Gene Information

Name : pykF (SFV_1699)
Accession : YP_689173.1
Strain : Shigella flexneri 8401
Genome accession: NC_008258
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1735377 - 1737005 bp
Length : 1629 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGGTTTCAGCACTTTGGACTGTAGAACTCAACGACTCAAAAACAGACACTCACGTTGGGCTGAGACACAAGCACACATT
CCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCA
GTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGC
ACCATCGGACCGAAAACCGAATCTGAAGAGATGTTAGCTAAAATGCTGGACGCTGGCATGAACGTTATGCGTCTGAACTT
CTCTCATGGTGACTATGCAGAACACGGTCAGCGCATTCAGAATCTGCGCAACGTGATGAGCAAAACTGGTAAAACCGCCG
CTATCCTGCTTGATACCAAAGGTCCGGAAATCCGCACCATGAAACTGGAAGGCGGTAACGATGTTTCTCTGAAAGCTGGT
CAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTAC
TGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAG
TTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCA
GCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAAAAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCG
TAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCG
AAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGT
GTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTAT
CACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACGCCA
TCCTCGACGGTACTGACGCAGTGATGCTGTCTGGTGAATCCGCAAAAGGTAAATACCCGCTGGAAGCGGTTTCTATCATG
GCGACCATCTGCGAACGTACCGACCGCGTGATGAACAGCCGTCTCGAGTTCAACAATGACAACCGTAAACTGCGCATCAC
CGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACCCAGGGCGGTA
AATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGACCACCAACGAAAAAACGGCTCATCAG
TTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTGGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGA
ACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACGTCGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTA
CTAACACCGCATCTGTTCACGTCCTGTAA

Protein sequence :
MVSALWTVELNDSKTDTHVGLRHKHTFLCTLFRCHLSLERGTTTFVIPDSLSGSAPRKQVSPILLNLKTKTVMKKTKIVC
TIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAG
QTFTFTTDKSVIGNSEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGVSIALP
ALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHIISKIENQEGLNNFDEILEASDGIMVARGDLG
VEIPVEEVIFAQKMMIEKCIRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIM
ATICERTDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFPDATILALTTNEKTAHQ
LVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVVMVSGALVPSGTTNTASVHVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 0.0 96
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 0.0 96
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 0.0 96
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 0.0 96

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykF YP_689173.1 pyruvate kinase VFG0479 Protein 0.0 96