Gene Information

Name : lysS (CHU_0741)
Accession : YP_677368.1
Strain : Cytophaga hutchinsonii ATCC 33406
Genome accession: NC_008255
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 835405 - 837132 bp
Length : 1728 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGCAATTGAGTGAACAAGAACTGGTACGCCGCCAGGAACGTGAAGAGTTATTAAAAATGGGTATTGAACCATACCCTGC
AGAACTTTTCCCTGTAAATGTTTCAACAAAAGATATTCATCAGAATTACGAAAAGCGAAAGACAGATTATAAAAATATTT
CTATCGCTGGCCGTGTGATGAGCCGCCGTATTATGGGTAATGCCTCTTTCGTTGAGATTCAGGATGCAAGCGGTAGAATC
CAGGCATACTTCAGAAGAGATGATCTTTGTCCGGATGAAGACAAAACACAATACAACACCGTATTTAAAAAGCTTGTCGG
TATCGGTGATATTATTGGTATTTACGGATATGTGTTTACAACACAAACCGGAGAGATCACGATTCATGTTACTTCATTTA
AAATTTTAACAAAAGCACTTCGTCCGTTGCCCATGCCAAAAGAGGCGGTAGATGAGAACGGAGAGAAAAAAATATTTGAT
GCATTCACCGATCCTGAACAGCGTTACCGCATGCGTTATGTGGATCTGATTGTGAACCCGCATGTGCGCGATGCTTTTAT
CAAACGTACACGTCTGGTAAATACCATGCGTGAGTACCTGAATAACAAAGGATATTTAGAAGTGGAAACGCCGATCTTGC
AGCCCTTGTATGGCGGTGCTGCTGCGCGTCCGTTCAAGACACACCACAATACATTAGATATGCCTTTGTATTTGCGTATC
GCAAATGAGTTATATCTGAAACGGTTGATCGTTGGCGGATTTGATGGAGTATATGAATTCTCTAAAGATTTCCGTAACGA
AGGTATGTCCCGTTTCCACAACCCGGAATTCACTCAGGTAGAATTGTATGTTGCATACAAGGATTACTACTGGATGATGG
ATCTGGTTGAAGAGATGGTTGAAAAAGTTGCTGTTGCCTTACACGGTACTACAGAGGTACAGGTTGGTCCGCATACGATC
AGCTTCCAGCGCCCCTGGAAACGTTTCACTATGTTTGAAGCAATCGAACATTATACAGGTATTAACATTGGTGAAATGGA
TGAAGCACAATTACGTGCAACATGTAAATCATTAAATGTGGATGTAGATGATTCAATGGGTAAAGGTAAACTCATTGATG
AGATCTTTGGCGCAAAATGCGAACCATTATTGATCCAGCCTACATTCATTACAGACTATCCGGTTGAAATGTCGCCGTTA
GCGAAGAAACACAGGGACAAGCCAGGTTTGGTTGAACGTTTTGAAGCCATCTGTAATGGTAAAGAAATCTGCAATTCATT
CTCTGAACTGAATGACCCGATTGATCAGCGTCAGCGTTTTGAAGAACAGCTGGAGCTTGGCAAAAGAGGTGACAGCGAGA
GTATGGTACTGGATGAAGATTTTCTTCGTGCACTTGAATACGGAATGCCGCCTACAGCAGGCCTTGGTATTGGGATTGAC
CGGTTAGCGATGGTTATGACTAATTCAGCTTCGATACAGGATGTATTATTCTTCCCGCAGATGCGTCCGGAAGTTGGTAC
ACAGGGAGCATCTTCCAATGGTTTCCAGGGCATAGAGATCCGCGGGGAACTTGTTCCGTTGATTGAGAAATTAGGTATCT
CTACGGTAGAACAATTAAAAGCAGCAAACCCGAATAAATTATTTAATGATTTGTGCGGCTTACGTAAAAAAATGAAGCTT
GAAATCGCAAACCCTACGGCTGATGAGGTGAAGGGATGGCAGGCGTGA

Protein sequence :
MQLSEQELVRRQEREELLKMGIEPYPAELFPVNVSTKDIHQNYEKRKTDYKNISIAGRVMSRRIMGNASFVEIQDASGRI
QAYFRRDDLCPDEDKTQYNTVFKKLVGIGDIIGIYGYVFTTQTGEITIHVTSFKILTKALRPLPMPKEAVDENGEKKIFD
AFTDPEQRYRMRYVDLIVNPHVRDAFIKRTRLVNTMREYLNNKGYLEVETPILQPLYGGAAARPFKTHHNTLDMPLYLRI
ANELYLKRLIVGGFDGVYEFSKDFRNEGMSRFHNPEFTQVELYVAYKDYYWMMDLVEEMVEKVAVALHGTTEVQVGPHTI
SFQRPWKRFTMFEAIEHYTGINIGEMDEAQLRATCKSLNVDVDDSMGKGKLIDEIFGAKCEPLLIQPTFITDYPVEMSPL
AKKHRDKPGLVERFEAICNGKEICNSFSELNDPIDQRQRFEEQLELGKRGDSESMVLDEDFLRALEYGMPPTAGLGIGID
RLAMVMTNSASIQDVLFFPQMRPEVGTQGASSNGFQGIEIRGELVPLIEKLGISTVEQLKAANPNKLFNDLCGLRKKMKL
EIANPTADEVKGWQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 5e-90 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_677368.1 lysyl-tRNA synthetase VFG1668 Protein 3e-90 47