Gene Information

Name : Pfl01_4448 (Pfl01_4448)
Accession : YP_350176.1
Strain : Pseudomonas fluorescens Pf0-1
Genome accession: NC_007492
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 5008455 - 5009906 bp
Length : 1452 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence : 
ATGTCCGTCCGTCGTACCAAAATCGTCGCTACCCTTGGCCCGGCCAGTAACTCGCCGGAAGTTCTCGAACAGCTGATTCT
GGCTGGCCTGGACGTCGCCCGTCTGAACTTCTCCCACGGCACCCCCGACGAGCACAAGGCTCGCGCGAAGCTGGTGCGTG
ACCTCGCGGCCAAGCACGGTCGCTTCGTCGCCCTGCTGGGTGACCTGCAAGGCCCGAAAATCCGTATCGCCAAATTCGCC
AACAAGCGCATCGAGCTGAAGATCGGTGACCAGTTCACCTTCTCCACCAGCCATCCGCTGACCGAAGGCAACCAGCAAGT
GGTCGGCATCGACTACCCGGACCTGGTCAAGGACTGCGGCGTGGGCGACGAGCTCCTGCTCGATGACGGCCGCGTGGTCA
TGCGCGTTGAAACCGCCACCGCCACCGAGCTGAACTGCGTCGTGACCATCGGCGGTCCGCTGTCCGACCACAAAGGCATC
AACCGTCGCGGTGGCGGCCTGACCGCGCCGGCCCTGACTGAAAAAGACAAGGCCGACATCAAGCTCGCCGCTGAAATGGA
AGTCGACTACCTCGCCGTGTCCTTCCCGCGTGACGCCGCCGACATGAACTACGCCCGTCAACTGCGCGACGAAGCCGGCG
GCACCGCCTGGCTGGTGGCGAAGATCGAACGCGCCGAAGCCGTGGCCGACGACGAAACCCTCGACGGTCTGATCAAGGCT
TCCGACGCGGTGATGGTTGCCCGTGGTGACCTCGGTGTGGAAATCGGCGACGCCGAGCTGGTGGGCATCCAGAAGAAAAT
CATTCTGCACGCACGCCGCCACAACAAGGCGGTGATCGTGGCGACCCAGATGATGGAGTCGATGATCCAGAACCCGATGC
CGACCCGCGCCGAAGTGTCCGACGTGGCCAACGCCGTGCTCGACTACACCGACGCCGTGATGCTGTCTGCCGAGTCCGCT
GCCGGCGCTTACCCGCTGGAAGCCGTGCAGGCGATGGCGCGCATCTGTGTCGGCGCTGAAAAGCACCCGACCAGTAAGAC
CTCCAGCCACCGTATCGGCAAGGAATTCGAGCGCTGCGACGAAAGCATCGCGCTGGCGACCATGTACACCGCCAACCACT
TCCCGGGCGTCAAGGCGATCATCGCACTGACCGAAAGCGGCTACACCCCGCTGATCATGTCGCGTATCCGCTCCTCGGTG
CCGATCTACTGCTTCACCCCGCACCGAGAAGCCCAGGCTCGCGCGGCAATGTTCCGTGGCGTGTACACCGTACCGTTCGA
CCCGGCTTCGCTGGCACCGAACGAAGTCAGCCAGAAAGCCATCGACGAACTGGTCAAGCGCGGCGTAGTGGAGAAAGGCG
ACTGGGTCATCCTGACCAAGGGCGACAGCTACCACACCACCGGCGGCACCAACGGCATGAAGATCCTGCACGTGGGCGAT
CCGCAGGTCTGA

Protein sequence : 
MSVRRTKIVATLGPASNSPEVLEQLILAGLDVARLNFSHGTPDEHKARAKLVRDLAAKHGRFVALLGDLQGPKIRIAKFA
NKRIELKIGDQFTFSTSHPLTEGNQQVVGIDYPDLVKDCGVGDELLLDDGRVVMRVETATATELNCVVTIGGPLSDHKGI
NRRGGGLTAPALTEKDKADIKLAAEMEVDYLAVSFPRDAADMNYARQLRDEAGGTAWLVAKIERAEAVADDETLDGLIKA
SDAVMVARGDLGVEIGDAELVGIQKKIILHARRHNKAVIVATQMMESMIQNPMPTRAEVSDVANAVLDYTDAVMLSAESA
AGAYPLEAVQAMARICVGAEKHPTSKTSSHRIGKEFERCDESIALATMYTANHFPGVKAIIALTESGYTPLIMSRIRSSV
PIYCFTPHREAQARAAMFRGVYTVPFDPASLAPNEVSQKAIDELVKRGVVEKGDWVILTKGDSYHTTGGTNGMKILHVGD
PQV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 1e-77 45
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-59 44
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-60 44
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 4e-60 44
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 4e-60 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Pfl01_4448 YP_350176.1 pyruvate kinase VFG0479 Protein 2e-60 44