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Name : lysS (Bpro_3620) Accession : YP_550423.1 Strain : Polaromonas sp. JS666 Genome accession: NC_007948 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : - Position : 3823160 - 3824710 bp Length : 1551 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGTCCATCCACCCCGCTTTGCCCGCAGCCGACAATGCGATTCCCACCCCGCCAGACGACAACCAGCTGATGGTCGAGCG GCGCGAAAAGCTCAAGGCCCTGCGCAGCGCCGGCGTCGCTTTTCCGAATGACGTCCGACCCGAGCACCGGGCCGAGGCAC TGTTTGCGCAGTACGACAGCAAGACCAAGGAAGAGCTGGGACCTTTGGCCGTGAAGGTGAGCGTCGCCGGCCGCATGATG CTCAAGCGGGTGATGGGAAAAGCCAGCTTTGCCACCCTGCAGGACGCTTCGTTTGGTCCGTCGGGTGGGCGCATCCAGGT GTACATCAACAACGAGGGCGTGGGCGAAGAAGTTCACAACGCCTTCAAGCATTGGGACCTGGGCGACATCGTCGCGGCCA CCGGTACGCTGTTCAAGACCAAGACCGGCGAGCTGTCGATTCATGCCACCAGTATCCGGCTGGTCACCAAAAGCCTGCGC CCCCTGCCCGACAAATTCCACGGCATGGCCGACCAGGAAGTCAAATACCGCCAGCGCTATGTGGACCTGATCATGGACGA GTCCGCCCGCAAGCGCTTCATGGCGCGCAGCAAGGCGGTGAGTGGCCTTCGCGAATTCATGGTGGCCCATGATTTCCTCG AGGTGGAAACTCCCATGCTGCATCCGATTCCGGGTGGCGCCAATGCCAGGCCGTTCAAGACGCATCACAACGCGCTGGAC CAGGAAATGTTCCTGCGTATCGCGCCCGAGCTTTACCTGAAACGCCTGATCGTTGGCGGCTTTGAACGTGTGTTCGAGAT CAACCGCAGCTACCGCAACGAAGGCATCAGCGTGCGGCACAACCCCGAGTTCACCATGATGGAGTTCTACGCGGCCTGGT GGAATTATTCGGACCTGATGACCTTCACCGAAGCCCTGATTCGGGACGCGGCCCAGAAGGTGACGGGCTCCCTGCAATTG AGCTATGGCGGCAAGGCGGTTGACCTGAGCCAGCCCTTTGAGCGTCTCACCATTCGCGAAGCCATTCTGAAGCACACCGA AGCCGGCGAGCACGTTGACGACATGGTGTGGCTGACCAACGCCCTGAAGAAACTCGGTGCAACCGAAGCCAAGGACCGCT TGTCCACACGCTCGCTGGCCAGCCTGCAGGTCCTGTATTTTGAGGAAACGGTCGAGGAAAAACTCTGGCAGCCAACCTTC ATCATGGAGCACCCGACCGAAATCTCGCCGCTGGCGCGCGCCAACGATGCGCGCCCCGAGGTCACGGAACGCTTTGAGCT CTACATCACCGGCCGCGAATTCGGCAATGGTTTTTCCGAGCTGAACGATGCCGAGGAGCAGGCGGCCCGCTTCAACGCGC AGGTGGCTGCCAAGGACGACGGCGACGACGAAGCCATGTTCTACGACCATGACTTCATCCGCGCCCTCGAATACGGCATG CCGCCGACCGGCGGCTGCGGCGTGGGCATAGACCGGCTGATGATGCTACTGACCGACAGCCCCAGCATTCGCGATGTGAT CTTGTTCCCCGCGCTACGCCGCGAAGCGTAA Protein sequence : MSIHPALPAADNAIPTPPDDNQLMVERREKLKALRSAGVAFPNDVRPEHRAEALFAQYDSKTKEELGPLAVKVSVAGRMM LKRVMGKASFATLQDASFGPSGGRIQVYINNEGVGEEVHNAFKHWDLGDIVAATGTLFKTKTGELSIHATSIRLVTKSLR PLPDKFHGMADQEVKYRQRYVDLIMDESARKRFMARSKAVSGLREFMVAHDFLEVETPMLHPIPGGANARPFKTHHNALD QEMFLRIAPELYLKRLIVGGFERVFEINRSYRNEGISVRHNPEFTMMEFYAAWWNYSDLMTFTEALIRDAAQKVTGSLQL SYGGKAVDLSQPFERLTIREAILKHTEAGEHVDDMVWLTNALKKLGATEAKDRLSTRSLASLQVLYFEETVEEKLWQPTF IMEHPTEISPLARANDARPEVTERFELYITGREFGNGFSELNDAEEQAARFNAQVAAKDDGDDEAMFYDHDFIRALEYGM PPTGGCGVGIDRLMMLLTDSPSIRDVILFPALRREA |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 3e-132 | 55 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 4e-80 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_550423.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 2e-132 | 55 |
