Gene Information

Name : Pfl01_3001 (Pfl01_3001)
Accession : YP_348730.1
Strain : Pseudomonas fluorescens Pf0-1
Genome accession: NC_007492
Putative virulence/resistance : Unknown
Product : DNA topoisomerase III
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0550
EC number : -
Position : 3442472 - 3444439 bp
Length : 1968 bp
Strand : +
Note : decatenates replicating daughter chromosomes

DNA sequence :
ATGCGTCTCTTCCTCTGCGAGAAACCATCCCAAGGTCGTGACATCGCCAAGGTTCTCGGGGCCACTCGGCGTGGTGACGG
TTGCCTGATCGGCACCGACTCAACCGTCACCTGGTGTATCGGTCACCTGCTGGAGACGGCCCCACCTGAAGCTTATGGTG
CTCAGTACAAATCCTGGTCGCTGGATCATCTACCGATCATTCCCGCGCAGTGGCAGGTCGAGGTCAAATCCAAGACCGCC
GCGCAGTTCAAAATCATCAAACGCCTGCTGAGTGAAGCCTCAAGTGTTGTCATCGCGACCGACGCCGATCGCGAAGGTGA
AATGATTGCCCGCGAGCTGCTTGAACTCTGCAGGTATCGAGGCCCCGTTCTGCGCCTCTGGTTGTCCGCCCTCAACGAGG
CGTCTATTCGCAAAGCCTTGTCCTCGCTGAAGTCCGGTCAGGTGACTTTCCCGCTCTATCACTCGGCGCTCGCCCGCAGC
CGTGCCGACTGGCTGATCGGCATGAACCTCAGTCGTTTGTTTACCCTTCTCGGTCGGCGAGCAGGTTACGACGGCGTTTT
GTCAGTGGGGCGCGTCCAGACACCCACTTTACGTCTGGTGGTCGATCGGGATCGTGCGATTGCCAGCTTCGTCCCGGTGC
CTTACTGGACCGTTGACGTGCACCTATCGTCGATGGGCCAGCGATTCATCGCGTCGTGGTTACCACCACGTTCAGGACGG
GACGAAGCGGGGCGTTGCCTGCAACAGGCGCTCGCCCATCACGCGGTCCAAACAATCACCGGCGAGAAGTCCGCCACGGT
ACTCTCGTTGCAAACTGAGCAGCTCCGAGAACCGCCGCCCCTGCCCTTCGACCTGAGCACGTTGCAAGAAGTCTGCTCGC
GTAAGCTGGGGCTCGGTGTTCAGGAAACCCTGAACATCGCCCAAGCCCTGTATGAAACCCACAAAGCCACCACCTACCCG
CGCAGCGATTGCCGCTATTTGCCAGAGAGCATGTTCAACGAGGCACCCGCGGTACTCGACGCCCTGCTCAAAACGGATCC
TGCACTCAGACCTGCATTCGGGAGCCTCGATCGATCATTGCGCGCCCGCGTATGGAACAATGCTAAGGTCACCGCGCACC
ACGGCATCATTCCCACCACCGAGCCCGCAAATTTGGTGCGGATGTCCGAACAAGAACGCCAAGTCTACGAACTGATCCGC
AGCCACTACCTGGCGCAGTTTCTTCCGCATCATGAGTTTGATCGAACCCAGGTCGAACTGGAATGTAGCAAAGAACGTTT
GACCGCTGTCGGCAAACAGATCCTGATCCAGGGCTGGAAAGGCTTGCTCACCGAAAACACCGAGGACGATGAGCCCAGTC
AAAAGCACCAAGTATTGCCAGTCCTGCAACAGGGTACTCAGTGCGCCGTAAACGACGTCGAACTCAAGTCAATGCGCACC
GTCGCTCCCAAACCGCTCACCGAGGGCGATCTGATCAAGGCGATGAAAAACGTCGCCAAGCTGGTCAACGACCCACGCCT
GAAACAGAAACTTCGGGACACCACCGGTATCGGTACCGAAGCCACGCGAGCCGGCATTATCAAAGGGTTGGTTGATCGCG
GTTATTTGCTGAAGAAAAAACGCGCCTTAATGGCCTCCGCCGCGGCCCATACCCTGATCGAGGCGGCACCGACAGCAGTC
GCCGATCCGGGTATGACCGCAATCTGGGAACAGGCGCTGGACGAAATCGAAGCGGGACGCCTGACCCTGGATGCGTTCGT
TGCCAAGCAGGCGAACTGGATTGCGCAGTTGGTCGCACACTACCGCTCGCACACTTTGGCAATAGCGGTCGAAACGAGCC
CGAGCTGCCCTATGTGCAACGCCTCAATGCTCAGGCGAAAGGGAAAATCAGGGCCGTTCTGGTCGTGCTCCTGCTACCCG
GATTGCAAGGGCACTGTGTCGATCAGTAAAGATACGCGTCGCTCATGA

Protein sequence :
MRLFLCEKPSQGRDIAKVLGATRRGDGCLIGTDSTVTWCIGHLLETAPPEAYGAQYKSWSLDHLPIIPAQWQVEVKSKTA
AQFKIIKRLLSEASSVVIATDADREGEMIARELLELCRYRGPVLRLWLSALNEASIRKALSSLKSGQVTFPLYHSALARS
RADWLIGMNLSRLFTLLGRRAGYDGVLSVGRVQTPTLRLVVDRDRAIASFVPVPYWTVDVHLSSMGQRFIASWLPPRSGR
DEAGRCLQQALAHHAVQTITGEKSATVLSLQTEQLREPPPLPFDLSTLQEVCSRKLGLGVQETLNIAQALYETHKATTYP
RSDCRYLPESMFNEAPAVLDALLKTDPALRPAFGSLDRSLRARVWNNAKVTAHHGIIPTTEPANLVRMSEQERQVYELIR
SHYLAQFLPHHEFDRTQVELECSKERLTAVGKQILIQGWKGLLTENTEDDEPSQKHQVLPVLQQGTQCAVNDVELKSMRT
VAPKPLTEGDLIKAMKNVAKLVNDPRLKQKLRDTTGIGTEATRAGIIKGLVDRGYLLKKKRALMASAAAHTLIEAAPTAV
ADPGMTAIWEQALDEIEAGRLTLDAFVAKQANWIAQLVAHYRSHTLAIAVETSPSCPMCNASMLRRKGKSGPFWSCSCYP
DCKGTVSISKDTRRS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
topB AAN62194.1 putative DNA topoisomerase III Not tested PAGI-2(C) Protein 0.0 66
topB AAN62317.1 putative DNA topoisomerase III Not tested PAGI-3(SG) Protein 0.0 65
pph56 CAI36097.1 Topoisomerase IA Not tested PPHGI-1 Protein 0.0 64
topB NP_458624.1 topoisomerase B Not tested SPI-7 Protein 1e-174 52
topB NP_807836.1 DNA topoisomerase III Not tested SPI-7 Protein 1e-174 52
topB AAF14809.1 topoisomerase B Not tested SPI-7 Protein 1e-174 52
PMI2630 YP_002152332.1 DNA topoisomerase III Not tested Not named Protein 1e-160 49
topB1 YP_005176228.1 DNA topoisomerase III Not tested ICEPmu1 Protein 3e-164 49
topB AAW83109.1 TopB Not tested GGI Protein 1e-126 41