Gene Information

Name : fliI (Sden_1327)
Accession : YP_562336.1
Strain : Shewanella denitrificans OS217
Genome accession: NC_007954
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 1548757 - 1550097 bp
Length : 1341 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGCTCAGCCGTCAACATCAACTCCTGAATAAGCTTAAATCTCATGCCGGACGAGTCAGTCCATTGGTCCCTGTTGCCAG
TGGACAGTTAGTGCGGGTGGTGGGGCTGACATTAGAGGCTAGCGGTTGCCGGGCGCCCGTGGGTAGTCTTTGTTCTATCG
AGACCATGGCGGGAGAACTGATCGCTGAAGTGGTGGGCTTTGACGACAAGTTACTTTACCTCATGCCTATTGAGGAACTC
AGAGGCGTGCTGCCAGGTGCCAGAGTGCAACCCCTTGGGGAACAATCAGGCCTTAATGTGGGCTTATCCTTATTAGGCCG
AGTGTTAGATGGCAATGGCTTGCCCCTCGACGGCTTGGGTGCACTCAATACCGATCAACATGCTTCTCGTCATGGTCCTA
GCATTAACCCGCTTTCACGTCGTGCTATCACTGAGCCACTTGATGTGGGAGTGAGAGCCATTAATGCCATGCTAACCGTA
GGTAAAGGTCAGCGAATGGGCTTGTTTGCAGGCTCGGGCGTCGGTAAAAGCGTGCTCTTAGGCATGATGACCCGCGGTAC
TACAGCCGATATTATTGTGGTAGGGCTAGTGGGGGAGCGTGGTCGAGAAGTCAAAGAGTTTATTGAAGAGATTTTAGGCG
AACATGGCCGTGCCCGTTCAGTGGTCGTTGCCGCGCCTGCGGATACATCTCCCTTGATGCGCCTTCGTGCGTGTGAAACG
TCAACGCGTATTGCCGAGTACTTTCGTGATCTAGGTTATAACGTGCTGTTATTGATGGATAGCCTAACTCGTTATGCTCA
GGCTCAGCGCGAAGTTGCCTTAGCCGTCGGTGAACCGCCCGCCACCAAGGGCTATCCCCCCTCAGTGTTCGCTAAGCTGC
CTCGTTTGGTGGAGCGCGCCGGAAATGGTGGCCCGGGTCAAGGTTCAATCACCGCATTTTATACCGTGTTAACTGAGGGC
GATGATCAGCAAGATCCCATTGCCGATGCTTCTAGAGCCATTTTGGATGGCCATATTGTGCTTTCAAGGCGCCTTGCCGA
CTCAGGCCACTACCCTGCCATTGATATCGAGTCTTCCATCAGTCGCGTCGCGCCAATGGTAATCAGTAATGAGCACCTTG
AAGCGATGCGAAGGGTGAAGCAAATGTATTCCATGTACCAACAAAATCGAGACTTAATTTCCATCGGTGCTTATGCCCAA
GGTAGCGATCCGCGCATCGATAATGCCATTCGATTGCAGCCTGCGATGAATGCATTTTTGCGTCAAGGGTTTAAAGAAGC
CATTAGTTTCGATGACAGCCAATTGATGATGAGCCAAATCGCCGCCCAGTGTCAGGTTTAG

Protein sequence :
MLSRQHQLLNKLKSHAGRVSPLVPVASGQLVRVVGLTLEASGCRAPVGSLCSIETMAGELIAEVVGFDDKLLYLMPIEEL
RGVLPGARVQPLGEQSGLNVGLSLLGRVLDGNGLPLDGLGALNTDQHASRHGPSINPLSRRAITEPLDVGVRAINAMLTV
GKGQRMGLFAGSGVGKSVLLGMMTRGTTADIIVVGLVGERGREVKEFIEEILGEHGRARSVVVAAPADTSPLMRLRACET
STRIAEYFRDLGYNVLLLMDSLTRYAQAQREVALAVGEPPATKGYPPSVFAKLPRLVERAGNGGPGQGSITAFYTVLTEG
DDQQDPIADASRAILDGHIVLSRRLADSGHYPAIDIESSISRVAPMVISNEHLEAMRRVKQMYSMYQQNRDLISIGAYAQ
GSDPRIDNAIRLQPAMNAFLRQGFKEAISFDDSQLMMSQIAAQCQV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-75 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-74 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-74 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-78 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-78 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-78 48
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-77 48
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-78 48
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 8e-78 48
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-78 48
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 3e-62 47
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 9e-59 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-70 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-64 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-64 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-65 47
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 8e-65 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-65 47
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-63 46
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-63 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-63 45
escN AAC38383.1 EscN Virulence LEE Protein 9e-68 45
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-67 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-68 45
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-67 45
escN CAI43874.1 EscN protein Virulence LEE Protein 8e-68 45
escN AAC31513.1 L0034 Virulence LEE Protein 8e-68 45
unnamed AAL06369.1 EscN Virulence LEE Protein 9e-66 45
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 8e-68 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-67 45
escN AAK26715.1 EscN Virulence LEE Protein 2e-67 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-67 45
escN AAL57542.1 EscN Virulence LEE Protein 2e-67 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-67 45
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-68 44
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 4e-61 44
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 4e-61 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-66 43
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 7e-63 43
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-66 43
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 6e-64 43
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-63 43
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-63 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 8e-52 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_562336.1 flagellum-specific ATP synthase VFG1254 Protein 1e-118 65
fliI YP_562336.1 flagellum-specific ATP synthase VFG2331 Protein 9e-103 64
fliI YP_562336.1 flagellum-specific ATP synthase VFG2499 Protein 6e-110 62
fliI YP_562336.1 flagellum-specific ATP synthase VFG0048 Protein 1e-74 48
fliI YP_562336.1 flagellum-specific ATP synthase VFG0192 Protein 2e-72 48
fliI YP_562336.1 flagellum-specific ATP synthase VFG2144 Protein 7e-73 47
fliI YP_562336.1 flagellum-specific ATP synthase VFG0515 Protein 1e-64 47
fliI YP_562336.1 flagellum-specific ATP synthase VFG0390 Protein 4e-69 45
fliI YP_562336.1 flagellum-specific ATP synthase VFG0730 Protein 5e-68 45
fliI YP_562336.1 flagellum-specific ATP synthase VFG0812 Protein 4e-68 45
fliI YP_562336.1 flagellum-specific ATP synthase VFG2459 Protein 6e-58 41
fliI YP_562336.1 flagellum-specific ATP synthase VFG1778 Protein 8e-55 41