Gene Information

Name : pykA (Tbd_0162)
Accession : YP_313920.1
Strain : Thiobacillus denitrificans ATCC 25259
Genome accession: NC_007404
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 174936 - 176369 bp
Length : 1434 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGATTCGCAGAACCAAAATCGTCGCCACCCTCGGCCCCGCCTGCTCCGACGCCAAGACGCTCGATCGGATGATGGAAGC
CGGCCTGGACGTGGTCCGTCTCAACTTCTCCCACGGCGTGGCGCAGGACCACATCGACCGCGCCGAACTCGTGCGTTCGC
TCGCGCGTACGCGCGGTCGCGCGGTCGGCGTCCTCGTCGACCTGCAGGGGCCGAAGATCCGCATCGGCAAATTCAAGGAC
AACAAGATCGTCCTCGCCAAGGGCGACAACTTCATCCTCGACGCGGAATGCGCGCTCGGCGACCAGGAGCGCGTCGGCCT
CGACTACAAGGAATTGCCGAGCGACGTCGCCCGTGGCGTGACGCTCCTGCTCGACGACGGCCGGATCGAACTCTGGGTCG
AAGACGTCAAGGGCAGCGAAATCCACTGCAAGGTCGTGCAGGGCGGCGTGCTCTCGAATAACAAGGGCATCAACCGCAAG
GGCGGCGGTCTTTCGGCCGCCGCGCTGACCGAGAAGGACATCGAGGACATCAAGACCGCCGCGGCGCTCAAGGCCGACTA
CCTCGCGGTCTCCTTCCCGCGCTCGGGCGCCGACATGCGCCAGGCGCGCGAGTTGCTGCGGGCCGCGGGCGGCAGGAGCC
AGCTCGTCGCCAAGATCGAGCGCACTGAAGCGATCGAGAACCTCGAAGAAATCCTCGATGCGTCCGACGCGATCATGGTC
GCCCGCGGCGACCTCGGCGTCGAGGTCGGCGACGCTGCGGTGCCGGCGCTGCAGAAGCGCATGATCCGTATGGCGATCGC
GCGCAACAAGGTCGTCATCACCGCGACGCAGATGATGGAGTCGATGATCACGAGCCCGATCCCGACCCGCGCCGAAGTCT
CCGACGTCGCCAACGCCGTGCTCGACGGCACCGACGCCGTCATGCTCTCGGCCGAGACCGCGGCCGGGCAATACCCGGTC
GAAGCCGTGCAGGCGATGGACCGCGTCTGCCTCGAAGCCGAAAAGGAAGCCGAGACGCAGTTCACGCGCGAGCGGCTCGA
CCACAGCTTCCTGCGCGCCGACGAGGCGATCGCGATGTCGGCCGTGTTCACCTCGGTGCACCTCAACATCAAGGCCATCG
CCGCGCTGACCGAGTCCGGCAATACCTGTCTGTGGATGAGCCGCCTGTCGACGCGCGTGCCGATCTACGCCTTGACACCT
CAGGTCGAAACCCGTAGAAAAGTCACTCTCTTCCGCGGTGTCTATCCGATCAACCTCAAGACCGCCACCAAGGAGCGCGA
CGTGCTGCTCGCCGAGGCCGAGGAAGAATTGCGGCGGCGCGGCGCCGTGCGCGACGGCGACCTGATCCTGCTGACGATCG
GCGAGCCGATCGGCCAGTCGGGCGGCACCAATACGATGAAGATCGTCCGTGTGGGCGCTTCGAAGAAACCCTGA

Protein sequence :
MIRRTKIVATLGPACSDAKTLDRMMEAGLDVVRLNFSHGVAQDHIDRAELVRSLARTRGRAVGVLVDLQGPKIRIGKFKD
NKIVLAKGDNFILDAECALGDQERVGLDYKELPSDVARGVTLLLDDGRIELWVEDVKGSEIHCKVVQGGVLSNNKGINRK
GGGLSAAALTEKDIEDIKTAAALKADYLAVSFPRSGADMRQARELLRAAGGRSQLVAKIERTEAIENLEEILDASDAIMV
ARGDLGVEVGDAAVPALQKRMIRMAIARNKVVITATQMMESMITSPIPTRAEVSDVANAVLDGTDAVMLSAETAAGQYPV
EAVQAMDRVCLEAEKEAETQFTRERLDHSFLRADEAIAMSAVFTSVHLNIKAIAALTESGNTCLWMSRLSTRVPIYALTP
QVETRRKVTLFRGVYPINLKTATKERDVLLAEAEEELRRRGAVRDGDLILLTIGEPIGQSGGTNTMKIVRVGASKKP

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 3e-91 52
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 6e-65 41
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 6e-65 41
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-64 41
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 7e-65 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA YP_313920.1 pyruvate kinase VFG0479 Protein 3e-65 41