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Name : SFV_3088 (SFV_3088) Accession : YP_690456.2 Strain : Shigella flexneri 8401 Genome accession: NC_008258 Putative virulence/resistance : Unknown Product : insertion element IS2 transposase InsD Function : - COG functional category : L : Replication, recombination and repair COG ID : COG2801 EC number : - Position : 3152586 - 3153437 bp Length : 852 bp Strand : + Note : IS2 OrfB; forms an overlapping reading frame with orfA; may form a fusion protein OrfAB due to ribosomal frameshifting; in some cases OrfB is itself expressed; both OrfA and OrfAB bind terminal inverted repeats DNA sequence : GTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCGGGTGTCGCGTGCGCA GTTGCACGTCATTCTCAGACGAACCGATGACTGGATGGATGGCCGCCGCAGTCGTCACACTGATGATACGGATGTGCTTC TCCGTATACACCATGTTATCGGAGAGCTGCCCACGTATGGTTATCGTCGGGTATGGGCGCTGCTTCGCAGACAGGCAGAA CTTGATGGTATGCCTGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCTTGAGCGAAAACC TGCTGTACCGCCATCGAAACGGGCACATACAGGCAGAGTAGCCGTGAAAGAAAGCAATCAGCGATGGTGCTCTGACGGGT TCGAGTTCTGCTGTGATAACGGAGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGG GCGGGGACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCGGTGGAACGCCGCTTCGGCAACGA TCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGA TGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATA AAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTA TAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGGGTAA Protein sequence : MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAE LDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHW AGTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTI KRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPRG |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| SF3708 | NP_709447.1 | IS2 transposase TnpB | Not tested | SHI-2 | Protein | 1e-129 | 99 |
| SF3721 | NP_709460.1 | IS2 transposase TnpB | Not tested | SHI-2 | Protein | 1e-129 | 99 |
| S4061 | NP_839230.2 | insertion element IS2 transposase InsD | Not tested | SHI-2 | Protein | 4e-130 | 99 |
| S3189 | NP_838472.2 | insertion element IS2 transposase InsD | Not tested | SHI-1 | Protein | 1e-128 | 99 |
| S4050 | NP_839218.2 | insertion element IS2 transposase InsD | Not tested | SHI-2 | Protein | 1e-129 | 99 |
| tnpF | AAD44739.1 | TnpF | Not tested | SHI-2 | Protein | 3e-112 | 99 |
| SF2985 | NP_708759.1 | insertion element IS2 transposase InsD | Not tested | SHI-1 | Protein | 3e-110 | 98 |
| insD | YP_002414033.1 | insertion element IS2 transposase InsD | Not tested | Not named | Protein | 2e-126 | 97 |
| unnamed | CAC39284.1 | hypothetical protein | Not tested | LPA | Protein | 7e-111 | 97 |
| unnamed | AAL67379.1 | transposase-like protein | Not tested | PAI II CFT073 | Protein | 9e-109 | 96 |
| ECO111_3773 | YP_003236109.1 | putative IS2 transposase InsAB | Not tested | LEE | Protein | 5e-112 | 93 |
| aec49 | AAW51732.1 | Aec49 | Not tested | AGI-3 | Protein | 2e-114 | 85 |
| unnamed | CAC39283.1 | hypothetical protein | Not tested | LPA | Protein | 4e-82 | 63 |
| c5197 | NP_757045.1 | transposase insD | Not tested | PAI II CFT073 | Protein | 6e-62 | 60 |
| insD | YP_854263.1 | IS2 transposase | Not tested | PAI I APEC-O1 | Protein | 9e-66 | 60 |
| insD | AAZ04432.1 | IS2 transposase | Not tested | PAI I APEC-O1 | Protein | 6e-66 | 60 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| SFV_3088 | YP_690456.2 | insertion element IS2 transposase InsD | VFG0621 | Protein | 4e-130 | 99 |
| SFV_3088 | YP_690456.2 | insertion element IS2 transposase InsD | VFG0608 | Protein | 4e-130 | 99 |
| SFV_3088 | YP_690456.2 | insertion element IS2 transposase InsD | VFG0649 | Protein | 1e-110 | 98 |
| SFV_3088 | YP_690456.2 | insertion element IS2 transposase InsD | VFG1732 | Protein | 8e-127 | 97 |
