Gene Information

Name : lysS (Tcr_0726)
Accession : YP_390996.1
Strain : Thiomicrospira crunogena XCL-2
Genome accession: NC_007520
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 797526 - 799040 bp
Length : 1515 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGCAAACCCTGAAAACCCAACTCCTGAAGTCGATGAAAATAAAATCATTGCGGAACGACGTGCAAAATTAGCGGCTCT
GCGTGAAGCCGGTCACTCTTACCCAAATACGTTTAGACGTAACGATGTGTCGAGTGACTTGCATGAGCAATACGATGCCT
TCAGTAAAGAAGAACTGGCTGAGATGGAACCTGTTCGTGTCAAAGTCGCCGGTCGTATGTTATTGCGTCGTATTATGGGG
AAAGCCAGTTTTGCGACATTGCAGGATACACATGGTCGTATTCAGATTTACGTGACACGTGATGAGTTGCCTGAAGGGTT
TTACAATACGCAATTCAAAAAATGGGACTTAGGGGATATTGTTGGTGCGGAAGGGTATCTTTTCAAAACCAATACTGGCG
AGTTGTCTGTTCATGTTGAACATATTGAATTGATTACCAAATCGTTGCGTCCTTTACCTGATAAATTCCACGGGTTACAA
GATCAGGAAATCAAATATCGTCAGCGTTATGTGGATTTGATCGTGAACGAAGAAAGTCGTGACACTTTCATGATCCGTTC
AAAAATCGTACAGCATGTCCGTAACTTTTTAATTAATAAAGGTTTTATGGAAGTTGAAACGCCGATGATGCATGTCATTC
CGGGTGGGGCGACCGCCAAACCATTCCATACGCATCACAATGCTTTAGACATGCCACTTTATTTGCGAATTGCGCCTGAG
CTTTACCTGAAGCGTTTGGTTGTGGGTGGGTTTGAAAAGGTTTTCGAAATCAATCGAAGCTTCCGTAATGAAGGCTTGTC
GACTCGCCATAACCCAGAGTTCACCATGGTTGAGTTCTATTCGGCCTACACCGATTACAATCAATTGATGGATTACACGG
AAGAATTGCTAAAAGAATTGGCAGAACTGACGGTTGGCTCTTCTCAGGTACCTTATCAAGGTCAAGTGTTTGATTTCGGG
AAACCCTTTGCGCGCCTAACGATGAAAGATGCGATTTTGAAATACAACCCAAGCGCGACGGCTGAAAATCTAGACGACTT
AGAGTCAGCACGTGCATTGGCGAAATCGTTGAATGTGCATGTGGAAGAGAGTTATGGATTAGGCAAAATCTGGACGGAAA
TTTTCGAAGAAACGGCGGAAGAAAAACTGATGGACCCTACGTTCATTACTGAGTATCCGGCAGAAGTGTCACCATTGGCG
CGTCGTAATGACGATAATCCGTTTATTACCGATCGTTTTGAATTGTTCATTGGCGGGCGAGAGTTGGCGAATGGCTTTAA
CGAGTTGAATGATGCGGAAGATCAAGCTGAACGCTTTAAGGCGCAGGTTGAAGCGAAGGATGCCGGAGATGATGAAGCCA
TGCATTATGATGAAGATTACATTGTGGCGCTTGAGCACGGTATGCCGCCAACAGCAGGGGAAGGAATCGGGATTGACCGC
TTGGTGATGTTGTTTACCGACAAACCAACCATTCGCGATGTCTTGTTGTTCCCGCATATGAGACAAGCCGATTAA

Protein sequence :
MANPENPTPEVDENKIIAERRAKLAALREAGHSYPNTFRRNDVSSDLHEQYDAFSKEELAEMEPVRVKVAGRMLLRRIMG
KASFATLQDTHGRIQIYVTRDELPEGFYNTQFKKWDLGDIVGAEGYLFKTNTGELSVHVEHIELITKSLRPLPDKFHGLQ
DQEIKYRQRYVDLIVNEESRDTFMIRSKIVQHVRNFLINKGFMEVETPMMHVIPGGATAKPFHTHHNALDMPLYLRIAPE
LYLKRLVVGGFEKVFEINRSFRNEGLSTRHNPEFTMVEFYSAYTDYNQLMDYTEELLKELAELTVGSSQVPYQGQVFDFG
KPFARLTMKDAILKYNPSATAENLDDLESARALAKSLNVHVEESYGLGKIWTEIFEETAEEKLMDPTFITEYPAEVSPLA
RRNDDNPFITDRFELFIGGRELANGFNELNDAEDQAERFKAQVEAKDAGDDEAMHYDEDYIVALEHGMPPTAGEGIGIDR
LVMLFTDKPTIRDVLLFPHMRQAD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-141 60
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 3e-80 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_390996.1 lysyl-tRNA synthetase VFG1668 Protein 9e-142 60