Gene Information

Name : murD (Pfl01_4675)
Accession : YP_350403.1
Strain : Pseudomonas fluorescens Pf0-1
Genome accession: NC_007492
Putative virulence/resistance : Unknown
Product : UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG0771
EC number : 6.3.2.9
Position : 5277432 - 5278778 bp
Length : 1347 bp
Strand : -
Note : UDP-N-acetylmuramoylalanine--D-glutamate ligase; involved in peptidoglycan biosynthesis; cytoplasmic; catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine during cell wall formation

DNA sequence :
GTGTCTCTGATCGCTTCTGACCACTTCCGCATCGTTGTCGGCCTCGGCAAGAGCGGCATGTCCCTGGTTCGCTTCCTGGC
GAACCGGGGCGTGGCGTTTGCCGTGGCCGATACGCGGGAAAATCCACCGGAACTGGCCACGCTCCAGCGTGACTATCCGC
ACGTGGAAGTGCGTTGTGGCGAGCTGGACGTCGAGTTCCTGTGCCGTGCCGACGAGCTCTACGTGAGCCCCGGCTTGGCG
CTGGCGACCCCGGCCCTGCAAGCCGCCGCGGCCCGTGGCGTGAAGCTGTCCGGCGACATCGAGCTGTTCGCGCGTAACGC
GAAGGCGCCGATCGTGGCCATCAGCGGTTCCAACGCGAAAAGCACCGTCACCACCTTGGTCGGCGAGATGGCGGCTGCGG
CCGGCAAGCGCGTGGCCGTGGGCGGCAACCTCGGTACGCCGGCGCTGGATCTGCTCAGCGATGACGTCGAGCTGTACGTG
ATGGAACTGTCGAGTTTCCAGCTGGAAACCACCGACCAGCTCAACGCCGAAGTGGCGACCGTGCTCAACATCAGCGAAGA
CCACATGGACCGCTACAGCGGTCTGCCGGCGTATCACCTGGCCAAACACCGGATCTTCCGTGGCGCCAAGCAGTTCGTGG
TCAACCGTCAGGATGCGTTGACCCGTCCGTTGATGGGCGAGGGCCAGCCGTGCTGGACCTTCGGCCTGAGCAAACCGGAT
TTCAAGGCGTTCGGCCTGCGTGAAGAGAATGGCGAGAAATACCTGGCCTTCGAATTCCAGAACCTGATGCCGGTGCGCGA
ACTGAAGATTCGCGGCGCCCACAACCAGTCCAACGCCCTGGCGGCGCTGGCACTCGGCCACGCGGTCGGGCTGCCGTTCG
ACGCCATGCTGGTGGCGCTGCGCACCTTCGCCGGTCTCGAACACCGTTGTCAGTGGGTGCGTGATCTGGACGGCGTCGGT
TACTACAACGATTCCAAAGCCACCAACGTGGGCGCCGCACTGGCCGCCATCGAAGGCCTGGGTGCCGACATCGACGGCAA
GGTCGTGCTGATCGCCGGTGGCGACGGCAAGGGCGCCGAATTCAAGGACCTGCGCGATCCGGTGGCGACCCATTGCCGCG
CCGTGATCCTGATGGGCCGTGACTCCGACAAGATCGGCGAAGCGATCGGCGATGCCGTTCCGCTGATTCGCGCCACCACG
CTGGTCGATGCGGTCGCCCAGTGCCGCGCCGCCGCCCAGCCGGGAGACGTGGTGCTGCTGTCGCCGGCCTGCGCCAGTTT
CGACATGTTCAATAACTACGAAGACCGTGGTCACCAGTTCGTCCGCGCCGTGGAGGATCTGGCATGA

Protein sequence :
MSLIASDHFRIVVGLGKSGMSLVRFLANRGVAFAVADTRENPPELATLQRDYPHVEVRCGELDVEFLCRADELYVSPGLA
LATPALQAAAARGVKLSGDIELFARNAKAPIVAISGSNAKSTVTTLVGEMAAAAGKRVAVGGNLGTPALDLLSDDVELYV
MELSSFQLETTDQLNAEVATVLNISEDHMDRYSGLPAYHLAKHRIFRGAKQFVVNRQDALTRPLMGEGQPCWTFGLSKPD
FKAFGLREENGEKYLAFEFQNLMPVRELKIRGAHNQSNALAALALGHAVGLPFDAMLVALRTFAGLEHRCQWVRDLDGVG
YYNDSKATNVGAALAAIEGLGADIDGKVVLIAGGDGKGAEFKDLRDPVATHCRAVILMGRDSDKIGEAIGDAVPLIRATT
LVDAVAQCRAAAQPGDVVLLSPACASFDMFNNYEDRGHQFVRAVEDLA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
BJAB07104_00311 YP_008207776.1 UDP-N-acetylmuramoylalanine-D-glutamate ligase Not tested AbaR25 Protein 3e-90 55
BJAB0868_00315 YP_008211641.1 UDP-N-acetylmuramoylalanine-D-glutamate ligase Not tested AbaR26 Protein 3e-90 55