Gene Information

Name : clpB (ECH_0367)
Accession : YP_507187.1
Strain : Ehrlichia chaffeensis Arkansas
Genome accession: NC_007799
Putative virulence/resistance : Virulence
Product : ATP-dependent Clp protease, ATP-binding subunit ClpB
Function : -
COG functional category : O : Posttranslational modification, protein turnover, chaperones
COG ID : COG0542
EC number : -
Position : 357222 - 359795 bp
Length : 2574 bp
Strand : +
Note : identified by similarity to SP:P03815; match to protein family HMM PF00004; match to protein family HMM PF02861; match to protein family HMM PF07724

DNA sequence :
ATGGATCTCAATCAATTTACTGATATGTCAAAGAATTTAATAATGCAGGCGCAAACTACAGCAATTGCATCAGGGCATCA
ATCTTTGATACCAGAGCATTTACTTAAAGTAATGTTAGATACTAAAGATGAGCTTATTGAGCTGTTGCTGACTTCATGTG
GATGTGATATTGATAAGATATACAGTGATATAAAACTCTCCTTAAGTAAATTACCTGTTGTAAGTGGAAGTGGTAGTGGT
CATATACATTTATCAAAAGAAATGGCTCAGGTTTTAGAAGAAGCTATTAGTTTAGCAAAAAGGAATCAGGATACTTATGT
AACTGTGGAGAGATTATTACAAGCTCTTGCAGTTGTTAAAGACACTAGTGTTTACAAGATACTACTTGCACATGGAGTAA
CTCCTGTTAAGTTAGAATCACTAATACTTAATATGCGTAATGGATCTAAGGCTGATACTATTAATGCTGAACATAAATTT
AATGCTCTGAAAAAATATGCTAAAGATATTACAGAATCTGCCATGGCTGGGAAACTTGATCCAGTTATTGGAAGAGATGA
AGAGATTAGACGTACAATGCAAGTTTTATCACGCAGAACAAAGAATAATCCTGTATTAATTGGAGAACCTGGAGTTGGTA
AGACAGCAATTATTGAAGGATTGGCACAGCGTATTGTGGTAGGTGATGTTCCTGTTGGACTAAGAAATGCAAAAATAATG
GCACTGGATCTTGGAATGCTTGTTGCTGGAACAAAATATAGAGGGGAATTTGAGGAAAGATTAAAATATGTAATTAATGA
AATAGTGGCGTCAAATGGTGCGGTTATATTGTTTATTGATGAATTACACACATTAGTTGGAGCTGGTGCTACAGATGGTG
CAATGGATGCATCGAACTTGTTAAAACCTGCTTTAGCAAGGGGTGAAATACATTGTATAGGAGCAACAACATTGGATGAA
TATAGGCAACACATAGAAAAAGATGCTGCATTGGCTAGAAGGTTTCAACCTGTGTTTGTTTCTGAATCTACTGTTAATGA
TACTATCTCTATTTTGCGTGGATTGAAAGAAAAATATGAAGTACACCATGGTATTAGGATTATGGATAGTGCGATTATTG
CTGCTTCAACTTTGTCAAATCGTTATATTACAGATAGGTTTTTACCAGATAAAGCTATAGATCTAATTGATGAAGCAGCA
AGTCGTGTAAGAATAGAGATTGATAGTAAACCTGAGGTAATTGACGAATTAGATAGAAAAATTATTCAATTGAAGATTGA
GGCTGGAGTTTTAGAAAAAGAAAATACTGAATCTTCAAAACAGCGTTTAGCCCAATTAAGTGAAGAATTGAATAAATTAA
GTATTCAAGCAACAGAATTAAATAGTAAGTGGCAGGCTGAGAAAATGAAGATATTAAAAATGCAAGAATGTGTGGAAAAG
TTAGATAATGCAAGAAATGATTTAGAAAAAGCTCAAAGGTCTGGTAATTTGGCAAAAGCTGGCGAATTGATGTATGGAAT
TATCCCAGAACTTGAAAAAGAGTTAAAAAAATGTGAAAAACCTAGTAGTAACATGTTGAAAAGGGAAGTGACTGAAAGTG
ATATAGCAAGTATTGTATCACGGTGGACAGGTATTCCTATTGAAAACATGATGAGCAGTGAAAAAGAAAAATTGCTGCGT
ATGGAAGAAGAGATAGGGAAAACAGTCATTGGTCAAGAAAGTGCTATTAAAGCAGTAAGTGATGCTGTTAGAAGATCGCG
TGCAGGAGTACAAGATGCCAATAAACCATTAGGATCTTTTTTATTCCTTGGACCTACTGGAGTAGGAAAAACAGAATTAG
TAAAAACATTAGCTGAATTTTTATTTTGTGATAAATCTGCTTTATTGAGGTTTGATATGTCTGAGTTTATGGAAAAGCAT
GCTGTATCTCGGTTAATAGGTGCTCCTCCAGGATATGTAGGATATGATCAAGGTGGTATGTTGACTGAATCTGTGAGGAG
GAGACCTTATCAAGTTATACTTTTTGATGAAATAGAGAAAGCACATGGAGATATTTTTAATATTTTATTGCAAGTATTAG
ATGAGGGACGGTTAACTGATAACCATGGTAAATTGGTTGATTTTCGTAATACAATACTGGTTTTAACATCAAACTTAGGA
CAAGAAATACTGATTAATAATAAGGAAGATGTTGATGGAGAGTCAGTAAAAAAATCTATAACTAGTGTGTTACAACATCA
TTTTCGGCCAGAATTTTTAAACAGATTAGATGAAATTATTGTATTTCATAGATTAACTAAGGAACATATAGAGAAAATTA
TAGATGTACAATTTTCTCTGTTACAGAAAATTGTTGCTCAAAAAGAGTTAGAGATTAGTTTATCTTCGGAGGCAAAATCT
TGGTTGATGAATAATGGCTATGATTCGTTATATGGGGCAAGACCTTTGAAAAGATTAATACAACAAAAAATACAAAATAG
TTTAGCAAAGTTAATACTTGCAAATCAAGTTAGTAAGGGAGATAAGCTAGAAGTAGTTTTGTTGAATGACGATTTAATTA
TTAATAAGTTATAG

