Gene Information

Name : cadA (PSEEN5232)
Accession : YP_610640.1
Strain : Pseudomonas entomophila L48
Genome accession: NC_008027
Putative virulence/resistance : Resistance
Product : cadmium translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : 3.6.3.3
Position : 5548732 - 5550978 bp
Length : 2247 bp
Strand : -
Note : Evidence 2a : Function of homologous gene experimentally demonstrated in an other organism; PubMedId : 11282588; Product type e : enzyme

DNA sequence :
ATGAACCAGCCAGTCAGCCCCGAACACAAGCATGACCACGACCATGGTGAAAAGGGTCACAGCCACGACACCCATGCCCA
CAGCTGCTGCGGCTCCCATGCCGCGCCGGCCCTGGTGCAACTGACCGAGAAGGCCAGCGCCCAGGCCCGGCTCAGCCGTT
TCCGCATCCAGGCCATGGACTGCCCGACCGAGCAGACGCTGATCCAGGACAAGCTGGGCAAGCTGGCCGGTATCGAGCAA
CTGGAGTTCAACCTGATCAACCGCGTGCTGGGCGTGCGCCACACCCACGACGGCACCGGCCAGATCGAGCAGGCCATCGA
CAGCCTGGGCATGAAGGCCGAGCCGCTGGCGGACGAGGACGACGGCAGCGTCGAAGTGCCCCAGGCCAGCAAGACCCGCT
GGTGGCCGCTGGCATTGTCCGGTGTAGCCGCGATCGCCGCCGAGCTCGTGCACTTCTCCGGCAAGGCGCCGGAATGGGTA
GTGGCGGCGCTGGCCCTGGCGGCCATCCTCGGCTGCGGGCTGGGCACCTACAAGAAGGGCTGGATCGCCCTGAAGAACCG
CAACCTCAACATCAACGCCCTGATGAGCATCGCCGTGACCGGCGCCGTGCTGATCGGCCAGTGGCCGGAAGCGGCCATGG
TGATGGTGCTGTTCACCGTGGCCGAACTGATCGAAGCCCGCTCGCTGGACCGCGCGCGCAACGCGATCGGCGGCCTGATG
CAGCTGGCGCCGGATATGGCGACCGTGCGGGAGGCCGATGGGCAATGGCGTGAAGTCGACGTGCGCGAGGTGGCTTTGGG
CGCCCTTGTGCGGGTGCGACCGGGTGAGCGCATCGGCCTGGACGGCGAGGTTGTTAGTGGGCAGTCCAGTGTCGATCAGG
CACCGATCACCGGCGAAAGCCTGCCGGTAGAGAAAACCGTGGGCGACAAACTGTTCGCCGGCACCATCAACCAGGCAGGC
TCGCTGGAGTACCGCGTCAGCGCAGGTGCCGGCCAGTCGACCCTGGCGCGCATCATCAAGGCGGTCGAGGAAGCCCAGGG
CGCCCGTGCGCCGACCCAGCGCTTCGTCGACCAGTTCTCGCGCATCTACACCCCGGTGGTGTTCGCCGTGGCCCTGGCCG
TGGCGATCATCCCACCGCTGTTCATGGCCGGCGCCTGGTTCGACTGGATCTACCGCGCCCTGGTGCTGTTGGTGGTGGCC
TGCCCGTGCGCCTTGGTGATCTCCACCCCGGTGACCATCGTCAGCGGCCTGGCCGCCGCCGCGCGCAAGGGCATCCTGAT
CAAGGGTGGTGTGTACCTGGAAGGCGGGCGCAAGCTGGACTTCCTGGCCCTGGACAAGACCGGCACCATCACCCACGGCA
AGCCGGTACAGACCGACAGCAAGGTGCTCGACCCGTTGTTCGAAGGCCGTGCCCAGGCGCTGGCCGCAAGCCTTGCCGCC
CGCTCCGACCACCCGGTGTCCGGCGCCATCGCCCTGTTCGCCAAGGCGCAAAGCCTGCCATTGAGTGAAGTCACCGAGTT
CGCCGCCCTGGCCGGGCGCGGCGTGCGTGGCGACATCGACGGGGAAACCTACCACCTGGGCAACCATCGCTTGGTCGAGG
AGCTGGGCCTGTGCTCGCCGCAGCTCGAAGCCGAGCTGGACCAGCTGGAACGTCAGGGCAAGACCGTGGTGCTGCTGCTC
GACCGCAGTGGCCCGCTGGCGCTGTTCGCCGTGGCCGACACGGTCAAGGACAGCAGCCGCCAGGCCATCGCCGAACTGCA
TGAGCTCGGTATCAAGACGGTCATGCTCACCGGCGACAATCCCCACACCGCCCAAGCCATTGCTGCCCAGGTGGGGATCG
ACCGGGCCGAGGGCAACCTGCTGCCGGCCGATAAGCTCGCCACCATCGAGCGCCTGTACGCCGAGGGCCACCGGGTCGGT
ATGGTCGGTGACGGTATCAACGACGCACCGGCCCTGGCCCGTGCCGAGATCGGTTTCGCCATGGCCGCCGCCGGTACCGA
CACGGCCATCGAGACCGCCGACGTGGCGCTGATGGACGATGACTTGCGCAAAATCCCGGCGTTCGTCAGGCTGTCGCGCC
AAAGCGCGGCGATCCTCACCCAGAACATCGTGTTGGCCCTGGGGATCAAGGCGATCTTCCTGGCGATCACCTTTGCCGGC
ATGGCCACCATGTGGATGGCGGTGTTCGCCGACATGGGCGTGAGCTTGCTGGTGGTGTTCAACGGCTTGCGCCTGCTACG
CAAATAG

