Gene Information

Name : lysS (RHE_CH03657)
Accession : YP_471139.1
Strain : Rhizobium etli CFN 42
Genome accession: NC_007761
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3872861 - 3874357 bp
Length : 1497 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
GTGGCTGACAACAAGACCGAAAACACCCTTTCCTCCGACGCGACCGAGGTGCGCGCCCAGAAGCTGAAGCTGCTGCGCGA
GCAGATCGGCGATGTCTATCCGGCGCATTTTCACCGGAGCCTGACCAATGCCGAGCTTGCGACGAGATACGAGGGTCTGG
ACCCCGATGTCGAGACGCAGGACATCGTCACCGTCGCTGGCCGGGTCTATTCCTCGCGCAACTCGGGCATGTTCATGGAT
ATTCATGACGCCTCCGGCAAGATCCAGATCTTTTCGCATAAGGACGTGACAGGCGAGGAAGCCCGCGCCCTGCTGCCGAT
GATCGACATTGGCGACATCATCGGCGTCACCGGCATCGTGCGCCGCACCAAGCGCGGTGAACTGACGATCAACGCCCAGA
AGATCGAGATGCTGACCAAGTCGCTGCTGCCGATGCCGGAGAAGTGGCACGGCCTTTCCGACATCGAGCTGCGCTACCGC
AAGCGCCATCTCGATATTTTGACCAACGAGGATTCGAAGCTGCGCTTCCAGCAGCGCTCGAAGATCGTCTCCGGCATCCG
CCGCTTCATGGAAAATGACGGCTTCATGGAAGTCGAGACGCCGATGCTGCAGTCGATCTATGGCGGCGCCACCGCCGAGC
CGTTCAAGACGCATCACAACACGCTGAAGCTCGACATGTACCTGCGCATTGCGCCGGAACTGTTCTTGAAGCGCACGCTG
GTCTCCGGCCTCACCGACAAGGTGTTTGAAATCAACCGCAACTTCCGCAACGAAGGCGTCTCCACCCGGCACAATCCGGA
ATTCACCATGATGGAGTGCTACTGGGCCTATGCGGATTACGAGGACATGATGGACCTCGTCGAGCGTCTGTTCGAGACCT
TGGCTCTCTCCATTCACGGCACGACGGAATTCGACTTCGGCGACAAGCGGCTGTCCTTCAAGGGACCATTCAAGCGCGTG
CCGATGCCCGACGCCGTCAAGGAAGTAACCGGCATCGATTTCCTCGCCATCAAGACCGATGAGGAAGCGCGCGCCGCGGC
CAAGGCTGCCGGCTTTGCAGTCGAGAAGGATTGGACCTGGGGCGAGTGCCTCGCCTTCATCTTCGAGGAAAAGGTCGAAG
CCACGCTGATTCAGCCGTCTCACGTCACCCATTTCCCGAAGGACATTTCGCCCTTCGCCAAGGAAGTGCCGGGCGAGCCG
CGCCTCGTCGAGCGCTTCGAGACCTATTGCAACGCCTGGGAACTCGGCAACGCCTTCTCGGAACTCAACGATCCCGAGGA
GCAGCGGCGCCGCATGGTCGAGCAGCTCGAACAGGCGCATGCCCGCGGCGAAAAGGAAAAGCAGCTGGACGAGGAATTCC
TCGACGCAATCGACCAGGGCATGCCGCCGGCCGGCGGCCTCGGCATCGGCGTCGACCGTCTGATCATGCTGCTGACCAAC
GCTCCGTCGATCCGCGACGTCATTCTCTTCCCGGCCCGCCGCAGCAAGGCTGACTAA

Protein sequence :
MADNKTENTLSSDATEVRAQKLKLLREQIGDVYPAHFHRSLTNAELATRYEGLDPDVETQDIVTVAGRVYSSRNSGMFMD
IHDASGKIQIFSHKDVTGEEARALLPMIDIGDIIGVTGIVRRTKRGELTINAQKIEMLTKSLLPMPEKWHGLSDIELRYR
KRHLDILTNEDSKLRFQQRSKIVSGIRRFMENDGFMEVETPMLQSIYGGATAEPFKTHHNTLKLDMYLRIAPELFLKRTL
VSGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDMMDLVERLFETLALSIHGTTEFDFGDKRLSFKGPFKRV
PMPDAVKEVTGIDFLAIKTDEEARAAAKAAGFAVEKDWTWGECLAFIFEEKVEATLIQPSHVTHFPKDISPFAKEVPGEP
RLVERFETYCNAWELGNAFSELNDPEEQRRRMVEQLEQAHARGEKEKQLDEEFLDAIDQGMPPAGGLGIGVDRLIMLLTN
APSIRDVILFPARRSKAD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-95 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_471139.1 lysyl-tRNA synthetase VFG1668 Protein 2e-95 47