Gene Information

Name : Mmcs_3471 (Mmcs_3471)
Accession : YP_640634.1
Strain : Mycobacterium sp. MCS
Genome accession: NC_008146
Putative virulence/resistance : Virulence
Product : AMP-dependent synthetase and ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 3692991 - 3694640 bp
Length : 1650 bp
Strand : +
Note : PFAM: AMP-dependent synthetase and ligase; KEGG: mbo:Mb2405 bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-arcp synthetase

DNA sequence : 
ATGAGCACCGGATTCCAGCATGTGCACAGCGACCTGACCGATGGCTTCGTCCCATTCCCCGAAGACCGCGCCGAGCAGTA
CCGGCGGGCCGGGTACTGGACCGGGCGGCCCCTCGAGTCCCTTCTCCTTGACGCGGCACACCGACGGCCCGATCACCCGG
CCGTCGTCGACGTCGACGGATCCCTCACGTTCGCGGAGCTGACCGCGCGCGCCGACACCGTCGCCGCGGCGCTGGCCGGA
CTCGGGATCCGTCCTGGTGACCGGGTGCTGCTGCAACTACCCAACTCCGTGCGTTTCGCCGTCGCGTTCTTCGGTCTGCT
GCGGGCGGGCGCGGTGCCGGTGATGTGCCTGCCCGGCCACCGCACCGCCGAGCTGGGACACTTCGCCGACGTCAGCGGCG
CGGTGGCGCTGATCGTGCCCGACGAGGTCGGCGGCTTCGACTACCGCGAGATGGCCGCCCAGTTGGTGGCGGACCGGCCC
ACGCTGCGGCACGTGCTCGTCGACGGTGACCCGGGACCGTTCCTATCGTGGGCGGCACTGATCGACAGCGGCGGCGTGGC
ACCGGAGATCGGACCCGTCGACACCTCGCTGCCTGCCCTGCTGCTGGTCTCCGGCGGAACGACCGGACTGCCCAAGCTGA
TCCCCCGCACCCACGACGACTACGTCTACACCGCGGTGTCGAGCGCGCAGGCGTGCCACTTCACCCCCGACGACGTCTAC
CTGGTGGCGTTGCCGGCCGGCCACAACTTCCCGTTGGCGTGCCCCGGCATGCTGGGGGCAATGACCGTGGGGGCGACGAC
GGTGTTCACCGCCGATCCCAGCCCGGAGGAGGCGTTCGCGCTCATCGACAAACACCAGGTGACCGTCACCGGGCTGGTCA
ACGCGCTGGGCAAGCTGTGGGCGCAGGCGTGCGACTGGGAACCGGTGCTGCCGACGTCGCTGCGCCTGGTGCAGGTGGGC
GGTTCGCGGATGAGCCCGGAGGAGGCGCGGTTCATCCTCGATCGCCTGACCCCCGGGCTGTCCCAGATCTTCGGAATGGC
CGAGGGCATGCTGAATTTCACGCGGCCGGGAGATCCGCTCGACGTCGTGGTGCACACCCAGGGCAGGCCGGTCTCCCCGC
ACGACGAGATGCGGGTGGTCGACGAGTCCGGTGTCGAGGTCGCACCGGGTGAAGAGGGCGAACTGCTGGTGCGCGGTCCC
AACACACTCAACGGGTACTACCGGGCCGACGAGGCCAACGCCCGGTGCTTCAGCCCGGACGGCTTCTACCGCACCGGGGA
CCGGGTGCGGATCTTCGCCGACGGCCCGCTGGCCGGCAACGTCGAGGTGACCGGCCGGATCAAGGACGTCATCCACCGCG
GCGGTGAGACGGTTTCGGCGACCGACCTCGAGGACCATCTGCTGACCCACCCCGCGATCTATGCGGCGGCCGCCGTCGCG
CTACCCGACGACTATCTCGGTGAGAAGATCTGCGCCGCAGTGGTGTTCCGCGGCAAGCAGCTCACGCTCGCCGAACTCAA
CGCATTCCTCGACGAACGCGGGGCGTCCACTCACGCCCGGCCCGACGTGCTGGCTGCGATGCCGTCGCTGCCGCTGACCG
CGGTCGGCAAGGTCGACAAGAAGAAGCTCGTCGCCCAGCTGACGGGGTGA

Protein sequence : 
MSTGFQHVHSDLTDGFVPFPEDRAEQYRRAGYWTGRPLESLLLDAAHRRPDHPAVVDVDGSLTFAELTARADTVAAALAG
LGIRPGDRVLLQLPNSVRFAVAFFGLLRAGAVPVMCLPGHRTAELGHFADVSGAVALIVPDEVGGFDYREMAAQLVADRP
TLRHVLVDGDPGPFLSWAALIDSGGVAPEIGPVDTSLPALLLVSGGTTGLPKLIPRTHDDYVYTAVSSAQACHFTPDDVY
LVALPAGHNFPLACPGMLGAMTVGATTVFTADPSPEEAFALIDKHQVTVTGLVNALGKLWAQACDWEPVLPTSLRLVQVG
GSRMSPEEARFILDRLTPGLSQIFGMAEGMLNFTRPGDPLDVVVHTQGRPVSPHDEMRVVDESGVEVAPGEEGELLVRGP
NTLNGYYRADEANARCFSPDGFYRTGDRVRIFADGPLAGNVEVTGRIKDVIHRGGETVSATDLEDHLLTHPAIYAAAAVA
LPDDYLGEKICAAVVFRGKQLTLAELNAFLDERGASTHARPDVLAAMPSLPLTAVGKVDKKKLVAQLTG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 1e-91 44
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-91 44
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-91 44
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-91 44
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-91 44
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-91 44
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 1e-91 44
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-91 44
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-91 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Mmcs_3471 YP_640634.1 AMP-dependent synthetase and ligase VFG1817 Protein 3e-172 67
Mmcs_3471 YP_640634.1 AMP-dependent synthetase and ligase VFG0168 Protein 1e-85 42
Mmcs_3471 YP_640634.1 AMP-dependent synthetase and ligase VFG0932 Protein 2e-80 41