Gene Information

Name : SDY_4229 (SDY_4229)
Accession : YP_405622.1
Strain : Shigella dysenteriae Sd197
Genome accession: NC_007606
Putative virulence/resistance : Unknown
Product : insertion sequence 2 OrfA protein
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG2963
EC number : -
Position : 3962369 - 3962734 bp
Length : 366 bp
Strand : -
Note : IS2 OrfA; forms an overlapping reading frame with orfB; may form a fusion protein OrfAB due to ribosomal frameshifting; both OrfA and OrfAB bind terminal inverted repeats

DNA sequence :
ATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGA
ACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATACC
AGGAAGGAAGTCTTACTGCTGTGGCCGCCGGAGAACAGGTTGTTCCTGCCTCTGAACTTGCTGCCGCCATGAAGCATATT
AAAGAACTCCAGCGCCTGCTCGGCAAGAAAACGATGGAAAATGAACTCCTCAAAGAAGCCGTTGAATATGGACGGGCAAA
AAAGTGGATAGCGCACGCGCCCTTATTGCCCGGGGATGGGGAGTAA

Protein sequence :
MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKHI
KELQRLLGKKTMENELLKEAVEYGRAKKWIAHAPLLPGDGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
S4049 NP_839217.2 insertion sequence 2 OrfA protein Not tested SHI-2 Protein 6e-52 99
tnpG AAD44740.1 TnpG Not tested SHI-2 Protein 1e-52 99
unnamed AAL57520.1 IS2 transposase Not tested LEE Protein 1e-52 99
ECO26_5247 YP_003232129.1 insertion sequence 2 OrfA protein Not tested LEE Protein 2e-52 99
st02 CAC81840.1 ST02 protein Not tested LEE II Protein 1e-52 99
S3188 NP_838471.2 insertion sequence 2 OrfA protein Not tested SHI-1 Protein 2e-52 99
S4060 NP_839229.2 insertion sequence 2 OrfA protein Not tested SHI-2 Protein 2e-52 99
SF3709 NP_709448.3 IS2 ORF1 Not tested SHI-2 Protein 7e-52 99
SF3722 NP_709461.3 IS2 ORF1 Not tested SHI-2 Protein 7e-52 99
aec50 AAW51733.1 Aec50 Not tested AGI-3 Protein 2e-52 99
SF2984 NP_708758.3 IS2 ORF1 Not tested SHI-1 Protein 2e-52 99
insC YP_002414034.1 IS2 insertion element repressor InsA; KpLE2 phage-like element Not tested Not named Protein 2e-36 98
unnamed AAL67376.1 insertion sequence protein Not tested PAI II CFT073 Protein 7e-37 88
insC YP_854262.1 IS2 transposase Not tested PAI I APEC-O1 Protein 3e-30 76
c5196 NP_757044.1 transposase insC Not tested PAI II CFT073 Protein 3e-30 76
insC AAZ04431.1 IS2 conserved hypothetical protein Not tested PAI I APEC-O1 Protein 2e-30 76

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
SDY_4229 YP_405622.1 insertion sequence 2 OrfA protein VFG0609 Protein 2e-52 99
SDY_4229 YP_405622.1 insertion sequence 2 OrfA protein VFG0648 Protein 6e-53 99
SDY_4229 YP_405622.1 insertion sequence 2 OrfA protein VFG0622 Protein 2e-52 99
SDY_4229 YP_405622.1 insertion sequence 2 OrfA protein VFG1733 Protein 6e-37 98