Gene Information

Name : entE (ECP_0626)
Accession : YP_668553.1
Strain : Escherichia coli 536
Genome accession: NC_008253
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 662012 - 663622 bp
Length : 1611 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCACT
GACCGACATTCTGACTCGCCACGCTACGAGTGACAGCATCGCGGTTATCGACGGCGAGCGGCAGTTGAGTTACCGGGAGC
TGAATCAGGCGGCGGATAACCTCGCCTGTAGTTTGCGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTG
GGTAACGTCGCTGAACTTTACATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGCTGTTCAGCCA
TCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCGGCGTTACTGATTGCCGATCGCCAACATGCGCTATTTA
GCGGGGATGATTTCCTCAACACATTTGTTGCAGAGCATTCTTCCATTCGCGTGGTGCAGCTGCTCAACGACAGCGGCGAG
CATAACTTGCAGGATGCGATTAACCATCCGGCTGAGGATTTTACTGCCACGCCGTCACCTGCCGATGAAGTGGCCTATTT
TCAGCTTTCCGGCGGCACCACCGGCACACCGAAACTCATCCCACGCACTCACAATGACTATTACTACAGCGTGCGTCGTA
GCGTCGAGATTTGTCAGTTCACACAACAGACGCGCTACCTGTGCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCA
CCGGGATCGTTGGGGGTATTTCTCGCTGGCGGCACTGTCGTTCTGGCTGCCGACCCCAGCGCCACGCTTTGCTTCCCATT
GATTGAAAAACATCAGGTGAACGCCACCGCGCTGGTGCCGCCAGCAGTCAGCCTGTGGTTGCAGACACTGGCTGAAGGCG
AAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTGCAGGTCGGCGGCGCACGTCTTTCTGCCACGCTTGCGGCGCGTATT
CCCGCTGAGATTGGCTGCCAGTTGCAGCAGGTGTTTGGCATGGCGGAAGGGCTGGTGAACTACACCCGACTTGATGATAG
CGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGCCCGGACGATGAAGTATGGGTTGCCGATGCCGAAGGAAATC
CACTGCCGCAACGGGAAGTTGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGTGGCTATTACAAAAGCCCGCAGCAC
AATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACTGT
GCAGGGGCGCGAGAAAGATCAGATCAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCC
ATCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGTGCTTATCTGGTGGTA
AAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGGATTGCCGAATTTAAATTACCGGATCGCGT
GGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCA
CATCAGCCTGA

Protein sequence : 
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHATSDSIAVIDGERQLSYRELNQAADNLACSLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNATALVPPAVSLWLQTLAEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQREVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRTSA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-87 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-86 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 3e-87 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-87 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-87 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-87 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-87 42
ybtE CAA21394.1 - Virulence HPI Protein 2e-87 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 8e-87 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_668553.1 enterobactin synthase subunit E VFG0932 Protein 0.0 99
entE YP_668553.1 enterobactin synthase subunit E VFG0168 Protein 7e-116 46