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Name : LSA0084 (LSA0084) Accession : YP_394695.1 Strain : Lactobacillus sakei 23K Genome accession: NC_007576 Putative virulence/resistance : Unknown Product : rRNA large subunit methyltransferase Function : - COG functional category : S : Function unknown COG ID : COG1576 EC number : - Position : 79985 - 80464 bp Length : 480 bp Strand : + Note : SPOUT methyltransferase; crystal structure shows homodimer; in Escherichia coli this protein methylates pseudouridine at position 1915 of the 23S ribosomal RNA DNA sequence : ATGAATATCAAAATTATCACAGTTGGGAAACTGAAAGAAAAATACTTGAAGGCGGGCATTGCGGAATACGCCAAACGCTT AAGTAAATTTTGTAAGTTTGAAATCATCGAAGTGTCAGACGAAAAGGCACCGGAATCTTTGAGCGAGGCTGAAATGACGA CCGTTAAAGATAAAGAAGGCGAACGGATCTTAGCCAAGGTGAAGGATAAGGAACACGTGATTGTGTTAGCCATTCACGGC AAACAACGTGCTTCAGAAGAATTCGCAAAAGAAATCCAAGATTTGGCAACCTATGGCACATCAGATATTACCTTCATTAT TGGGGGCTCACTCGGAACAAGTGATGCCGTTAATAAACGCGCTAACGACGCCTTATCGTTTGGCAAATTGACGTTACCAC ACCAATTGATGCGCTTAGTTCTAACGGAACAAATCTACCGGGCGTTTATGATTAACCAGGGATCGCCGTATCATAAATAA Protein sequence : MNIKIITVGKLKEKYLKAGIAEYAKRLSKFCKFEIIEVSDEKAPESLSEAEMTTVKDKEGERILAKVKDKEHVIVLAIHG KQRASEEFAKEIQDLATYGTSDITFIIGGSLGTSDAVNKRANDALSFGKLTLPHQLMRLVLTEQIYRAFMINQGSPYHK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| SSP0026 | YP_300116.1 | rRNA large subunit methyltransferase | Not tested | SCC15305RM | Protein | 2e-41 | 60 |
| orfX | YP_251938.1 | rRNA large subunit methyltransferase | Not tested | SCCmec | Protein | 4e-41 | 58 |
| unnamed | BAC53844.1 | open reading frame X | Not tested | SRImec-III and SCCmec-III region | Protein | 9e-41 | 57 |
| SAV0024 | NP_370548.1 | rRNA large subunit methyltransferase | Not tested | Type-II SCCmec | Protein | 1e-40 | 57 |
| unnamed | BAC57492.1 | hypothetical protein | Not tested | Type-IIIinv SCCmec | Protein | 9e-41 | 57 |
| unnamed | BAB72121.1 | hypothetical protein | Not tested | Type-IVa SCCmec | Protein | 9e-41 | 57 |
| unnamed | BAB72138.1 | open reading frame X | Not tested | Type-IVb SCCmec | Protein | 9e-41 | 57 |
| unnamed | BAC67575.1 | open reading frame X | Not tested | Type-IVc SCCmec | Protein | 9e-41 | 57 |
| orfXhom | BAB83497.1 | open reading frame X | Not tested | SCC 12263 | Protein | 5e-38 | 57 |
| unnamed | BAA82243.1 | orfX | Not tested | Type-II SCCmec | Protein | 9e-41 | 57 |
| SE0023 | NP_763578.1 | rRNA large subunit methyltransferase | Not tested | SCCpbp4 | Protein | 2e-40 | 57 |
| unnamed | BAD24821.1 | conserved hypothetical protein orfX | Not tested | Type-V SCCmec | Protein | 9e-41 | 57 |
| orfX | BAA86653.1 | open reading frame X | Not tested | Type-I SCCmec | Protein | 9e-41 | 57 |
| SERP2529 | YP_190070.1 | rRNA large subunit methyltransferase | Not tested | Type-II SCCmec | Protein | 2e-40 | 57 |
| unnamed | BAG06184.1 | conserved hypothetical protein orfX | Not tested | Type-VII SCCmec | Protein | 9e-41 | 57 |
| unnamed | BAB47681.1 | open reading frame X | Not tested | Type-III SCCmec | Protein | 9e-41 | 57 |
| SAR0024 | YP_039501.1 | rRNA large subunit methyltransferase | Not tested | Type-II SCCmec | Protein | 1e-40 | 57 |
| orfX | AAL26648.1 | unknown | Not tested | SCCcap1 | Protein | 1e-40 | 56 |
| unnamed | ACL99851.1 | hypothetical protein | Not tested | Type-V SCCmec | Protein | 2e-24 | 53 |
