Gene Information

Name : SBO_1547 (SBO_1547)
Accession : YP_407990.2
Strain : Shigella boydii Sb227
Genome accession: NC_007613
Putative virulence/resistance : Unknown
Product : insertion element IS2 transposase InsD
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG2801
EC number : -
Position : 1529028 - 1529936 bp
Length : 909 bp
Strand : +
Note : IS2 OrfB; forms an overlapping reading frame with orfA; may form a fusion protein OrfAB due to ribosomal frameshifting; in some cases OrfB is itself expressed; both OrfA and OrfAB bind terminal inverted repeats

DNA sequence :
GTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAAGCTTAGTCAGCCGTTGTCTCCGGGTGTCGCGTGCGCA
GTTGCACGTCATTCTCAGACGAACCGATGACTGGATGGATGGCCGCCGCAGTCGTCACACTGATGATACGGATGTGCTTC
TCCGTATACACCATGTTATCGGAGAGCTGCCCACGTATGGTTATCGTCGGGTATGGGCGCTGCTTCGCAGACAGGCAGAA
CTTGATGGTATGCCAGCGATCAATGCCAAACGTGTTTACCGGATCATGCGCCAGAATGCGCTGTTGCTTGAGCGAAAGCC
TGCTGTACCGCCATCGAAACGGGCACATACAGGCAGAGTGGCCGTGAAAGAAAGCAATCAGCGATGGTGCTCTGACGGGT
TCGAGTTCTGCTGTGATAACGGAGAGAGACTGCGTGTCACGTTCGCGCTGGACTGCTGTGATCGTGAGGCACTGCACTGG
GCGGTGACTACCGGCGGCTTCAACAGTGAAACAGTACAGGACGTCATGCTGGGAGCAGTGGAACGCCGCTTCGGCAACGA
TCTTCCGTCGTCTCCAGTGGAGTGGCTGACGGATAATGGTTCATGCTACCGGGCTAATGAAACACGCCAGTTCGCCCGGA
TGTTGGGACTTGAACCGAAGAACACGGCGGTGCGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATA
AAGCGTGACTACATCAGTATCATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAACA
TTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGT
TAAGTGATAACAGATGTCTGGAAATATAG

Protein sequence :
MDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAE
LDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHW
AVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTI
KRDYISIIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
tnpF AAD44739.1 TnpF Not tested SHI-2 Protein 1e-120 99
S3189 NP_838472.2 insertion element IS2 transposase InsD Not tested SHI-1 Protein 4e-137 99
S4050 NP_839218.2 insertion element IS2 transposase InsD Not tested SHI-2 Protein 3e-138 99
SF3708 NP_709447.1 IS2 transposase TnpB Not tested SHI-2 Protein 3e-138 99
SF3721 NP_709460.1 IS2 transposase TnpB Not tested SHI-2 Protein 3e-138 99
S4061 NP_839230.2 insertion element IS2 transposase InsD Not tested SHI-2 Protein 1e-138 99
SF2985 NP_708759.1 insertion element IS2 transposase InsD Not tested SHI-1 Protein 1e-118 99
insD YP_002414033.1 insertion element IS2 transposase InsD Not tested Not named Protein 3e-135 98
unnamed CAC39284.1 hypothetical protein Not tested LPA Protein 5e-118 97
unnamed AAL67379.1 transposase-like protein Not tested PAI II CFT073 Protein 1e-117 97
ECO111_3773 YP_003236109.1 putative IS2 transposase InsAB Not tested LEE Protein 2e-119 94
aec49 AAW51732.1 Aec49 Not tested AGI-3 Protein 2e-120 84
unnamed CAC39283.1 hypothetical protein Not tested LPA Protein 1e-82 63
c5197 NP_757045.1 transposase insD Not tested PAI II CFT073 Protein 1e-61 60
insD YP_854263.1 IS2 transposase Not tested PAI I APEC-O1 Protein 2e-65 60
insD AAZ04432.1 IS2 transposase Not tested PAI I APEC-O1 Protein 2e-65 60

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
SBO_1547 YP_407990.2 insertion element IS2 transposase InsD VFG0621 Protein 1e-138 99
SBO_1547 YP_407990.2 insertion element IS2 transposase InsD VFG0608 Protein 1e-138 99
SBO_1547 YP_407990.2 insertion element IS2 transposase InsD VFG0649 Protein 5e-119 99
SBO_1547 YP_407990.2 insertion element IS2 transposase InsD VFG1732 Protein 1e-135 98