Gene Information

Name : lysS (Ava_3150)
Accession : YP_323653.1
Strain : Anabaena variabilis ATCC 29413
Genome accession: NC_007413
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3912207 - 3913892 bp
Length : 1686 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCGGAAGAAGATATCCGTGCCGCTAGGCTGGAGAAAGTAGAACAACTCAAGCAGCTAGGAACTAACCCCTACGCTTA
CCGTTGGGAGTCTACCCATCACGCCGCCCAATTGCAAGAAAAATTTGCTGATTTAACCAGTGGTGAAGAAGTCGATACAG
AAGTTGCCATTGCCGGACGCATTATGGCGCGTCGCGTTTTCGGTAAATTGGCTTTCTTCACCTTGGAAGATGAAACAGGC
ACTATTCAGCTTTATTTAGAGAAAAATCGTATCCAAGAAAGCATGGCAGAAACTGATGCCGATGCTTTTAATCATCTCAA
ACAACTCACAGATGTAGGAGATATTCTGGGAGCCAAGGGAACCATAAAACGGACTGAAAAGGGCGAGTTATCGATTTACG
TCAAAGAATATACCATCCTCACCAAATCCCTACTGCCGCTACCCGACAAGTGGCATGGGTTAACGGATGTTGCCAAGCGC
TACCGTCAACGCTACGTTGATTTAATTGTTAACCCCGAAGTCCGCCAAACTTTCCGCCGTCGCGCCCAAATTACGGCTGG
GATTCGCCGCTATTTGGAACAAAGGGATTTTCTAGAGATTGAAACCCCAGTTTTGCAAAGTGAAACTGGTGGTGCTGATG
CGCGTCCCTTCATCACTTACCACAACACCTTAGAAATGGAGTTGTATCTGCGGATTGCCACAGAACTCCACCTCAAACGG
TTGATTGTCGGTGGTTTTGAAAAAGTATTTGAGTTGGGGCGCGTTTTCCGCAATGAGGGAATTTCTACCAGACACAACCC
TGAATTTACCTCAATTGAAATTTACCAAGCCTACGCCGACTACAACGATATGATGGCGCTAACAGAAGGTATTATCACCA
CTGTTGCCCAAGATGTACTCGGTACGCTGCAAATTACCTACCAAGGCGAAACGGTAGATTTGACACCACCTTGGCGACGG
GTGACAATGCACGATTTAGTGAAAGAATATACGGGCTTGGATTTCCATTCTTTCCAAACACTAGAAGAAGCGAAATCAGC
AAGTAAAAATGCTGGAATTCCTGGTGTAGATGAAGCCCAAACTATAGGAAAAATCCTGAATTTAGCATTTGAAGAAAAGG
TAGAAGCTAACTTAATTCAGCCGACCTTTGTCATTGATTATCCTGTAGAAATTTCGCCATTAGCTAAACCTCACCGTTCT
CAACCTGGGTTAGTAGAAAGATTTGAATTATTCATAGTCGGGCGGGAGACTGCGAATAGTTTCTCAGAATTAACAGACCC
CATCGACCAAAGAGAACGTTTGGAAGCCCAAGCAGCCAAAAAAGCCGCCGGTGACTTAGAAGCCCAAGGTGTAGATGAAG
ATTTCTTAACTGCGTTGGAATATGGTATGCCACCCACAGGCGGTTTAGGTATTGGGATTGATCGGTTAGTCATGTTATTA
ACTGATTCTGCCAGCATTCGAGATGTAATTGCTTTCCCTCTACTCAAACCCGAAGGCAGCTTTATTAAGGAATATCGCTA
TGAGTCAACAACTCAAACACTGACTATGGAGTTTGATAGTGGAAGTGTTTACGAATATTTCAAAGTACCGCTTACTGTCA
AGGAAGAATTAGATAATGCACCGTCTAAGGGTCAATATTTCCATAAGTTTATTAAAGGGAAATTCAAGTATGAACAATTG
AGTTGA

Protein sequence :
MSEEDIRAARLEKVEQLKQLGTNPYAYRWESTHHAAQLQEKFADLTSGEEVDTEVAIAGRIMARRVFGKLAFFTLEDETG
TIQLYLEKNRIQESMAETDADAFNHLKQLTDVGDILGAKGTIKRTEKGELSIYVKEYTILTKSLLPLPDKWHGLTDVAKR
YRQRYVDLIVNPEVRQTFRRRAQITAGIRRYLEQRDFLEIETPVLQSETGGADARPFITYHNTLEMELYLRIATELHLKR
LIVGGFEKVFELGRVFRNEGISTRHNPEFTSIEIYQAYADYNDMMALTEGIITTVAQDVLGTLQITYQGETVDLTPPWRR
VTMHDLVKEYTGLDFHSFQTLEEAKSASKNAGIPGVDEAQTIGKILNLAFEEKVEANLIQPTFVIDYPVEISPLAKPHRS
QPGLVERFELFIVGRETANSFSELTDPIDQRERLEAQAAKKAAGDLEAQGVDEDFLTALEYGMPPTGGLGIGIDRLVMLL
TDSASIRDVIAFPLLKPEGSFIKEYRYESTTQTLTMEFDSGSVYEYFKVPLTVKEELDNAPSKGQYFHKFIKGKFKYEQL
S

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 4e-108 48
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 3e-84 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_323653.1 lysyl-tRNA synthetase VFG1668 Protein 2e-108 48