Gene Information

Name : fliI (Nwi_0526)
Accession : YP_317145.1
Strain : Nitrobacter winogradskyi Nb-255
Genome accession: NC_007406
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 584205 - 585530 bp
Length : 1326 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGAAGGGCCTGGCCGGGCGGATTGGTGAGATCGAGCGCGTCAATATTTATGGCCGCGTGGTCGGCGTACGCGGACTGAT
GGTCGAGATAGCGGGGCCCATCCATGCGATGTCGGTGGGAGCGCGCATCGTGATCGAAACCGGGGTCGGTCGGACCATCC
CGGCGGAAGTGGTCGGTTTCAACGGCGACAATGCTGTCGTCATGCCGTTCGCCAGCCTGGACGGGGTGCGGCGCGGCTGC
CGCGCCCTGGTCGCCAATGCTTCCGACAGTGTACGGCCGGCAGCCGCATGGCTCGGGCGGGTTCTCAACGCCCTCGGAGA
GCCGATCGACGGCAAGGGGCCGCTCCCGCAGGGCCCGACGCCCGTACCGTACCGCAATTCGCCACCGTCGGCGCATTCGC
GCCAACGGGTCGGCCAGCCGCTCGATCTCAAGGTTCGCGCCCTGAACACGTTCCTGACCTGCTGCCGCGGTCAGCGGCTC
GGTATTTTCGCGGGCTCCGGCGTCGGCAAATCGGTGCTGCTGTCGATGTTGGCGCGCAACGCCGATGCCGACGTTTCGGT
GATCGGGCTGATCGGCGAACGCGGACGCGAAGTGCAGGAGTTCCTGCAGGACGATCTCGGCGACGAGGGGCTCGCCCGAT
CGGTCGTCGTGGTCGCAACCTCCGATGAGCCCGCCCTGATGCGGCGGCAGGCTGCCTATCTGACCCTGGCGATTGCGGAA
TTCTTTCGCGACGAGGGCAGGGACGTGATGTGCATGATGGACTCGGTGACGCGCTTTGCCATGGCGCAGCGCGAAATCGG
ACTGTCGACCGGCGAGCCGCCGACCACGAAGGGTTATACGCCAACCGTCTTCACCGAGTTGCCGAGGCTTTTGGAGCGCG
CCGGGCCCGGAGCCGACAAGGGGACAATAACCGGTATCTTCACGGTGCTGGTCGATGGTGACGATCACAACGAGCCGGTC
GCCGACGCGGTGCGCGGCATTCTCGACGGGCACATCGTGATGGAGCGCGCCATTGCCGAGCGGGGCCGCTATCCCGCGAT
CAATATTTTGAAGTCAGTGTCGCGCACGATGCCGAGATCGGCCGATCCGGCTTTCCTGCCGGACATTACCTGCGCGCGGC
GGATGATGGCCACTTATTCCGACATGGAGGAATTGATCCGGCTCGGCGCCTATCGGGCCGGCTCCAACGCGGAGGTCGAC
GAGGCCATCAGGTTGAATCCGGTCCTGGAGGATTTCCTGCGTCAGGCGAAGGACGAAGTCACCAGCATCGAGGACGGTTA
TCGAAGATTGCAGCAAATCGTCAACACTTTAGCAACGGAAAGCTAA

Protein sequence :
MKGLAGRIGEIERVNIYGRVVGVRGLMVEIAGPIHAMSVGARIVIETGVGRTIPAEVVGFNGDNAVVMPFASLDGVRRGC
RALVANASDSVRPAAAWLGRVLNALGEPIDGKGPLPQGPTPVPYRNSPPSAHSRQRVGQPLDLKVRALNTFLTCCRGQRL
GIFAGSGVGKSVLLSMLARNADADVSVIGLIGERGREVQEFLQDDLGDEGLARSVVVVATSDEPALMRRQAAYLTLAIAE
FFRDEGRDVMCMMDSVTRFAMAQREIGLSTGEPPTTKGYTPTVFTELPRLLERAGPGADKGTITGIFTVLVDGDDHNEPV
ADAVRGILDGHIVMERAIAERGRYPAINILKSVSRTMPRSADPAFLPDITCARRMMATYSDMEELIRLGAYRAGSNAEVD
EAIRLNPVLEDFLRQAKDEVTSIEDGYRRLQQIVNTLATES

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 5e-74 49
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-74 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-74 48
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-74 48
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-75 48
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-80 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-80 47
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-80 47
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-80 47
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 3e-81 47
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-81 47
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-81 47
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-81 47
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-80 47
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-74 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-84 46
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-74 44
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-75 44
escN AAK26715.1 EscN Virulence LEE Protein 6e-75 44
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 3e-75 44
escN AAL57542.1 EscN Virulence LEE Protein 6e-75 44
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-75 44
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-75 44
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-75 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-75 44
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-75 44
escN AAC38383.1 EscN Virulence LEE Protein 1e-75 44
escN AAC31513.1 L0034 Virulence LEE Protein 8e-76 44
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 8e-76 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-72 43
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-72 43
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-70 42
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-63 41
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-63 41
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 2e-62 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_317145.1 flagellum-specific ATP synthase VFG0515 Protein 1e-74 48
fliI YP_317145.1 flagellum-specific ATP synthase VFG1254 Protein 2e-83 47
fliI YP_317145.1 flagellum-specific ATP synthase VFG2499 Protein 1e-81 47
fliI YP_317145.1 flagellum-specific ATP synthase VFG2144 Protein 3e-85 46
fliI YP_317145.1 flagellum-specific ATP synthase VFG2331 Protein 2e-79 45
fliI YP_317145.1 flagellum-specific ATP synthase VFG0048 Protein 2e-83 45
fliI YP_317145.1 flagellum-specific ATP synthase VFG0192 Protein 1e-81 44
fliI YP_317145.1 flagellum-specific ATP synthase VFG0812 Protein 4e-76 44
fliI YP_317145.1 flagellum-specific ATP synthase VFG0730 Protein 7e-76 44
fliI YP_317145.1 flagellum-specific ATP synthase VFG0390 Protein 1e-82 42