Gene Information

Name : dnaB (Psyc_0959)
Accession : YP_264246.1
Strain : Psychrobacter arcticus 273-4
Genome accession: NC_007204
Putative virulence/resistance : Unknown
Product : primary replicative DNA helicase
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0305
EC number : 3.6.1.-
Position : 1144536 - 1145939 bp
Length : 1404 bp
Strand : -
Note : The N-terminal domain is required both for interaction with other proteins in the primosome and for DnaB helicase activity. The C-terminal domain contains an ATP-binding site and is therefore probably the site of ATP hydrolysis.

DNA sequence :
ATGGCAGATACTGAAAAAACCGACGACTTACTCAATCAAACACCGCCGCACAATATCGATATTGAAAAGGCATTGCTAGC
GTCTTTGATGAGTATCGAAGAGGCATATGACAAAATTGCAGATATTGTCACCAAAGACGATTTTTATGCTGGACGACACC
AGTATATTTTTGATGCCATTGCTCATTTAGCCGCCGTCAATGAGCCTTATGATACCGTCATGGTGCACGATTGGTTAGAG
GCACAAAAACTGCTCAAAAGCGCAGGTGGTGACAGTTATTTGGCAGATATTTTGAGCCAAAGCCCTGCCACCTTATTTAA
CCTGACCGCTTACGCCCAGCGCGTGCGTGAGCTGTCTACGCTACGTCAGCTCATTACCACCGGTAATGATATGTTGACCC
TAGCCTACAACCCCAAAGACCAAACCGTCAGCGATATTTTGGACAACGTAGAAGCAAAAATATTTAGTATCAATGAGCAA
CATAACAAACGTGCTGAGCAGCGTGGACCTGTAGGTATTACCGCCGTATTGACCAATGTCCTTGATAAAATCCAAGAACT
TAAATCAAACCCAGATGGCATGATTGGCTTACAAACACCATTTGTTGAGCTCAATAATAAAACCCAAGGCTTGCAAGCAG
GCGATTTAGTCATTGTGGCTGCCCGTCCATCGATGGGTAAAACCACCTTTGCCATGAACTTAGCAGAAGGGATTTTATTT
AATGAAGATTTGCCCGTGGTGGTATTTTCGATGGAGATGCCCGCAGAATCTATCGTGATGCGCTTGCTGTCATCGTGGGG
CGCCATCAATCAGACGCACCTGCGTTCTGGGCAAATGAACGAAGATGAATGGGCAAAAATGATGAACGCCATTCAGCATT
TGCAGTCAAAGCATCTATATATTGATGACAGTACTGCCCTGCCACCATCTGAGCTGCGCTCACGCTGTCGCCGTATTGCC
AAAAACCATGATGGCAAGCTTGGCGCTATCATTGTCGATTACCTACAATTAATGAAAGTACCAGGTCTAGATGGTAACCG
TGTTGGAGAGATCTCTGAGATTTCTCGGAGCTTAAAAGCGCTGGCACGTGAGCTTGAATGTCCGGTTATCGCCTTATCGC
AGCTTAACCGTAGCCTAGAAAATCGCCCTAACAAACGCCCTATTATGTCAGATCTGCGTGAATCTGGTGCGATTGAGCAG
GATGCCGATTTGATTATGTTCATCTATCGTGATGAGGTTTATAATGAGAATACAGACCATAAAGGGGTGGCTGAAATTAT
CATTGGTAAACAGCGTAATGGTCCTATCGGCACCATACGTTTAGGTTTTGAAGGGCAATTTACCCGCTTTATCAATTTGA
CTCCAGAATACTATCAAAACGTCAGTTTCGAGAATGACGAATAA

Protein sequence :
MADTEKTDDLLNQTPPHNIDIEKALLASLMSIEEAYDKIADIVTKDDFYAGRHQYIFDAIAHLAAVNEPYDTVMVHDWLE
AQKLLKSAGGDSYLADILSQSPATLFNLTAYAQRVRELSTLRQLITTGNDMLTLAYNPKDQTVSDILDNVEAKIFSINEQ
HNKRAEQRGPVGITAVLTNVLDKIQELKSNPDGMIGLQTPFVELNNKTQGLQAGDLVIVAARPSMGKTTFAMNLAEGILF
NEDLPVVVFSMEMPAESIVMRLLSSWGAINQTHLRSGQMNEDEWAKMMNAIQHLQSKHLYIDDSTALPPSELRSRCRRIA
KNHDGKLGAIIVDYLQLMKVPGLDGNRVGEISEISRSLKALARELECPVIALSQLNRSLENRPNKRPIMSDLRESGAIEQ
DADLIMFIYRDEVYNENTDHKGVAEIIIGKQRNGPIGTIRLGFEGQFTRFINLTPEYYQNVSFENDE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed ABR13456.1 replicative DNA helicase Not tested PAGI-5 Protein 5e-90 47
dnaB CAI36044.1 replicative DNA helicase DnaB Not tested PPHGI-1 Protein 1e-78 46
dnaB2 YP_001007676.1 putative DNA helicase Not tested YAPI Protein 2e-81 44
PMI2640 YP_002152342.1 DNA helicase Not tested Not named Protein 3e-77 43
api2 CAF28476.1 putative DNA binding protein with DNA-dependent ATPase activity Not tested YAPI Protein 1e-80 43
STY4522 NP_458617.1 DNA helicase Not tested SPI-7 Protein 1e-77 42
t4227 NP_807829.1 DNA helicase Not tested SPI-7 Protein 1e-77 42
dnaB2 YP_001402701.1 replicative DNA helicase Not tested YAPI Protein 8e-81 42