Gene Information

Name : lysS (MHJ_0203)
Accession : YP_279005.1
Strain : Mycoplasma hyopneumoniae J
Genome accession: NC_007295
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 228897 - 230366 bp
Length : 1470 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATTATGTCAAAACTAAATGATCAACAACAATTTCGCCGGGATAAGCTTAAAAATTTAGTTAAAAATGGTTTTAATTTCCC
TAGTTCAACTTTTGAGCATGATAATTTAGTAGAAATAAATGAAAAATTTAGTCAAAAATCGAAGGAATTTTTCCTTGAAA
ATCAAGTAAAAATTGCTTTTGCTGGTCGACTTATTCGCCAACGCGGCCCATTTTTTATAATTTTTAGTCAAAACTTGCAA
TTTCAAGCTTATATTTCAAAAGAATTTCAAAAAAAAAATGAATTTATCTTTGCAAATTTAGATCTTGGTGATATAATTGA
GGTTTCAGGTTATCTTTTCAAGACGCAAACTGGTCAACTTAGTATTAAAGTTAATAATTTTTCTCTGCTTACAAAGTCCT
TGCACCCTTTACCAGATCAGTATTATGGTATTGAAAATCCAGATGAGAAATACAGGAAACGTTATCTTGACCTGCTTGTA
AATTCAGAATCTGCAAAAACTTTTCGACTTAGAAGTAAAATTATCTCCTTAATCCGTACATTTTTTGATTCCCAAGGTTT
TTTAGAAGTTGATACTCCCGTTTTACAGCCAGTTTTAGGCGGGGCATCAGCAAAACCTTTTATTACTTATTATAATTCTC
TTAGTCAAAATTTTTATCTCCGAATTGCCACGGAATTACCGCTGAAAAAATTATTAGTTGCCGGTTTTGACCGTGTCTAT
GAAATTGGTAAAATTTTTCGTAATGAAGGTTTTGATTCAACTCATAATCCCGAATTCACCTCAATTGAATTTTATCAGGC
TTATGCAAATTTAGAGAAAATAATGGATCAAACCGAAAATTTATTTCGTTTTTTATTTGAAAAATTAAATCTTGATCCGG
CAAATTTTGATTTTTCAAATAAAAAAATTAATTTCTTGGAAAAATTTGCCCGTTATGATATGATTGAGATTACTTCAAAA
TTAATGAACTTCGATTTAAAAAGTGCTAATTTTGCTGATTTAGTCGAAAAAGCAAAAAAAGAAGGGGTGAAGATCGAGCC
ATTTTTCAAAAAAGGGCATCTAATCAACAAATTTTTTGAAAAATTTGTTGAACCAACACTAATAAATCCAACTTTTATCA
TAGGTCATCCAATTGAAATTTCCCCGCTAGCCAAGTCAAATCCAAACAATCCTAATTTTACTCTCAGGGCCGAATTATTT
ATCTGTGGTAAAGAATTTGCGAATATGTTTGATGAATTAAATGATCCAATTGACCAATTAAGTCGTTTTCAAGCCCAAAT
TATCGAAAAAAATCAGGGAAATCAAGAAGCAAGTGAGATCGACAATGAATTTGTTCAAGCACTTGAATATGGAATGCCAC
CCGCCGGGGGTTGTGGCATTGGCATTGACAGATTGACAATGCTTTTAACAAAGAATGAATCAATCCGTGAGGTAATTTTA
TTCCCACAGCTAAAACCAAAAAAGGACTAA

Protein sequence :
MMSKLNDQQQFRRDKLKNLVKNGFNFPSSTFEHDNLVEINEKFSQKSKEFFLENQVKIAFAGRLIRQRGPFFIIFSQNLQ
FQAYISKEFQKKNEFIFANLDLGDIIEVSGYLFKTQTGQLSIKVNNFSLLTKSLHPLPDQYYGIENPDEKYRKRYLDLLV
NSESAKTFRLRSKIISLIRTFFDSQGFLEVDTPVLQPVLGGASAKPFITYYNSLSQNFYLRIATELPLKKLLVAGFDRVY
EIGKIFRNEGFDSTHNPEFTSIEFYQAYANLEKIMDQTENLFRFLFEKLNLDPANFDFSNKKINFLEKFARYDMIEITSK
LMNFDLKSANFADLVEKAKKEGVKIEPFFKKGHLINKFFEKFVEPTLINPTFIIGHPIEISPLAKSNPNNPNFTLRAELF
ICGKEFANMFDELNDPIDQLSRFQAQIIEKNQGNQEASEIDNEFVQALEYGMPPAGGCGIGIDRLTMLLTKNESIREVIL
FPQLKPKKD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-85 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_279005.1 lysyl-tRNA synthetase VFG1668 Protein 6e-86 41