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Name : SFV_3496 (SFV_3496) Accession : YP_690840.1 Strain : Shigella flexneri 8401 Genome accession: NC_008258 Putative virulence/resistance : Unknown Product : insertion sequence 2 OrfA protein Function : - COG functional category : L : Replication, recombination and repair COG ID : COG2963 EC number : - Position : 3565237 - 3565602 bp Length : 366 bp Strand : + Note : IS2 OrfA; forms an overlapping reading frame with orfB; may form a fusion protein OrfAB due to ribosomal frameshifting; both OrfA and OrfAB bind terminal inverted repeats DNA sequence : ATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGA ACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGTAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATACC AGGAAGGAAGTCTTACTGCTGTGGCCGCCGGAGAACAGGTTGTTCCTGCCTCTGAACTTGCTGCCGCCATGAAGCAGATT AAAGAACTCCAGCGCCTGCTCGGCAAGAAAACGATGAAAAATGAACTCCTCAAAGAAGCCGTTGAATATGGACGGGCAAA AAAGTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAA Protein sequence : MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVVASQLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQI KELQRLLGKKTMKNELLKEAVEYGRAKKWIAHAPLLPGDGE |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| tnpG | AAD44740.1 | TnpG | Not tested | SHI-2 | Protein | 6e-52 | 99 |
| S3188 | NP_838471.2 | insertion sequence 2 OrfA protein | Not tested | SHI-1 | Protein | 8e-52 | 99 |
| unnamed | AAL57520.1 | IS2 transposase | Not tested | LEE | Protein | 6e-52 | 99 |
| S4060 | NP_839229.2 | insertion sequence 2 OrfA protein | Not tested | SHI-2 | Protein | 8e-52 | 99 |
| st02 | CAC81840.1 | ST02 protein | Not tested | LEE II | Protein | 6e-52 | 99 |
| S4049 | NP_839217.2 | insertion sequence 2 OrfA protein | Not tested | SHI-2 | Protein | 2e-52 | 99 |
| ECO26_5247 | YP_003232129.1 | insertion sequence 2 OrfA protein | Not tested | LEE | Protein | 8e-52 | 99 |
| SF3709 | NP_709448.3 | IS2 ORF1 | Not tested | SHI-2 | Protein | 3e-52 | 99 |
| SF3722 | NP_709461.3 | IS2 ORF1 | Not tested | SHI-2 | Protein | 3e-52 | 99 |
| SF2984 | NP_708758.3 | IS2 ORF1 | Not tested | SHI-1 | Protein | 1e-51 | 99 |
| aec50 | AAW51733.1 | Aec50 | Not tested | AGI-3 | Protein | 7e-52 | 99 |
| unnamed | AAL67376.1 | insertion sequence protein | Not tested | PAI II CFT073 | Protein | 3e-37 | 89 |
| insC | YP_002414034.1 | IS2 insertion element repressor InsA; KpLE2 phage-like element | Not tested | Not named | Protein | 1e-36 | 89 |
| insC | YP_854262.1 | IS2 transposase | Not tested | PAI I APEC-O1 | Protein | 1e-29 | 76 |
| c5196 | NP_757044.1 | transposase insC | Not tested | PAI II CFT073 | Protein | 1e-29 | 76 |
| insC | AAZ04431.1 | IS2 conserved hypothetical protein | Not tested | PAI I APEC-O1 | Protein | 9e-30 | 76 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| SFV_3496 | YP_690840.1 | insertion sequence 2 OrfA protein | VFG0648 | Protein | 3e-52 | 99 |
| SFV_3496 | YP_690840.1 | insertion sequence 2 OrfA protein | VFG0622 | Protein | 8e-53 | 99 |
| SFV_3496 | YP_690840.1 | insertion sequence 2 OrfA protein | VFG0609 | Protein | 8e-53 | 99 |
| SFV_3496 | YP_690840.1 | insertion sequence 2 OrfA protein | VFG1733 | Protein | 4e-37 | 89 |