Protein sequence :
MNQPVSPEHKHDHDHGEKGHSHDTHAHSCCGSHAAPALVQLTEKASAQARLSRFRIQAMDCPTEQTLIQDKLGKLAGIEQ
LEFNLINRVLGVRHTHDGTGQIEQAIDSLGMKAEPLADEDDGSVEVPQASKTRWWPLALSGVAAIAAELVHFSGKAPEWV
VAALALAAILGCGLGTYKKGWIALKNRNLNINALMSIAVTGAVLIGQWPEAAMVMVLFTVAELIEARSLDRARNAIGGLM
QLAPDMATVREADGQWREVDVREVALGALVRVRPGERIGLDGEVVSGQSSVDQAPITGESLPVEKTVGDKLFAGTINQAG
SLEYRVSAGAGQSTLARIIKAVEEAQGARAPTQRFVDQFSRIYTPVVFAVALAVAIIPPLFMAGAWFDWIYRALVLLVVA
CPCALVISTPVTIVSGLAAAARKGILIKGGVYLEGGRKLDFLALDKTGTITHGKPVQTDSKVLDPLFEGRAQALAASLAA
RSDHPVSGAIALFAKAQSLPLSEVTEFAALAGRGVRGDIDGETYHLGNHRLVEELGLCSPQLEAELDQLERQGKTVVLLL
DRSGPLALFAVADTVKDSSRQAIAELHELGIKTVMLTGDNPHTAQAIAAQVGIDRAEGNLLPADKLATIERLYAEGHRVG
MVGDGINDAPALARAEIGFAMAAAGTDTAIETADVALMDDDLRKIPAFVRLSRQSAAILTQNIVLALGIKAIFLAITFAG
MATMWMAVFADMGVSLLVVFNGLRLLRK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 0.0 66
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 3e-107 47
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 4e-112 43
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 3e-112 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA YP_610640.1 cadmium translocating P-type ATPase BAC0298 Protein 0.0 69
cadA YP_610640.1 cadmium translocating P-type ATPase BAC0455 Protein 9e-79 42
cadA YP_610640.1 cadmium translocating P-type ATPase BAC0092 Protein 3e-72 41
cadA YP_610640.1 cadmium translocating P-type ATPase BAC0124 Protein 6e-65 41