal inverted repeats

DNA sequence :
GTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTCAGAACTGATAGTCTTAATACTAGTTTTTAGACTAGTCAT
TGGAGAACAGATTATTGATGTCTTAGAGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGC
AGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGT
AAGCAATACCAGGAAGGAAGTCTTACTGCTGTGGCCGCCGGAGAACAGGTTGTTCCTGCCTCTGAACTTGCTGCCGCCAT
GAAGCAGATTAAAGAACTCCAGCGCCTGCTCGGCAAGAAAACGATGGAAAATGAACTCCTCAAAGAAGCCGTTGAATATG
GACGGGCAAAAAAGTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAA

Protein sequence :
MITDVWKYRGKSTSELIVLILVFRLVIGEQIIDVLEPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWR
KQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYGRAKKWIAHAPLLPGDGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
tnpG AAD44740.1 TnpG Not tested SHI-2 Protein 1e-51 99
unnamed AAL57520.1 IS2 transposase Not tested LEE Protein 1e-51 99
ECO26_5247 YP_003232129.1 insertion sequence 2 OrfA protein Not tested LEE Protein 2e-51 99
st02 CAC81840.1 ST02 protein Not tested LEE II Protein 1e-51 99
S3188 NP_838471.2 insertion sequence 2 OrfA protein Not tested SHI-1 Protein 2e-51 99
S4060 NP_839229.2 insertion sequence 2 OrfA protein Not tested SHI-2 Protein 2e-51 99
aec50 AAW51733.1 Aec50 Not tested AGI-3 Protein 6e-53 99
S4049 NP_839217.2 insertion sequence 2 OrfA protein Not tested SHI-2 Protein 5e-51 98
SF2984 NP_708758.3 IS2 ORF1 Not tested SHI-1 Protein 3e-52 98
SF3709 NP_709448.3 IS2 ORF1 Not tested SHI-2 Protein 1e-51 97
SF3722 NP_709461.3 IS2 ORF1 Not tested SHI-2 Protein 1e-51 97

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
SFV_3860 YP_691175.1 insertion sequence 2 OrfA protein VFG0648 Protein 8e-53 98
SFV_3860 YP_691175.1 insertion sequence 2 OrfA protein VFG0609 Protein 3e-52 97
SFV_3860 YP_691175.1 insertion sequence 2 OrfA protein VFG0622 Protein 3e-52 97