Protein sequence :
MDLNQFTDMSKNLIMQAQTTAIASGHQSLIPEHLLKVMLDTKDELIELLLTSCGCDIDKIYSDIKLSLSKLPVVSGSGSG
HIHLSKEMAQVLEEAISLAKRNQDTYVTVERLLQALAVVKDTSVYKILLAHGVTPVKLESLILNMRNGSKADTINAEHKF
NALKKYAKDITESAMAGKLDPVIGRDEEIRRTMQVLSRRTKNNPVLIGEPGVGKTAIIEGLAQRIVVGDVPVGLRNAKIM
ALDLGMLVAGTKYRGEFEERLKYVINEIVASNGAVILFIDELHTLVGAGATDGAMDASNLLKPALARGEIHCIGATTLDE
YRQHIEKDAALARRFQPVFVSESTVNDTISILRGLKEKYEVHHGIRIMDSAIIAASTLSNRYITDRFLPDKAIDLIDEAA
SRVRIEIDSKPEVIDELDRKIIQLKIEAGVLEKENTESSKQRLAQLSEELNKLSIQATELNSKWQAEKMKILKMQECVEK
LDNARNDLEKAQRSGNLAKAGELMYGIIPELEKELKKCEKPSSNMLKREVTESDIASIVSRWTGIPIENMMSSEKEKLLR
MEEEIGKTVIGQESAIKAVSDAVRRSRAGVQDANKPLGSFLFLGPTGVGKTELVKTLAEFLFCDKSALLRFDMSEFMEKH
AVSRLIGAPPGYVGYDQGGMLTESVRRRPYQVILFDEIEKAHGDIFNILLQVLDEGRLTDNHGKLVDFRNTILVLTSNLG
QEILINNKEDVDGESVKKSITSVLQHHFRPEFLNRLDEIIVFHRLTKEHIEKIIDVQFSLLQKIVAQKELEISLSSEAKS
WLMNNGYDSLYGARPLKRLIQQKIQNSLAKLILANQVSKGDKLEVVLLNDDLIINKL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
clpC YP_005163377.1 ATP-dependent Clp protease ATP-binding subunit Not tested Not named Protein 2e-167 43
STY0294 NP_454876.1 ClpB-like protein Not tested SPI-6 Protein 3e-100 41
aec27 YP_851418.1 ATPase Not tested PAI II APEC-O1 Protein 6e-105 41
aec27 AAQ96721.1 Aec27 Not tested AGI-1 Protein 4e-105 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
clpB YP_507187.1 ATP-dependent Clp protease, ATP-binding subunit ClpB VFG2076 Protein 6e-112 41