Gene Information

Name : lysS (MCAP_0836)
Accession : YP_424778.1
Strain : Mycoplasma capricolum ATCC 27343
Genome accession: NC_007633
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 966562 - 968064 bp
Length : 1503 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTTAGACGATAGAAAATTTAGTGAACAAGAATTAGTTAGAAGAAATAAATATAAAAATTTAGTTGATCAAAATAAAGA
TCCTTATAAAATAACAAACTGAAAAAGAAACACTACATTATTAAAGTTAAATGAAAAATATAAAGATTATAGTAAAGAAG
AGTTATTAAATTTAACTCAAGAACTAGTTATTGTAGCTGGAAGAATTAAGTTATATAGAGAAGCTGGAAAAAAAGCTGCA
TTTGTTAATATTGATGATCAAGATTCAAGTATTCAATTATATGTAAGATTAGATGAAATAGGTCAAGAAGCTTTTGAAGA
TTTTAGGGATTTAGATCTTGGAGATATTATTGGAGTAAAAGGGAACATGATGAGAACAGATCATGGTGAATTATCAATTA
GATGTAAAGAGGTTGTTTTACTATCTAAAGCTTTAAGACCTTTACCTGATAAACATGCTGGAATTCAAGATATAGAAGAA
AAATATCGTAGAAGATATGTTGATCTAATTATGAATCATGACGTTAGAAAAACATTTCAAGCTAGAACTAAAATTATAAG
AACAATGCAAAATTTTTTAGATAATAGAGGTTATATGGAAGTTGAGACTCCTATTTTGCATTCACTAAAAGGTGGAGCTT
CTGCTAAACCGTTTGTTACTCATTATAATGTTTTAAATACTGATGTTTATTTAAGAATAGCAACAGAATTACACTTAAAA
CGTTTAATTGTTGGTGGTTTTGAAGGAGTTTATGAAATAGGACGTATTTTTAGAAATGAAGGTATGAGCACAAGACACAA
CCCTGAATTTACAAGTATTGAGTTATATGTTGCTTATGAAGATATGTTCTTTTTAATGGACTTAACTGAAGAAATTTTTA
GAGTATGTAATTCAGCGGTAAATTCAAGTAGTGTAATAGAATATAACAACGTTAAAATTGATTTATCAAAACCTTTTAAA
AGATTACATATGGTTGATGGAATTAAACAAGTTACAGGTGTTGATTTTTGACAAGAAATGACTGTAGAACAAGCCTTAGA
ATTAGCTAAAAAACATAATGTTTATGTTGAAAAACACCAAGAATCAGTTGGGCATATAATTAACTTATTTTATGAAGAAT
TTGTTGAATCAACTATTGTTGAACCTACATTCGTTTATGGTCATCCAAAAGAAATTTCTCCATTAGCAAAATCAAATCCA
AATGATCCAAGATTTACTGATAGATTTGAATTATTTATTATTGGTAGAGAATATGCTAATGCTTTTAGTGAGTTAAATGA
TCCAATTGATCAGTATGAGAGATTCAAGGCTCAACTAGAAGAAGAATCTAAAGGAAATGACGAAGCAAATGATATGGATA
TTGATTTTGTTGAAGCTTTAGAACATGCGATGCCACCAACTGCGGGTATTGGTATTGGAATTGATAGATTAGTTATGCTT
TTAACTAATTGTGACTCTATTAAAGATGTTTTGCTATTTCCACAAATGAAACCAAAAGAATAA

Protein sequence :
MLDDRKFSEQELVRRNKYKNLVDQNKDPYKITNWKRNTTLLKLNEKYKDYSKEELLNLTQELVIVAGRIKLYREAGKKAA
FVNIDDQDSSIQLYVRLDEIGQEAFEDFRDLDLGDIIGVKGNMMRTDHGELSIRCKEVVLLSKALRPLPDKHAGIQDIEE
KYRRRYVDLIMNHDVRKTFQARTKIIRTMQNFLDNRGYMEVETPILHSLKGGASAKPFVTHYNVLNTDVYLRIATELHLK
RLIVGGFEGVYEIGRIFRNEGMSTRHNPEFTSIELYVAYEDMFFLMDLTEEIFRVCNSAVNSSSVIEYNNVKIDLSKPFK
RLHMVDGIKQVTGVDFWQEMTVEQALELAKKHNVYVEKHQESVGHIINLFYEEFVESTIVEPTFVYGHPKEISPLAKSNP
NDPRFTDRFELFIIGREYANAFSELNDPIDQYERFKAQLEEESKGNDEANDMDIDFVEALEHAMPPTAGIGIGIDRLVML
LTNCDSIKDVLLFPQMKPKE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-102 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_424778.1 lysyl-tRNA synthetase VFG1668 Protein 6e-103 46