Gene Information

Name : fliI (PSEEN3813)
Accession : YP_609316.1
Strain : Pseudomonas entomophila L48
Genome accession: NC_008027
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 4090751 - 4092106 bp
Length : 1356 bp
Strand : -
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGCGCCTTGATCGCACCAGCTTCGGCAAGCGCCTCGAAGGTTACGCCGATGCCATCCGCTTGCCGGACCAGCCGGTGGT
GGAAGGCCGCCTGCTGCGCATGGTCGGGTTGACCCTGGAGGCCGAGGGCCTGCGCGCCGCCGTGGGCAGCCGCTGCCTGG
TGATCAACGACGACAGCTATCACCCGGTGCAGGTCGAAGCCGAAGTGATGGGCTTCTCCGGTGACAAGGTGTTCCTCATG
CCGGTCGGCAGCATCGCCGGCCTGGCCCCGGGAGCCCGGGTGGTGCCGCTGGATGACAGTGGCCGCCTGCCCATGGGCAT
GAGCATGCTCGGGCGGGTGCTCGACGGTGCCGGCCGGGCCCTGGATGGCAAGGGTGGGATGAAGGCCGAGGACTGGGTGC
CGATGGATGGCCCGGTCATCAACCCGCTCAACCGCGACCCCATTAGCCAGCCGCTGGATGTCGGCATTCGCAGCATCAAT
GGCTTGCTGACGGTCGGGCGCGGCCAGCGTCTTGGCCTGTTCGCCGGCACCGGTGTCGGTAAGTCGGTGTTGCTGGGCAT
GATGACCTGCTTCACTGAAGCCGAGATCATCGTCGTCGGGCTGATCGGTGAGCGTGGTCGCGAAGTGAAGGAATTCATCG
AGCACATCCTCGGTGAGGAAGGGCTCAAGCGTTCGGTCGTGGTCGCGTCGCCTGCGGACGACGCCCCGTTGATGCGGCTG
CGCGCGGCGATGTATTGCACGCGAATTGCCGAGTATTTCCGCGACAAGGGCAAGAACGTCCTGTTGCTGATGGACTCCCT
GACCCGTTTCGCCCAGGCCCAGCGCGAAATCGCCCTGGCCATCGGCGAGCCACCCGCCACCCGTGGCTATCCGCCTTCGG
TGTTCGCCAAGCTGCCCAAGCTGGTGGAGCGGGCGGGTAACGGCGAGCCGGGTGGCGGGTCGATCACGGCGTTCTATACC
GTGCTATCCGAAGGCGACGACCAGCAGGACCCGATCGCCGACTCGGCGCGGGGTGTGCTCGACGGCCACTTCGTGCTGTC
GCGGCGCCTGGCCGAGGAAGGGCACTACCCGGCCATCGATATCGAAGCGTCCATCAGCCGGGTCATGCCCCAGGTGGTCG
ACCCCGACCACCTGCGCCAGGCGCAGAAGTTCAAGCAGCTGTGGTCGCGCCTGTCGCAGAGCCGCGACCTGATCAGCGTC
GGCGCCTATGTGGCCGGTGGCGACGTGGAGACCGACCTGGCCATCGCCCTGCAGCCCAAGCTGGTGGACTTCCTGCGCCA
GGGTCTGGACGAAAATGTCGGCATGGCCCAGAGCCGCGAAGAGCTGGGCGCAATCTTCGTGCCGCCGGCGGGGTGA

Protein sequence :
MRLDRTSFGKRLEGYADAIRLPDQPVVEGRLLRMVGLTLEAEGLRAAVGSRCLVINDDSYHPVQVEAEVMGFSGDKVFLM
PVGSIAGLAPGARVVPLDDSGRLPMGMSMLGRVLDGAGRALDGKGGMKAEDWVPMDGPVINPLNRDPISQPLDVGIRSIN
GLLTVGRGQRLGLFAGTGVGKSVLLGMMTCFTEAEIIVVGLIGERGREVKEFIEHILGEEGLKRSVVVASPADDAPLMRL
RAAMYCTRIAEYFRDKGKNVLLLMDSLTRFAQAQREIALAIGEPPATRGYPPSVFAKLPKLVERAGNGEPGGGSITAFYT
VLSEGDDQQDPIADSARGVLDGHFVLSRRLAEEGHYPAIDIEASISRVMPQVVDPDHLRQAQKFKQLWSRLSQSRDLISV
GAYVAGGDVETDLAIALQPKLVDFLRQGLDENVGMAQSREELGAIFVPPAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-77 51
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-76 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-76 51
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-76 50
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-76 50
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-83 50
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-87 49
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-87 49
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-86 48
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-86 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 9e-89 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 9e-89 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 5e-89 48
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-88 48
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-88 48
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-81 47
escN YP_003236088.1 translocator EscN Virulence LEE Protein 3e-74 47
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-73 47
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-73 47
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-73 47
escN NP_290268.1 hypothetical protein Virulence LEE Protein 4e-74 47
escN AAC31513.1 L0034 Virulence LEE Protein 3e-74 47
ECs4568 NP_312595.1 EscN Virulence LEE Protein 4e-74 47
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 3e-74 47
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-72 47
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 5e-75 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-73 46
escN AAC38383.1 EscN Virulence LEE Protein 4e-74 46
escN AAK26715.1 EscN Virulence LEE Protein 3e-73 46
escN AAL57542.1 EscN Virulence LEE Protein 3e-73 46
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-68 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 9e-69 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 4e-69 45
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-69 45
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 7e-70 45
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 6e-70 45
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 2e-65 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 7e-69 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-70 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 1e-70 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-69 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-69 44
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-65 43
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-65 43
invC AAS66861.1 InvC Not tested SSR-2 Protein 7e-69 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-67 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_609316.1 flagellum-specific ATP synthase VFG1254 Protein 0.0 87
fliI YP_609316.1 flagellum-specific ATP synthase VFG2499 Protein 2e-123 63
fliI YP_609316.1 flagellum-specific ATP synthase VFG2331 Protein 5e-120 62
fliI YP_609316.1 flagellum-specific ATP synthase VFG0515 Protein 1e-76 50
fliI YP_609316.1 flagellum-specific ATP synthase VFG2144 Protein 3e-84 47
fliI YP_609316.1 flagellum-specific ATP synthase VFG0048 Protein 2e-85 47
fliI YP_609316.1 flagellum-specific ATP synthase VFG0192 Protein 7e-85 47
fliI YP_609316.1 flagellum-specific ATP synthase VFG0812 Protein 2e-74 47
fliI YP_609316.1 flagellum-specific ATP synthase VFG0390 Protein 2e-80 46
fliI YP_609316.1 flagellum-specific ATP synthase VFG0730 Protein 3e-74 46
fliI YP_609316.1 flagellum-specific ATP synthase VFG1008 Protein 5e-61 41
fliI YP_609316.1 flagellum-specific ATP synthase VFG1778 Protein 2e-66 41